ID   HOL2_HOLDI              Reviewed;         127 AA.
AC   Q25054;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Holotricin-2;
DE   Flags: Precursor;
OS   Holotrichia diomphalia (Korean black chafer).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Scarabaeiformia;
OC   Scarabaeidae; Melolonthinae; Holotrichia.
OX   NCBI_TaxID=33394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 56-75 AND 82-126.
RC   TISSUE=Larval hemolymph;
RX   PubMed=8188641; DOI=10.1093/oxfordjournals.jbchem.a124309;
RA   Lee S.Y., Moon H.J., Kurata S., Kurama T., Natori S., Lee B.L.;
RT   "Purification and molecular cloning of cDNA for an inducible antibacterial
RT   protein of larvae of a coleopteran insect, Holotrichia diomphalia.";
RL   J. Biochem. 115:82-86(1994).
CC   -!- FUNCTION: Antibacterial activity against Gram-negative bacteria but not
CC       against Gram-positive bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Hemolymph.
CC   -!- SIMILARITY: Belongs to the coleoptericin family. {ECO:0000305}.
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DR   EMBL; D13745; BAA02890.1; -; mRNA.
DR   AlphaFoldDB; Q25054; -.
DR   SMR; Q25054; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR009382; Coleoptericin.
DR   Pfam; PF06286; Coleoptericin; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Immunity; Innate immunity; Secreted; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   PROPEP          16..55
FT                   /evidence="ECO:0000269|PubMed:8188641"
FT                   /id="PRO_0000004986"
FT   CHAIN           56..127
FT                   /note="Holotricin-2"
FT                   /id="PRO_0000004987"
FT   REGION          43..84
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        67..84
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   127 AA;  14075 MW;  3478B6DD308AC35B CRC64;
     MMKLVIALCL IGISAAYVVP VYYEIYPEDA TFDEADIEPQ LSPAELHHGS IRERRSLQPG
     APSFPMPGSQ LPTSVSGNVE KQGRNTIATI DAQHKTDRYD VRGTWTKVVD GPGRSKPNFR
     IGGSYRW