ID   AOR_PYRHO               Reviewed;         607 AA.
AC   O58778;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=Tungsten-containing aldehyde ferredoxin oxidoreductase;
DE            EC=1.2.7.5;
GN   Name=aor; OrderedLocusNames=PH1019;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = a
CC         carboxylate + 3 H(+) + 2 reduced [2Fe-2S]-[ferredoxin];
CC         Xref=Rhea:RHEA:16421, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17478,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.2.7.5;
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=W-bis(molybdopterin guanine dinucleotide); Xref=ChEBI:CHEBI:60537;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 W-bis(molybdopterin guanine dinucleotide) (W-bis-MGD)
CC       cofactor per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the AOR/FOR family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000001; BAA30116.1; -; Genomic_DNA.
DR   PIR; F71094; F71094.
DR   RefSeq; WP_010885106.1; NC_000961.1.
DR   AlphaFoldDB; O58778; -.
DR   SMR; O58778; -.
DR   STRING; 70601.3257433; -.
DR   EnsemblBacteria; BAA30116; BAA30116; BAA30116.
DR   GeneID; 1443341; -.
DR   KEGG; pho:PH1019; -.
DR   eggNOG; arCOG00706; Archaea.
DR   OMA; IHIGQFR; -.
DR   OrthoDB; 9593at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0033726; F:aldehyde ferredoxin oxidoreductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009055; F:electron transfer activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.569.10; -; 1.
DR   Gene3D; 1.10.599.10; -; 1.
DR   Gene3D; 3.60.9.10; -; 1.
DR   InterPro; IPR013984; Ald_Fedxn_OxRdtase_dom2.
DR   InterPro; IPR013985; Ald_Fedxn_OxRdtase_dom3.
DR   InterPro; IPR013983; Ald_Fedxn_OxRdtase_N.
DR   InterPro; IPR036503; Ald_Fedxn_OxRdtase_N_sf.
DR   InterPro; IPR001203; OxRdtase_Ald_Fedxn_C.
DR   InterPro; IPR036021; Tungsten_al_ferr_oxy-like_C.
DR   Pfam; PF01314; AFOR_C; 1.
DR   Pfam; PF02730; AFOR_N; 1.
DR   SMART; SM00790; AFOR_N; 1.
DR   SUPFAM; SSF48310; SSF48310; 1.
DR   SUPFAM; SSF56228; SSF56228; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Tungsten.
FT   CHAIN           1..607
FT                   /note="Tungsten-containing aldehyde ferredoxin
FT                   oxidoreductase"
FT                   /id="PRO_0000064607"
SQ   SEQUENCE   607 AA;  67537 MW;  978039536AA58FBE CRC64;
     MFGYWGKILR VNLSDGTIKE ETFNEEFAKK WLGTRGFGIY FLLKEMDPKI DPFSPENKLI
     FATGPLTGTS APTGGRYMVI TKSPLTGYIA MANSGGYFGA ELKFAGWDAI IVEGKADHPV
     YIYINDENVE IRDASKVWGK LVSETERMLK EEVGDKHVQI ASIGPAGENK VRFAAVMNNG
     HRAAGRGGVG AVMGSKNLKA IVVRGHKRVE VADKGKFMEV IREKIEKLRK DPVAGGGLPK
     YGTAVLVNII NEHGLYPTRN FQTGVFKYAY EQSGEAMAAK YLVRNKPCFA CPIGCGRVNY
     LPSIGETEGP EYESTWALGA NLGINDLASI IEANHFADEY GMDTISLGGT LATAMELYER
     GLIKQEDIGG ENEPPFRFGN TEVLHYWIHK IAKREGFGDI LAEGGYRLAE KFNGLEYFMG
     VKKQELPAYD PRGAEGHGLG YATNNRGGCH IKQYMISPEI LGYPYKMDPH DISDEKVKMV
     ILFQDLTAVI DAAGLCIFTT FGLGADDYTD MLNAALGWDF STDDYLKIGE RIWNAERLFN
     LKAGLDPLKE DTLPKRFLEE PMPEGPNKGH VVRLKEMLPR YYKLRGWTED GRIPEEKLKE
     LGLEEFM