ID   HOL3_HOLDI              Reviewed;         104 AA.
AC   Q25055;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 2.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Holotricin-3;
DE   Flags: Precursor;
OS   Holotrichia diomphalia (Korean black chafer).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Scarabaeiformia;
OC   Scarabaeidae; Melolonthinae; Holotrichia.
OX   NCBI_TaxID=33394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 21-40.
RC   TISSUE=Larval hemolymph;
RX   PubMed=8535393; DOI=10.1248/bpb.18.1049;
RA   Lee S.Y., Moon H.J., Kurata S., Natori S., Lee B.L.;
RT   "Purification and cDNA cloning of an antifungal protein from the hemolymph
RT   of Holotrichia diomphalia larvae.";
RL   Biol. Pharm. Bull. 18:1049-1052(1995).
CC   -!- FUNCTION: Has antifungal activity against C.albicans.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: To T.molitor tenecin 3. {ECO:0000305}.
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DR   EMBL; D13744; BAA02889.1; -; mRNA.
DR   PIR; JC4190; JC4190.
DR   AlphaFoldDB; Q25055; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Immunity;
KW   Innate immunity; Repeat; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:8535393"
FT   CHAIN           21..104
FT                   /note="Holotricin-3"
FT                   /id="PRO_0000004990"
FT   REPEAT          27..30
FT                   /note="1"
FT   REPEAT          31..34
FT                   /note="2"
FT   REPEAT          35..38
FT                   /note="3"
FT   REPEAT          39..42
FT                   /note="4"
FT   REPEAT          43..46
FT                   /note="5"
FT   REPEAT          47..50
FT                   /note="6"
FT   REPEAT          51..54
FT                   /note="7"
FT   REPEAT          55..58
FT                   /note="8"
FT   REPEAT          59..62
FT                   /note="9"
FT   REPEAT          63..66
FT                   /note="10"
FT   REPEAT          67..70
FT                   /note="11"
FT   REPEAT          71..74
FT                   /note="12"
FT   REPEAT          75..78
FT                   /note="13"
FT   REPEAT          79..82
FT                   /note="14"
FT   REPEAT          83..86
FT                   /note="15"
FT   REPEAT          87..90
FT                   /note="16"
FT   REPEAT          91..94
FT                   /note="17"
FT   REPEAT          96..98
FT                   /note="18"
FT   REGION          22..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          27..98
FT                   /note="18 X 4 AA approximate tandem repeats of H-G-G-G"
SQ   SEQUENCE   104 AA;  9026 MW;  2799D681BFDCC725 CRC64;
     MNKLIILGLA CIIAVASAMP YGPGDGHGGG HGGGHGGGHG NGQGGGHGHG PGGGFGGGHG
     GGHGGGGRGG GGSGGGGSPG HGAGGGYPGG HGGGHHGGYQ THGY