ID   HOLIN_BPDP1             Reviewed;          74 AA.
AC   O03978;
DT   27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   12-AUG-2020, entry version 55.
DE   RecName: Full=Holin;
GN   Name=dph;
OS   Pneumococcus phage Dp-1 (Bacteriophage Dp-1).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=59241;
OH   NCBI_TaxID=1313; Streptococcus pneumoniae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9379901; DOI=10.1046/j.1365-2958.1997.5101880.x;
RA   Sheehan M.M., Garcia J.L., Lopez R., Garcia P.;
RT   "The lytic enzyme of the pneumococcal phage Dp-1: a chimeric lysin of
RT   intergeneric origin.";
RL   Mol. Microbiol. 25:717-725(1997).
CC   -!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until it
CC       reaches a critical concentration that triggers the formation of micron-
CC       scale pores (holes) causing host cell membrane disruption and endolysin
CC       escape into the periplasmic space. Determines the precise timing of
CC       host cell lysis. Participates with the endolysin protein in the
CC       sequential events which lead to the programmed host cell lysis
CC       releasing the mature viral particles from the host cell.
CC       {ECO:0000250|UniProtKB:O64204}.
CC   -!- SUBUNIT: Homomultimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; Z93946; CAB07985.1; -; Genomic_DNA.
DR   RefSeq; YP_004306946.1; NC_015274.1.
DR   TCDB; 1.E.24.1.1; the bacterophage dp-1 holin (dp-1 holin) family.
DR   GeneID; 10358654; -.
DR   KEGG; vg:10358654; -.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR031612; Phage_holin_Dp1.
DR   Pfam; PF16938; Phage_holin_Dp1; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Viral release from host cell.
FT   CHAIN           1..74
FT                   /note="Holin"
FT                   /id="PRO_0000077800"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        37..57
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   74 AA;  7861 MW;  0F6A3884A8437DE6 CRC64;
     MKLSNEQYDV AKNVVTVVVP AAIALITGLG ALYQFDTTAI TGTIALLATF AGTVLGVSSR
     NYQKEQEAQN NEVE