HOLB_PSEAE
ID HOLB_PSEAE Reviewed; 328 AA.
AC P52024;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=DNA polymerase III subunit delta';
DE EC=2.7.7.7;
GN Name=holB; OrderedLocusNames=PA2961;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8550441; DOI=10.1128/jb.178.1.46-53.1996;
RA Alm R.A., Bodero A.J., Free P.D., Mattick J.S.;
RT "Identification of a novel gene, pilZ, essential for type 4 fimbrial
RT biogenesis in Pseudomonas aeruginosa.";
RL J. Bacteriol. 178:46-53(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC responsible for most of the replicative synthesis in bacteria. This DNA
CC polymerase also exhibits 3' to 5' exonuclease activity (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC and theta chains) that associates with a tau subunit. This core
CC dimerizes to form the POLIII' complex. PolIII' associates with the
CC gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC and with the beta chain to form the complete DNA polymerase III complex
CC (By similarity). {ECO:0000250}.
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DR EMBL; L42622; AAA93518.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06349.1; -; Genomic_DNA.
DR PIR; A59241; A59241.
DR PIR; D83275; D83275.
DR RefSeq; NP_251651.1; NC_002516.2.
DR RefSeq; WP_003104126.1; NZ_QZGE01000009.1.
DR AlphaFoldDB; P52024; -.
DR SMR; P52024; -.
DR STRING; 287.DR97_4978; -.
DR PaxDb; P52024; -.
DR PRIDE; P52024; -.
DR EnsemblBacteria; AAG06349; AAG06349; PA2961.
DR GeneID; 880379; -.
DR KEGG; pae:PA2961; -.
DR PATRIC; fig|208964.12.peg.3107; -.
DR PseudoCAP; PA2961; -.
DR HOGENOM; CLU_006229_4_3_6; -.
DR InParanoid; P52024; -.
DR OMA; DWFCDWA; -.
DR PhylomeDB; P52024; -.
DR BioCyc; PAER208964:G1FZ6-3013-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009360; C:DNA polymerase III complex; IBA:GO_Central.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR004622; DNA_pol_HolB.
DR InterPro; IPR015199; DNA_pol_III_delta_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF09115; DNApol3-delta_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00678; holB; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-directed DNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transferase.
FT CHAIN 1..328
FT /note="DNA polymerase III subunit delta'"
FT /id="PRO_0000105515"
FT CONFLICT 16..30
FT /note="GGRAQHAHAYLLYGP -> RPRPARPRLSALAS (in Ref. 1;
FT AAA93518)"
FT /evidence="ECO:0000305"
FT CONFLICT 48..52
FT /note="CQRPA -> TRAC (in Ref. 1; AAA93518)"
FT /evidence="ECO:0000305"
FT CONFLICT 176..177
FT /note="RA -> PG (in Ref. 1; AAA93518)"
FT /evidence="ECO:0000305"
FT CONFLICT 208..209
FT /note="VR -> AG (in Ref. 1; AAA93518)"
FT /evidence="ECO:0000305"
FT CONFLICT 238..251
FT /note="SVPLPLLFDWFCDW -> RVPIAIALRLVLRL (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 285..286
FT /note="QA -> HR (in Ref. 1; AAA93518)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="A -> G (in Ref. 1; AAA93518)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 328 AA; 35746 MW; 55DAA4F223C1464D CRC64;
MADIYPWQQA LWSQLGGRAQ HAHAYLLYGP AGIGKRALAE HWAAQLLCQR PAAAGACGEC
KACQLLAAGT HPDYFVLEPE EAEKPIRVDQ VRDLVGFVVQ TAQLGGRKVV LLEPAEAMNV
NAANALLKSL EEPSGDTVLL LISHQPSRLL PTIKSRCVQQ ACPLPGAAAS LEWLARALPD
EPAEALEELL ALSGGSPLTA QRLHGQGVRE QRAQVVEGVK KLLKQQIAAS PLAESWNSVP
LPLLFDWFCD WTLGILRYQL THDEEGLGLA DMRKVIQYLG DKSGQAKVLA MQDWLLQQRQ
KVLNKANLNR VLLLEALLVQ WASLPGPG