ID   HOOK_DROGR              Reviewed;         683 AA.
AC   B4JAL5;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Protein hook;
GN   Name=hook {ECO:0000250|UniProtKB:Q24185};
GN   Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GH10889;
OS   Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Hawaiian Drosophila.
OX   NCBI_TaxID=7222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15287-2541.00;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC       of the endocytic pathway. Probably acts as a cytoskeletal linker
CC       protein required to tether endosome vesicles to the cytoskeleton.
CC       Involved in modulation of endocytosis at stages required for down-
CC       regulation of membrane proteins that control synapse size. Not involved
CC       in synaptic vesicle recycling. Required in R7 cells for boss
CC       endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC       adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q24185}. Endosome
CC       {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC       Note=Enriched at neuromuscular synapses, in both presynaptic and
CC       postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
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DR   EMBL; CH916368; EDW02801.1; -; Genomic_DNA.
DR   RefSeq; XP_001987934.1; XM_001987898.1.
DR   AlphaFoldDB; B4JAL5; -.
DR   SMR; B4JAL5; -.
DR   STRING; 7222.FBpp0144795; -.
DR   EnsemblMetazoa; FBtr0146303; FBpp0144795; FBgn0118370.
DR   GeneID; 6561923; -.
DR   KEGG; dgr:6561923; -.
DR   eggNOG; ENOG502QQM8; Eukaryota.
DR   HOGENOM; CLU_011214_1_0_1; -.
DR   InParanoid; B4JAL5; -.
DR   OMA; LEFNNHA; -.
DR   OrthoDB; 398210at2759; -.
DR   PhylomeDB; B4JAL5; -.
DR   Proteomes; UP000001070; Unassembled WGS sequence.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR   GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR036872; CH_dom_sf.
DR   InterPro; IPR008636; Hook_C.
DR   InterPro; IPR043936; HOOK_N.
DR   Pfam; PF05622; HOOK; 1.
DR   Pfam; PF19047; HOOK_N; 1.
DR   PROSITE; PS50021; CH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW   Endosome; Microtubule; Reference proteome; Synapse.
FT   CHAIN           1..683
FT                   /note="Protein hook"
FT                   /id="PRO_0000379064"
FT   DOMAIN          5..123
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   COILED          135..440
FT                   /evidence="ECO:0000255"
FT   COILED          484..594
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   683 AA;  77879 MW;  BD3ECB773E9A035B CRC64;
     MSTQNGMYYS LLEWFKTLNL NAPHANAEEL ADGVALAQAL NQFAPESFSD SWLSKIKSSA
     AGSNWRLRMS NLKKVVEGVY EYYSDVLNYT LQHDFVKPDV QAIAEKCDLT ELERLLQLVL
     GCAVNCAKKQ SYICEIMCLE EELQANIMRA LQELETSTRQ TTEGGVVSSL SRNSLSGMLD
     GNAKALQSQM TEERDAMAQK CFETEKKMLL LIDEKTNLQQ ELQKIQQEFA RLEHNTIGDD
     GVSLGPIQAG SVRYNELRRQ LELVKEELLQ SEGAREDLKI KAQQQETEML HMQQRIDELM
     KSTAVLITLK DEVDVLREST DKLKVCEAQL ETYKKKLEEY NDLKKHVKML EERSADYVQQ
     NAQFEEDAKR YANTKGQIEL FKKEIQDLHT KLDNESSKNV KLEFDNKNLD SKNLALQREK
     DNLLKERDNL REAFDELKCG QLTTNSGSLT GTTMSRELQP PAMMDKIQRL EAENKALREG
     QGGQTALAQL LDDANKRCEN LREQMKTANE RILSLSHASQ SDDPILKENE FSKQIKQLME
     LNEQKTLQIE EFATQNSTMQ CKITQLESSL NTREQEVMAY EVKYRKCVER AKEVIKNIDP
     RIASVLEANA LEKSVDVIEE ESKTKMSGME EQLMATAFYR LGVNAQRDAV DSKLALLMGS
     GQTFLARQRQ SAPRKSLTTM KSK