ID   HOLIN_BPT7              Reviewed;          67 AA.
AC   P03802;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 69.
DE   RecName: Full=Holin {ECO:0000255|HAMAP-Rule:MF_04108};
DE   AltName: Full=Gene product 17.5;
DE            Short=Gp17.5;
GN   OrderedLocusNames=17.5;
OS   Escherichia phage T7 (Bacteriophage T7).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus.
OX   NCBI_TaxID=10760;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6864790; DOI=10.1016/s0022-2836(83)80282-4;
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of
RT   T7 genetic elements.";
RL   J. Mol. Biol. 166:477-535(1983).
RN   [2]
RP   REVIEW.
RX   PubMed=11018145; DOI=10.1146/annurev.micro.54.1.799;
RA   Wang I.N., Smith D.L., Young R.;
RT   "Holins: the protein clocks of bacteriophage infections.";
RL   Annu. Rev. Microbiol. 54:799-825(2000).
RN   [3]
RP   FUNCTION.
RX   PubMed=24335287; DOI=10.1128/jvi.03274-13;
RA   Nguyen H.M., Kang C.;
RT   "Lysis delay and burst shrinkage of coliphage T7 by deletion of terminator
RT   Tphi reversed by deletion of early genes.";
RL   J. Virol. 88:2107-2115(2014).
CC   -!- FUNCTION: Accumulates harmlessly in the cytoplasmic membrane until it
CC       reaches a critical concentration that triggers the formation of micron-
CC       scale pores (holes) causing host cell membrane disruption and endolysin
CC       escape into the periplasmic space. Participates in determining the
CC       precise timing of host cell lysis. Participates with the endolysin and
CC       spanin proteins in the sequential events which lead to the programmed
CC       host cell lysis releasing the mature viral particles from the host
CC       cell. {ECO:0000255|HAMAP-Rule:MF_04108, ECO:0000305|PubMed:11018145,
CC       ECO:0000305|PubMed:24335287}.
CC   -!- SUBUNIT: Homomultimer. {ECO:0000255|HAMAP-Rule:MF_04108,
CC       ECO:0000305|PubMed:11018145}.
CC   -!- SUBCELLULAR LOCATION: Host cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04108, ECO:0000305|PubMed:11018145}; Single-pass type II
CC       membrane protein {ECO:0000255|HAMAP-Rule:MF_04108,
CC       ECO:0000305|PubMed:11018145}; Periplasmic side {ECO:0000255|HAMAP-
CC       Rule:MF_04108, ECO:0000305|PubMed:11018145}. Note=Classified as a class
CC       II holin although it seems to have only one transmembrane domain.
CC       {ECO:0000255|HAMAP-Rule:MF_04108, ECO:0000303|PubMed:11018145}.
CC   -!- SIMILARITY: Belongs to the T7likevirus holin family.
CC       {ECO:0000255|HAMAP-Rule:MF_04108}.
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DR   EMBL; V01146; CAA24436.1; -; Genomic_DNA.
DR   PIR; A04427; Q7BPE7.
DR   RefSeq; NP_042006.1; NC_001604.1.
DR   TCDB; 1.E.6.1.1; the t7 holin (t7 holin) family.
DR   GeneID; 1261022; -.
DR   KEGG; vg:1261022; -.
DR   Proteomes; UP000000840; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0034291; F:canonical holin activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04108; HOLIN_T7; 1.
DR   InterPro; IPR019682; Phage_T7_Gp17.5_holin.
DR   Pfam; PF10746; Phage_holin_2_2; 1.
PE   3: Inferred from homology;
KW   Cytolysis; Host cell inner membrane; Host cell lysis by virus;
KW   Host cell membrane; Host membrane; Membrane; Reference proteome;
KW   Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral release from host cell.
FT   CHAIN           1..67
FT                   /note="Holin"
FT                   /id="PRO_0000106533"
FT   TOPO_DOM        1..36
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04108"
FT   TRANSMEM        37..55
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04108"
FT   TOPO_DOM        56..67
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04108"
SQ   SEQUENCE   67 AA;  7392 MW;  FEA1040A783F4AC7 CRC64;
     MLSLDFNNEL IKAAPIVGTG VADVSARLFF GLSLNEWFYV AAIAYTVVQI GAKVVDKMID
     WKKANKE