ID   HOOK_DROWI              Reviewed;         692 AA.
AC   B4N1C2;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 2.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Protein hook;
GN   Name=hook {ECO:0000250|UniProtKB:Q24185};
GN   Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GK24183;
OS   Drosophila willistoni (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7260;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14030-0811.24;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC       of the endocytic pathway. Probably acts as a cytoskeletal linker
CC       protein required to tether endosome vesicles to the cytoskeleton.
CC       Involved in modulation of endocytosis at stages required for down-
CC       regulation of membrane proteins that control synapse size. Not involved
CC       in synaptic vesicle recycling. Required in R7 cells for boss
CC       endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC       adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q24185}. Endosome
CC       {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC       Note=Enriched at neuromuscular synapses, in both presynaptic and
CC       postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC   -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q24185}.
CC   -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDW78112.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CH963920; EDW78112.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_002067126.2; XM_002067090.2.
DR   AlphaFoldDB; B4N1C2; -.
DR   SMR; B4N1C2; -.
DR   STRING; 7260.FBpp0253326; -.
DR   EnsemblMetazoa; FBtr0254834; FBpp0253326; FBgn0226145.
DR   GeneID; 6643752; -.
DR   KEGG; dwi:6643752; -.
DR   eggNOG; ENOG502QQM8; Eukaryota.
DR   InParanoid; B4N1C2; -.
DR   OrthoDB; 398210at2759; -.
DR   Proteomes; UP000007798; Unassembled WGS sequence.
DR   GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR   GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR   GO; GO:0031267; F:small GTPase binding; IEA:EnsemblMetazoa.
DR   GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR   GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR   GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR   GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR   Gene3D; 1.10.418.10; -; 1.
DR   InterPro; IPR001715; CH-domain.
DR   InterPro; IPR036872; CH_dom_sf.
DR   InterPro; IPR008636; Hook_C.
DR   InterPro; IPR043936; HOOK_N.
DR   Pfam; PF05622; HOOK; 1.
DR   Pfam; PF19047; HOOK_N; 1.
DR   PROSITE; PS50021; CH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW   Endosome; Microtubule; Reference proteome; Synapse.
FT   CHAIN           1..692
FT                   /note="Protein hook"
FT                   /id="PRO_0000379070"
FT   DOMAIN          6..123
FT                   /note="Calponin-homology (CH)"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT   REGION          161..180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          135..576
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   692 AA;  77908 MW;  3CB7159E73E55A61 CRC64;
     MSEAKSEMYY SLLEWFKTLN LSAAHNNAEE LADGLALAQA LNQFAADFFT DSWLAKIKAS
     AVGSNWRLRM SNLKKVTQSL YDYYSEVMNY TLSDFVRPDL QLIAEKCDQV ELERLLQLVL
     GCAVNCAKKQ SYITEIMGLE EELQANIMRA LQELESTGLV AGERSPSSSA SGGAGSGGAV
     NSLSRNSLSV KALQEERDAM AQKCFETEKK MLLLLDEKTN LQQELQKIQQ EFARLEINTT
     AIGDDGVSLG PVQAGSVRFN EMRRQLDLLK EELLQSEGAR EDLKLKAVQQ ESDLVHMQQR
     IDELLKSSAE VTTLKDEVDV LRESNDKLKI CEAQLDTYKK KLEDYNDLKK QIKILEERSA
     DYVQQNAQFE DDAKRYANTK GQIELFKKEI QDLHSKLDIE SGKNVKLEFD NKNLESKNLA
     LQRAKESLVK ERDNLRETVD ELKCGHLTNN SGLTGCVEGT TMSRELQPTA MMDKIQRLEA
     ENKALREGQG GQTALAQLLD DANKRCEHLR DQLKSANERI LSLSHASQSD DPILKENEFA
     KQIKQLMELN EQKTLQLEEA VTQNASYQSK VIQLETSLSS REQEILACDA KYRKCVQKAK
     EIIKNIDPHI ANAIDASALE KNMVDVVDEE SKPKMSVMEE QLMTTAFYRL GVNAQRDAVD
     SKLAILMGSG QTFLARQRQS APRKSLSAMK SK