ID   HOKE_ECOLI              Reviewed;          50 AA.
AC   P77091; Q9R7T7;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Toxic protein HokE;
GN   Name=hokE {ECO:0000303|PubMed:10361310}; Synonyms=ybdY;
GN   OrderedLocusNames=b4415, JW5084;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   DISCUSSION OF SEQUENCE.
RX   PubMed=10361310; DOI=10.1046/j.1365-2958.1999.01431.x;
RA   Pedersen K., Gerdes K.;
RT   "Multiple hok genes on the chromosome of Escherichia coli.";
RL   Mol. Microbiol. 32:1090-1102(1999).
RN   [6]
RP   REGULATION BY LEXA, AND INDUCTION.
RC   STRAIN=K12 / RW118;
RX   PubMed=10760155; DOI=10.1046/j.1365-2958.2000.01826.x;
RA   Fernandez De Henestrosa A.R., Ogi T., Aoyagi S., Chafin D., Hayes J.J.,
RA   Ohmori H., Woodgate R.;
RT   "Identification of additional genes belonging to the LexA regulon in
RT   Escherichia coli.";
RL   Mol. Microbiol. 35:1560-1572(2000).
CC   -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system; if
CC       it expressed it could be neutralized by antisense antitoxin RNA SokE.
CC       {ECO:0000305|PubMed:10361310}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0ACG4}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P0ACG4}.
CC   -!- INDUCTION: Repressed by LexA, induced by DNA damage.
CC       {ECO:0000269|PubMed:10760155}.
CC   -!- MISCELLANEOUS: Interrupted by an IS186 element, which silences the
CC       locus in strain K12 MG1655. {ECO:0000305|PubMed:10361310}.
CC   -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB40780.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U82598; AAB40780.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAT48125.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35222.2; -; Genomic_DNA.
DR   RefSeq; WP_000956455.1; NZ_STEB01000055.1.
DR   RefSeq; YP_025295.1; NC_000913.3.
DR   AlphaFoldDB; P77091; -.
DR   SMR; P77091; -.
DR   BioGRID; 4260712; 218.
DR   STRING; 511145.b4415; -.
DR   PaxDb; P77091; -.
DR   EnsemblBacteria; AAT48125; AAT48125; b4415.
DR   EnsemblBacteria; BAA35222; BAA35222; BAA35222.
DR   GeneID; 2847715; -.
DR   KEGG; ecj:JW5084; -.
DR   KEGG; eco:b4415; -.
DR   PATRIC; fig|511145.12.peg.606; -.
DR   EchoBASE; EB4049; -.
DR   eggNOG; ENOG50336J3; Bacteria.
DR   eggNOG; ENOG5033FBI; Bacteria.
DR   HOGENOM; CLU_177638_3_2_6; -.
DR   BioCyc; EcoCyc:MON0-1581; -.
DR   PRO; PR:P77091; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR   InterPro; IPR000021; Hok/gef_toxin.
DR   InterPro; IPR018084; Hok/gef_toxin_CS.
DR   Pfam; PF01848; HOK_GEF; 1.
DR   PRINTS; PR00281; HOKGEFTOXIC.
DR   PROSITE; PS00556; HOK_GEF; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   CHAIN           1..50
FT                   /note="Toxic protein HokE"
FT                   /id="PRO_0000199036"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   50 AA;  5564 MW;  8AD9A981A6D46835 CRC64;
     MLTKYALAAV IVLCLTVLGF TLLVGDSLCE FTVKERNIEF KAVLAYEPKK