ID   HOOK_DROAN              Reviewed;         681 AA.
AC   B3MNR6;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Protein hook;
GN   Name=hook {ECO:0000250|UniProtKB:Q24185};
GN   Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GF15219;
OS   Drosophila ananassae (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14024-0371.13;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC       of the endocytic pathway. Probably acts as a cytoskeletal linker
CC       protein required to tether endosome vesicles to the cytoskeleton.
CC       Involved in modulation of endocytosis at stages required for down-
CC       regulation of membrane proteins that control synapse size. Not involved
CC       in synaptic vesicle recycling. Required in R7 cells for boss
CC       endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC       adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q24185}. Endosome
CC       {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC       Note=Enriched at neuromuscular synapses, in both presynaptic and
CC       postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
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DR   EMBL; CH902620; EDV31153.1; -; Genomic_DNA.
DR   RefSeq; XP_001961932.1; XM_001961896.2.
DR   AlphaFoldDB; B3MNR6; -.
DR   SMR; B3MNR6; -.
DR   STRING; 7217.FBpp0118411; -.
DR   EnsemblMetazoa; FBtr0119919; FBpp0118411; FBgn0092244.
DR   GeneID; 6498032; -.
DR   KEGG; dan:6498032; -.
DR   eggNOG; ENOG502QQM8; Eukaryota.
DR   HOGENOM; CLU_011214_1_0_1; -.
DR   InParanoid; B3MNR6; -.
DR   OMA; LEFNNHA; -.
DR   OrthoDB; 398210at2759; -.
DR   PhylomeDB; B3MNR6; -.
DR   Proteomes; UP000007801; Unassembled WGS sequence.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0031267; F:small GTPase binding; IEA:EnsemblMetazoa.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR   GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR036872; CH_dom_sf.
DR   InterPro; IPR008636; Hook_C.
DR   InterPro; IPR043936; HOOK_N.
DR   Pfam; PF05622; HOOK; 1.
DR   Pfam; PF19047; HOOK_N; 1.
DR   PROSITE; PS50021; CH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW   Endosome; Microtubule; Reference proteome; Synapse.
FT   CHAIN           1..681
FT                   /note="Protein hook"
FT                   /id="PRO_0000379062"
FT   DOMAIN          6..123
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   COILED          135..439
FT                   /evidence="ECO:0000255"
FT   COILED          482..584
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   681 AA;  76786 MW;  C03313756A4CF2F3 CRC64;
     MSSPKNEMYY SLLEWFKTLN LNAPHSDAES LADGVALAQA LNQFAPESFT DGWLSKIKPG
     AVGSNWRLRM SNLKKVAQSV YDYYSEVLNY SLGDFAKPDV QRIAEKCDLG ELERLLQLVL
     GCAVNCTNKQ SYITEIMSLE EELQANIMRA LQELESTRNA SSSPEAGGVV SSLSRSSLSG
     ILDGKALQEE RDAMAQKCFE TEKKMLLLID EKTNLQQELQ KLQQEVSRME HSSTAIGDDG
     VSLGPVQTGS VRYNDLRRQL DLLKEELLQS EGAREDLKLK AHQQETDLLH MQIRIDELMK
     SSAEVTTLKD EVDVLRESND KLKICEAQLD TYKKKLEDYN DLKKQVRILE ERSADYVQQN
     AQFEEDAKRY ANTKGQVELF KKEIQDLHAK LDNESSKNVK LEFDNKNLES KNLALQRAKD
     SLLKERDNLR EVVDELKCGQ LSSSSALAGN TVSTELQPLA TVDKLQRLEA ENKALREGQG
     GQTALAQLLD EANKRNEHLR EQLKTANERI LSLSHATQSD DPIFKENEFG KQIKQLMELN
     EQKTLQLEEA VTQSSTMQCK VTQLETNLAA REQEILAYDA KYRKCVEKAK EVIKSIDPRI
     ASALDASVLE KNAEVVEEEP KPKMTVMEEQ LMTSAFYRLG VNAQRDAIDS KLAILMGSGQ
     TFLARQRQSA PRKSLSAMKS K