ID   HOL24_BPSPP             Reviewed;          83 AA.
AC   O48470;
DT   12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   23-FEB-2022, entry version 58.
DE   RecName: Full=Holin-like protein 24.1 {ECO:0000312|EMBL:CAA66517.2};
DE   AltName: Full=Gene product 24.1 {ECO:0000305};
DE            Short=gp24.1 {ECO:0000305};
GN   Name=24.1;
OS   Bacillus phage SPP1 (Bacteriophage SPP1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=10724;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9434185; DOI=10.1016/s0378-1119(97)00547-7;
RA   Alonso J.C., Luder G., Stiege A.C., Chai S., Weise F., Trautner T.A.;
RT   "The complete nucleotide sequence and functional organization of Bacillus
RT   subtilis bacteriophage SPP1.";
RL   Gene 204:201-212(1997).
RN   [2]
RP   FUNCTION.
RX   PubMed=27328857; DOI=10.1111/mmi.13448;
RA   Fernandes S., Sao-Jose C.;
RT   "More than a hole: the holin lethal function may be required to fully
RT   sensitize bacteria to the lytic action of canonical endolysins.";
RL   Mol. Microbiol. 102:92-106(2016).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=27825035; DOI=10.1016/j.virol.2016.10.030;
RA   Fernandes S., Sao-Jose C.;
RT   "Probing the function of the two holin-like proteins of bacteriophage
RT   SPP1.";
RL   Virology 500:184-189(2017).
CC   -!- FUNCTION: Probably functions as a holin together with holin-like
CC       protein 26. Accumulates harmlessly in the cytoplasmic membrane until it
CC       reaches a critical concentration that triggers the formation of micron-
CC       scale pores (holes) causing host cell membrane disruption and endolysin
CC       escape into the periplasmic space. Determines the precise timing of
CC       host cell lysis. {ECO:0000305|PubMed:27328857}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane
CC       {ECO:0000269|PubMed:27825035}; Single-pass membrane protein
CC       {ECO:0000255}.
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DR   EMBL; X97918; CAA66517.2; -; Genomic_DNA.
DR   PIR; T42310; T42310.
DR   RefSeq; NP_690701.2; NC_004166.2.
DR   SMR; O48470; -.
DR   TCDB; 1.E.31.1.13; the spp1 holin (spp1 holin) family.
DR   GeneID; 955351; -.
DR   KEGG; vg:955351; -.
DR   Proteomes; UP000002559; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IDA:CACAO.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR019715; Haemolysin_XhlA.
DR   Pfam; PF10779; XhlA; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Viral release from host cell.
FT   CHAIN           1..83
FT                   /note="Holin-like protein 24.1"
FT                   /id="PRO_0000439630"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   COILED          1..45
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   83 AA;  9530 MW;  D2FB55E3D6A5A58A CRC64;
     MEQMRQDIIE LKQNDKSQEQ RISLLERTSD RHDQQIQAVT ESLSKIHENT TWIKRTITGA
     IISTLSTVVV GGILTIVWNL VKN