HOK_ECOLX
ID HOK_ECOLX Reviewed; 52 AA.
AC P11895;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Protein Hok;
GN Name=hok;
OS Escherichia coli.
OG Plasmid IncFII R1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CSH50; PLASMID=IncFII R1;
RX PubMed=3312951; DOI=10.1007/bf00329846;
RA Rasmussen P.B., Gerdes K., Molin S.;
RT "Genetic analysis of the parB+ locus of plasmid R1.";
RL Mol. Gen. Genet. 209:122-128(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CSH50; PLASMID=IncFII R1;
RX PubMed=1707122; DOI=10.1111/j.1365-2958.1990.tb02029.x;
RA Gerdes K., Thisted T., Martinussen J.;
RT "Mechanism of post-segregational killing by the hok/sok system of plasmid
RT R1: sok antisense RNA regulates formation of a hok mRNA species correlated
RT with killing of plasmid-free cells.";
RL Mol. Microbiol. 4:1807-1818(1990).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=CSH50; PLASMID=IncFII R1;
RX PubMed=3019679; DOI=10.1002/j.1460-2075.1986.tb04459.x;
RA Gerdes K., Bech F.W., Joergensen S.T., Loebner-Olesen A., Rasmussen P.B.,
RA Atlung T., Boe L., Karlstrom O., Molin S., von Meyenburg K.;
RT "Mechanism of postsegregational killing by the hok gene product of the parB
RT system of plasmid R1 and its homology with the relF gene product of the E.
RT coli relB operon.";
RL EMBO J. 5:2023-2029(1986).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system. Part
CC of the plasmid-stabilizing activity of plasmid R1; when R1 is lost
CC cells die rapidly. When overexpressed kills cells within 5 minutes;
CC causes collapse of the transmembrane potential and arrest of
CC respiration (PubMed:3019679). Its toxic effect is partially neutralized
CC by antisense RNA Sok. {ECO:0000269|PubMed:3019679, ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:3019679};
CC Single-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR EMBL; X05813; CAA29259.1; -; Genomic_DNA.
DR EMBL; A00659; CAA00075.1; -; Unassigned_DNA.
DR PIR; S00301; S00301.
DR RefSeq; NP_863052.1; NC_004998.1.
DR RefSeq; NP_957589.1; NC_005327.1.
DR RefSeq; WP_001312861.1; NZ_WWEV01000072.1.
DR RefSeq; YP_001096459.1; NC_009133.1.
DR RefSeq; YP_003108239.1; NC_013121.1.
DR RefSeq; YP_003108294.1; NC_013122.1.
DR RefSeq; YP_003937776.1; NC_014615.1.
DR RefSeq; YP_006954261.1; NC_019095.1.
DR RefSeq; YP_006990749.1; NC_019424.1.
DR RefSeq; YP_008997969.1; NC_023315.1.
DR RefSeq; YP_009066471.1; NC_025106.1.
DR RefSeq; YP_009068364.1; NC_025139.1.
DR RefSeq; YP_009071075.1; NC_025177.1.
DR RefSeq; YP_190154.1; NC_006671.1.
DR AlphaFoldDB; P11895; -.
DR SMR; P11895; -.
DR GeneID; 58462868; -.
DR OMA; NIRIASY; -.
DR OrthoDB; 2010283at2; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000021; Hok/gef_toxin.
DR InterPro; IPR018084; Hok/gef_toxin_CS.
DR Pfam; PF01848; HOK_GEF; 1.
DR PRINTS; PR00281; HOKGEFTOXIC.
DR PROSITE; PS00556; HOK_GEF; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Plasmid;
KW Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT CHAIN 1..52
FT /note="Protein Hok"
FT /id="PRO_0000199042"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 52 AA; 6082 MW; DFF2E2175DB72086 CRC64;
MKLPRSSLVW CVLIVCLTLL IFTYLTRKSL CEIRYRDGHR EVAAFMAYES GK
