ID   HOLIN_BPR1T             Reviewed;          75 AA.
AC   Q38134;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   12-AUG-2020, entry version 65.
DE   RecName: Full=Holin;
OS   Lactococcus phage r1t (Bacteriophage r1t).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae.
OX   NCBI_TaxID=43685;
OH   NCBI_TaxID=1359; Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8730875; DOI=10.1111/j.1365-2958.1996.tb02478.x;
RA   van Sinderen D., Karsens H., Kok J., Terpstra P., Ruiters M.H., Venema G.,
RA   Nauta A.;
RT   "Sequence analysis and molecular characterization of the temperate
RT   lactococcal bacteriophage r1t.";
RL   Mol. Microbiol. 19:1343-1355(1996).
CC   -!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until it
CC       reaches a critical concentration that triggers the formation of micron-
CC       scale pores (holes) causing host cell membrane disruption and endolysin
CC       escape into the periplasmic space. Determines the precise timing of
CC       host cell lysis. Participates with the endolysin protein in the
CC       sequential events which lead to the programmed host cell lysis
CC       releasing the mature viral particles from the host cell.
CC       {ECO:0000250|UniProtKB:O64204}.
CC   -!- SUBUNIT: Homomultimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; U38906; AAB18723.1; -; Genomic_DNA.
DR   RefSeq; NP_695076.1; NC_004302.1.
DR   TCDB; 1.E.18.1.1; the lactococcus lactis phage r1t holin (r1t holin) family.
DR   GeneID; 955473; -.
DR   KEGG; vg:955473; -.
DR   Proteomes; UP000001164; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR020109; Holin_r1t.
DR   Pfam; PF16945; Phage_r1t_holin; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Viral release from host cell.
FT   CHAIN           1..75
FT                   /note="Holin"
FT                   /id="PRO_0000077801"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   75 AA;  7688 MW;  3E71E8458B47D0C4 CRC64;
     MKTFFKDMAE RAIKTFAQAM IGALGAGATG LIGVDWLQAL SIAGFATVVS ILTSLASGIP
     GDNTASLVNN KKEGE