ID   HOOK_DROMO              Reviewed;         679 AA.
AC   B4KE73;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Protein hook;
GN   Name=hook {ECO:0000250|UniProtKB:Q24185};
GN   Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GI13042;
OS   Drosophila mojavensis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila.
OX   NCBI_TaxID=7230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 15081-1352.22;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC       of the endocytic pathway. Probably acts as a cytoskeletal linker
CC       protein required to tether endosome vesicles to the cytoskeleton.
CC       Involved in modulation of endocytosis at stages required for down-
CC       regulation of membrane proteins that control synapse size. Not involved
CC       in synaptic vesicle recycling. Required in R7 cells for boss
CC       endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC       adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q24185}. Endosome
CC       {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC       Note=Enriched at neuromuscular synapses, in both presynaptic and
CC       postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
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DR   EMBL; CH933807; EDW11818.1; -; Genomic_DNA.
DR   RefSeq; XP_002002376.1; XM_002002340.2.
DR   AlphaFoldDB; B4KE73; -.
DR   SMR; B4KE73; -.
DR   STRING; 7230.FBpp0162259; -.
DR   PRIDE; B4KE73; -.
DR   EnsemblMetazoa; FBtr0163767; FBpp0162259; FBgn0135799.
DR   GeneID; 6576385; -.
DR   KEGG; dmo:Dmoj_GI13042; -.
DR   eggNOG; ENOG502QQM8; Eukaryota.
DR   HOGENOM; CLU_011214_1_0_1; -.
DR   InParanoid; B4KE73; -.
DR   OMA; LEFNNHA; -.
DR   OrthoDB; 398210at2759; -.
DR   PhylomeDB; B4KE73; -.
DR   Proteomes; UP000009192; Unassembled WGS sequence.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR   GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR036872; CH_dom_sf.
DR   InterPro; IPR008636; Hook_C.
DR   InterPro; IPR043936; HOOK_N.
DR   Pfam; PF05622; HOOK; 1.
DR   Pfam; PF19047; HOOK_N; 1.
DR   PROSITE; PS50021; CH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW   Endosome; Microtubule; Reference proteome; Synapse.
FT   CHAIN           1..679
FT                   /note="Protein hook"
FT                   /id="PRO_0000379065"
FT   DOMAIN          5..123
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   COILED          140..627
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   679 AA;  77176 MW;  2FBC7811AFC1212E CRC64;
     MSAQNGMYYS LLEWFKTLNL NAPHANAEEL SDGVALAQAL NQFAPESFTN SWLSKIKSSA
     VGSNWRLRMS NLKKVVEGVY EYYSDVLNYT LQPDFAKPDV QAIAEKCDLT ELERLLQLVL
     GCAVNCAKKQ SYICEIMCLE EELQANIMRA LQDLESSTRQ SVEGGGVVSS MSRNSLSNML
     EGNTKAQEER DAMAQKCFET EKKMLLLIDE KNNLQQELQK IQQEFARLEL NTIGDDGVSL
     GPIQAGSVRY NELRRQLELV KEELLQSEGA REDLKIKAQQ QEADILHMQQ RIDELMKTTA
     ELTSLKDEVD VLRESTEKLK VCEAQLETYK KKLEEYNDLK KHVKMLEERS ADYVQQNAQF
     EEDAKRYANT KGQIELFKKE IQDLHTKLDN ESSKNVKLEF DNKNLESKAL ALQREKDSLL
     KERDNLREAF DELKCGQLST NSGSLTGNTV SRELQPAAMV DKIQRLEAEN KALREGQGGQ
     TALAQLLDDA NKRCEHLREQ IKAANERILS LTHASQSDDP ILKENELSKQ IKQLMELNEQ
     KTLQIEESST QNSAMQCKIT QLESTLSTRE QELMAYEVKY RKCVEKAKEV IKTIDPRIAS
     GLEANILEKS IDVIEEESKT KMSTMEEQLM TSAYYRLGVN AHRDAVESKL ALLMGSGQTF
     LARQRQSAPR KSLTTMKSK