ID   HOOK_DROSI              Reviewed;         679 AA.
AC   B4Q9E6;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Protein hook;
GN   Name=hook {ECO:0000250|UniProtKB:Q24185};
GN   Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GD21788;
OS   Drosophila simulans (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7240;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC       of the endocytic pathway. Probably acts as a cytoskeletal linker
CC       protein required to tether endosome vesicles to the cytoskeleton.
CC       Involved in modulation of endocytosis at stages required for down-
CC       regulation of membrane proteins that control synapse size. Not involved
CC       in synaptic vesicle recycling. Required in R7 cells for boss
CC       endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC       adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q24185}. Endosome
CC       {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC       Note=Enriched at neuromuscular synapses, in both presynaptic and
CC       postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
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DR   EMBL; CM000361; EDX05453.1; -; Genomic_DNA.
DR   RefSeq; XP_002079868.1; XM_002079832.2.
DR   AlphaFoldDB; B4Q9E6; -.
DR   SMR; B4Q9E6; -.
DR   STRING; 7240.B4Q9E6; -.
DR   EnsemblMetazoa; FBtr0221698; FBpp0220190; FBgn0193208.
DR   GeneID; 6732755; -.
DR   HOGENOM; CLU_011214_1_0_1; -.
DR   OMA; LEFNNHA; -.
DR   PhylomeDB; B4Q9E6; -.
DR   Proteomes; UP000000304; Chromosome 2l.
DR   Bgee; FBgn0193208; Expressed in embryo and 3 other tissues.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0031267; F:small GTPase binding; IEA:EnsemblMetazoa.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR   GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR036872; CH_dom_sf.
DR   InterPro; IPR008636; Hook_C.
DR   InterPro; IPR043936; HOOK_N.
DR   Pfam; PF05622; HOOK; 1.
DR   Pfam; PF19047; HOOK_N; 1.
DR   PROSITE; PS50021; CH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW   Endosome; Microtubule; Reference proteome; Synapse.
FT   CHAIN           1..679
FT                   /note="Protein hook"
FT                   /id="PRO_0000379069"
FT   DOMAIN          6..123
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   COILED          135..437
FT                   /evidence="ECO:0000255"
FT   COILED          480..574
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   679 AA;  76608 MW;  73314A82879B79A7 CRC64;
     MSAPKNEMYY SLLEWFKTLN LNAPHADAES LADGVALAQA LNQFAPESFT DAWLSKIKAS
     AVGSNWRLRM SNLKKVTQSV YDYYSDVLNY SLSDFSKPDL QRIAEKCDLG ELERLLQLVL
     GCAVNCAEKQ SYITEIMCLE EELQANIMRA LQELEATRQA STPEGGVASS LSRGSRTGLL
     DSKAVQEDRD ALAQKCFETE KKMLLLIDEK TNLQQELHKL QQEFARLEQH STVIGDDGVS
     LGPVQTGSVR YNELRRQLDL LKEELLQSEG AREDLKIKAQ QQDTDLLHMQ MRIEELMKSS
     AEVTTLKDEV DVLRESNDKL KICEAQLDTY KKKLEDYNDL KKQVKILEER SADYVQQNAQ
     FEEDAKRYAN TKGQVELFKK EIQDLHAKLD AESSKNVKLE FDNKNLEGKN LALQRAKDSL
     LKERDNLREA VDELKCGQLS SSTALTGTTV SRELQPSATV EKLQRLEAEN KALREGQGGQ
     TALAQLLDDA NKRCENLREQ LKTANERILS LSHASQSDDP ILKESEFGKQ IKQLMELNEQ
     KTLQLEEAVT QSTSLQCKVT QLETNLSARE QEILVYDAKY RKCVEKAKEV IKSIDPRIAS
     ALDASVLEKS ADLVEEEPKP KMSVMEEQLM TSAFYRLGVN AQRDAIDSKL AILMGSGQTF
     LARQRQSAPR KSLSAMKSK