ID   HOOK_CULQU              Reviewed;         691 AA.
AC   B0WPU9;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Protein hook;
GN   Name=hook {ECO:0000250|UniProtKB:Q24185};
GN   Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=CPIJ009268;
OS   Culex quinquefasciatus (Southern house mosquito) (Culex pungens).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7176;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JHB;
RG   The Broad Institute Genome Sequencing Platform;
RA   Atkinson P.W., Hemingway J., Christensen B.M., Higgs S., Kodira C.D.,
RA   Hannick L.I., Megy K., O'Leary S.B., Pearson M., Haas B.J., Mauceli E.,
RA   Wortman J.R., Lee N.H., Guigo R., Stanke M., Alvarado L., Amedeo P.,
RA   Antoine C.H., Arensburger P., Bidwell S.L., Crawford M., Camaro F.,
RA   Devon K., Engels R., Hammond M., Howarth C., Koehrsen M., Lawson D.,
RA   Montgomery P., Nene V., Nusbaum C., Puiu D., Romero-Severson J.,
RA   Severson D.W., Shumway M., Sisk P., Stolte C., Zeng Q., Eisenstadt E.,
RA   Fraser-Liggett C.M., Strausberg R., Galagan J., Birren B., Collins F.H.;
RT   "Annotation of Culex pipiens quinquefasciatus.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in endocytic trafficking. Probably acts as a
CC       cytoskeletal linker protein that tethers endosome vesicles to the
CC       cytoskeleton. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q24185}. Endosome
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDS32537.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; DS232030; EDS32537.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001850733.1; XM_001850681.1.
DR   AlphaFoldDB; B0WPU9; -.
DR   SMR; B0WPU9; -.
DR   STRING; 7176.CPIJ009268-PA; -.
DR   PRIDE; B0WPU9; -.
DR   GeneID; 6041481; -.
DR   KEGG; cqu:CpipJ_CPIJ009268; -.
DR   VEuPathDB; VectorBase:CPIJ009268; -.
DR   VEuPathDB; VectorBase:CQUJHB002451; -.
DR   eggNOG; ENOG502QQM8; Eukaryota.
DR   HOGENOM; CLU_011214_1_0_1; -.
DR   InParanoid; B0WPU9; -.
DR   OrthoDB; 398210at2759; -.
DR   Proteomes; UP000002320; Partially assembled WGS sequence.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR036872; CH_dom_sf.
DR   InterPro; IPR008636; Hook_C.
DR   InterPro; IPR043936; HOOK_N.
DR   InterPro; IPR038077; Troponin_sf.
DR   Pfam; PF05622; HOOK; 1.
DR   Pfam; PF19047; HOOK_N; 1.
DR   SUPFAM; SSF90250; SSF90250; 1.
DR   PROSITE; PS50021; CH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Endocytosis; Endosome; Microtubule;
KW   Reference proteome.
FT   CHAIN           1..691
FT                   /note="Protein hook"
FT                   /id="PRO_0000379061"
FT   DOMAIN          8..124
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   REGION          431..454
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          595..633
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          667..691
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          136..568
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        602..633
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   691 AA;  77998 MW;  31D482BE30A71F6C CRC64;
     MDSCQICMEI YESLIRWLRV LQLSAPHDNE KDLSDGVALA QALNQIAPEM FSDSWLSKIK
     SDVGHNWRLK VSNLKKIIEG VYVYYQDILS LNLAEELRPD AMKIAEKCDG FELGRLLQLI
     LGCAVNCLEK QKYITQIMEL EESLQRNIMA ALQDIEYVWQ GASPSRNSIN APSLDVKTLQ
     EDRDSLAQKC HETNKKMLIL IEEKSVLQQE ISKLQVIIER YEHPNLIGDD GTSLGPIQLG
     SSRYNDLRKV VDGLKDELLQ AETARDDLKM KSAMQEKEIS DLQVKIDELH SATAEIAQLK
     DEIDILKEAN DKLKICETQL QTYKKKLEDY NDLKKQIKML EERSAEYLKQ NSDFEEEAKK
     YAGLKGQVEL YKKEIQDLHA KLDSEMGKTM KTEFEHNQLQ AKLSAVQREK ENLLSERDAL
     REACDELKCG QAAEDSESGR NTMSKELHSN DVRERIERLE RENKALREGQ GGQTALSQLL
     DDANQRNDKL RDQLKSANQR IMLLSQNHSD EISSKSDMDI QLKQALELNE QRSSQIDEAQ
     SQMGQLQAKI ANLESALVAK EQDLLAADTR YKKCVEKAKE VIKTLDPRAV NEALLLEKPP
     QQDGSEGSST TGGGDASTLT AASASSSSAR APMGVQEEQL IATAFYRLGM TCQREAVDSR
     LALLSGPGQS FLSRQRQPAP RKPMNMSYAK K