HOLIN_BPT5
ID HOLIN_BPT5 Reviewed; 227 AA.
AC Q6R6U4; Q66M09; Q6QGP6;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 42.
DE RecName: Full=Holin {ECO:0000255|HAMAP-Rule:MF_04104, ECO:0000303|PubMed:24198424};
GN Name=C1 {ECO:0000312|EMBL:AAS77088.1};
GN ORFNames=orf11c {ECO:0000312|EMBL:AAS19394.1},
GN T5.041 {ECO:0000312|EMBL:AAS77088.1}, T5p040 {ECO:0000312|EMBL:AAU05193.1};
OS Escherichia phage T5 (Enterobacteria phage T5).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX NCBI_TaxID=2695836;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
RX PubMed=14711503; DOI=10.1016/j.pep.2003.07.006;
RA Mikoulinskaia G.V., Zimin A.A., Feofanov S.A., Miroshnikov A.I.;
RT "Identification, cloning, and expression of bacteriophage T5 dnk gene
RT encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC
RT 2.7.4.13).";
RL Protein Expr. Purif. 33:166-175(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT "Bacteriophage T5 complete genome.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX11974.1};
RX PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA Hu S.;
RT "Complete genome sequence of bacteriophage T5.";
RL Virology 332:45-65(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=St0 deletion mutant;
RX PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA Boulanger P.;
RT "Insights into bacteriophage T5 structure from analysis of its
RT morphogenesis genes and protein components.";
RL J. Virol. 88:1162-1174(2014).
CC -!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until it
CC reaches a critical concentration that triggers the formation of micron-
CC scale pores (holes) causing host cell membrane disruption and endolysin
CC escape into the periplasmic space. Determines the precise timing of
CC host cell lysis. Participates with the endolysin and spanin proteins in
CC the sequential events which lead to the programmed host cell lysis
CC releasing the mature viral particles from the host cell.
CC {ECO:0000255|HAMAP-Rule:MF_04104}.
CC -!- SUBUNIT: Homomultimer. Interacts (via C-terminus) with antiholin (via
CC C-terminus); this interaction blocks the holin homomultimerization and
CC delays host cell lysis. Interacts (via N-terminus) with the lysis
CC inhibition accessory protein rIII; this interaction stabilizes the
CC holin-antiholin complex thereby resulting in a robust block of the hole
CC formation. {ECO:0000255|HAMAP-Rule:MF_04104}.
CC -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_04104}; Single-pass type II membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_04104}; Periplasmic side {ECO:0000255|HAMAP-
CC Rule:MF_04104}. Note=Classified as a class III holin.
CC {ECO:0000255|HAMAP-Rule:MF_04104}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1;
CC IsoId=Q6R6U4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6R6U4-2; Sequence=VSP_058112;
CC -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC {ECO:0000305|PubMed:15661140}.
CC -!- PTM: Disulfide bond is required for functionality. {ECO:0000255|HAMAP-
CC Rule:MF_04104}.
CC -!- SIMILARITY: Belongs to the T4likevirus holin family.
CC {ECO:0000255|HAMAP-Rule:MF_04104}.
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DR EMBL; AY509815; AAS19388.1; -; Genomic_DNA.
DR EMBL; AY509815; AAS19394.1; -; Genomic_DNA.
DR EMBL; AY692264; AAU05193.1; -; Genomic_DNA.
DR EMBL; AY543070; AAS77088.1; -; Genomic_DNA.
DR EMBL; AY587007; AAX11974.1; -; Genomic_DNA.
DR RefSeq; YP_006869.1; NC_005859.1. [Q6R6U4-2]
DR SMR; Q6R6U4; -.
DR TCDB; 1.E.8.1.4; the t4 holin (t4 holin) family.
DR GeneID; 2777575; -.
DR KEGG; vg:2777575; -.
DR Proteomes; UP000002107; Genome.
DR Proteomes; UP000002141; Genome.
DR Proteomes; UP000002503; Genome.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0034291; F:canonical holin activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR HAMAP; MF_04104; HOLIN_T4; 1.
DR InterPro; IPR020982; Phage_T4_GpT_holin.
DR Pfam; PF11031; Phage_holin_T; 1.
PE 2: Evidence at transcript level;
KW Alternative initiation; Cytolysis; Disulfide bond;
KW Host cell inner membrane; Host cell lysis by virus; Host cell membrane;
KW Host membrane; Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix; Viral release from host cell.
FT CHAIN 1..227
FT /note="Holin"
FT /id="PRO_0000435546"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04104"
FT TRANSMEM 34..48
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04104"
FT TOPO_DOM 49..227
FT /note="Periplasmic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04104"
FT DISULFID 178..216
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04104"
FT VAR_SEQ 1..10
FT /note="MVLVRGGYKL -> M (in isoform 2)"
FT /id="VSP_058112"
SQ SEQUENCE 227 AA; 25869 MW; 1C6F0296CBEF1752 CRC64;
MVLVRGGYKL EKFLQLLTVL LQEAKDPASL LKRLLTILVA VIIFLFVSNT SEVMSFLKTF
STSAVLQDVQ TQRIDNFPNV AREKSMVLFS QTGADAVFVV KYKPDAINDY SNIIAWESNA
QLDRADLADK AVNKTSELYR RHLEGFNYAS DLTVKVNKYM GKNIPSFKNV IFNYIYTCPY
FNLNNIYAGY IGIAWRDKPV DIADSEQFKE YLTKLCSPQQ RSLGRSI
