ID   HOLIN_BPKMV             Reviewed;          66 AA.
AC   Q7Y2C1;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   12-AUG-2020, entry version 54.
DE   RecName: Full=Holin {ECO:0000305};
DE   AltName: Full=KMV44 {ECO:0000303|PubMed:21687532};
DE   AltName: Full=Pinholin {ECO:0000303|PubMed:21687532};
GN   ORFNames=44;
OS   Pseudomonas phage phiKMV.
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus.
OX   NCBI_TaxID=204270;
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12890620; DOI=10.1016/s0042-6822(03)00123-5;
RA   Lavigne R., Burkal'tseva M.V., Robben J., Sykilinda N.N., Kurochkina L.P.,
RA   Grymonprez B., Jonckx B., Krylov V.N., Mesyanzhinov V.V., Volckaert G.;
RT   "The genome of bacteriophage phiKMV, a T7-like virus infecting Pseudomonas
RT   aeruginosa.";
RL   Virology 312:49-59(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15549178; DOI=10.1007/s00018-004-4301-y;
RA   Lavigne R., Briers Y., Hertveldt K., Robben J., Volckaert G.;
RT   "Identification and characterization of a highly thermostable bacteriophage
RT   lysozyme.";
RL   Cell. Mol. Life Sci. 61:2753-2759(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16522177; DOI=10.2174/0929866054696127;
RA   Lavigne R., Roucourt B., Hertveldt K., Volckaert G.;
RT   "Characterization of the bacteriophage PhiKMV DNA ligase.";
RL   Protein Pept. Lett. 12:645-648(2005).
RN   [4]
RP   CHARACTERIZATION, AND FUNCTION.
RX   PubMed=21687532; DOI=10.4161/bact.1.1.14868;
RA   Briers Y., Peeters L.M., Volckaert G., Lavigne R.;
RT   "The lysis cassette of bacteriophage varphiKMV encodes a signal-arrest-
RT   release endolysin and a pinholin.";
RL   Bacteriophage 1:25-30(2011).
CC   -!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until it
CC       reaches a critical concentration that triggers the formation of
CC       nanometer-scale pores (pinholes) causing host cell membrane
CC       depolarization and endolysin refolding and release into the periplasmic
CC       space (PubMed:21687532). Once the pinholin has permeabilized the host
CC       cell membrane, the SAR-endolysin is released into the periplasm and
CC       breaks down the peptidoglycan layer (PubMed:21687532). Determines the
CC       precise timing of host cell lysis. Participates with the SAR-endolysin
CC       and the U-spanin protein in the sequential events which lead to the
CC       programmed host cell lysis releasing the mature viral particles from
CC       the host cell (PubMed:21687532). {ECO:0000269|PubMed:21687532}.
CC   -!- SUBUNIT: Homomultimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane
CC       {ECO:0000250|UniProtKB:P03705}; Single-pass type II membrane protein
CC       {ECO:0000255}.
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DR   EMBL; AJ505558; CAD44235.1; -; Genomic_DNA.
DR   RefSeq; NP_877483.1; NC_005045.1.
DR   SMR; Q7Y2C1; -.
DR   TCDB; 1.E.61.1.1; the pseudomonas phage phikmv (ppkmv holin) family.
DR   GeneID; 2641773; -.
DR   KEGG; vg:2641773; -.
DR   Proteomes; UP000000842; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0034292; F:pinholin activity; IGI:CACAO.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral release from host cell.
FT   CHAIN           1..66
FT                   /note="Holin"
FT                   /id="PRO_0000429258"
FT   TOPO_DOM        1..29
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        51..66
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   66 AA;  7016 MW;  50061520107492EB CRC64;
     MMLDTATEAG KGTLAVTGVG IAVYSPYEIA SLCAAVLTAL YVGAQLITLL PKMLDSIAEL
     RRRFKK