ID   HOLIN_BPT3              Reviewed;          67 AA.
AC   P10307;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   07-OCT-2020, entry version 44.
DE   RecName: Full=Holin {ECO:0000255|HAMAP-Rule:MF_04108};
DE   AltName: Full=Lysis protein;
GN   Name=17.5; Synonyms=lys;
OS   Enterobacteria phage T3 (Bacteriophage T3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus;
OC   Escherichia virus T3.
OX   NCBI_TaxID=10759;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3010556; DOI=10.1016/0042-6822(86)90055-3;
RA   Yamada M., Fujisawa H., Kato H., Hamada K., Minagawa T.;
RT   "Cloning and sequencing of the genetic right end of bacteriophage T3 DNA.";
RL   Virology 151:350-361(1986).
RN   [2]
RP   ERRATUM OF PUBMED:3010556.
RA   Yamada M., Fujisawa H., Kato H., Hamada K., Minagawa T.;
RL   Virology 154:246-246(1986).
RN   [3]
RP   FUNCTION.
RX   PubMed=685184; DOI=10.1016/0042-6822(78)90067-3;
RA   Miyazaki J.I., Ryo Y., Fujisawa H., Minagawa T.;
RT   "Mutation in bacteriophage T3 affecting host cell lysis.";
RL   Virology 89:327-329(1978).
RN   [4]
RP   REVIEW.
RX   PubMed=11018145; DOI=10.1146/annurev.micro.54.1.799;
RA   Wang I.N., Smith D.L., Young R.;
RT   "Holins: the protein clocks of bacteriophage infections.";
RL   Annu. Rev. Microbiol. 54:799-825(2000).
CC   -!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until it
CC       reaches a critical concentration that triggers the formation of micron-
CC       scale pores (holes) causing host cell membrane disruption and endolysin
CC       escape into the periplasmic space. Participates in determining the
CC       precise timing of host cell lysis. Participates with the endolysin and
CC       spanin proteins in the sequential events which lead to the programmed
CC       host cell lysis releasing the mature viral particles from the host
CC       cell. {ECO:0000255|HAMAP-Rule:MF_04108, ECO:0000305|PubMed:11018145,
CC       ECO:0000305|PubMed:685184}.
CC   -!- SUBUNIT: Homomultimer. {ECO:0000255|HAMAP-Rule:MF_04108,
CC       ECO:0000305|PubMed:11018145}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04108, ECO:0000305|PubMed:11018145}; Single-pass type II
CC       membrane protein {ECO:0000255|HAMAP-Rule:MF_04108,
CC       ECO:0000305|PubMed:11018145}; Periplasmic side {ECO:0000255|HAMAP-
CC       Rule:MF_04108, ECO:0000305|PubMed:11018145}. Note=Classified as a class
CC       II holin although it seems to have only one transmembrane domain.
CC       {ECO:0000255|HAMAP-Rule:MF_04108, ECO:0000303|PubMed:11018145}.
CC   -!- SIMILARITY: Belongs to the T7likevirus holin family.
CC       {ECO:0000255|HAMAP-Rule:MF_04108}.
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DR   EMBL; M14784; AAA92524.1; ALT_TERM; Genomic_DNA.
DR   PIR; B23476; Q7BPT3.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034291; F:canonical holin activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04108; HOLIN_T7; 1.
DR   InterPro; IPR019682; Phage_T7_Gp17.5_holin.
DR   Pfam; PF10746; Phage_holin_2_2; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Viral release from host cell.
FT   CHAIN           1..67
FT                   /note="Holin"
FT                   /id="PRO_0000106532"
FT   TOPO_DOM        1..36
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04108"
FT   TRANSMEM        37..55
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04108"
FT   TOPO_DOM        56..67
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04108"
SQ   SEQUENCE   67 AA;  7472 MW;  E751BDC9883F4AD5 CRC64;
     MLSLDFNNEL IKAAPIVGTG VADVSARRFF GLSLNEWFYV ARIAYTVVXI GAKVVDKIID
     WKKATKE