HOOK_DROYA
ID HOOK_DROYA Reviewed; 679 AA.
AC B4PAF2;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Protein hook;
GN Name=hook {ECO:0000250|UniProtKB:Q24185};
GN Synonyms=hk {ECO:0000250|UniProtKB:Q24185}; ORFNames=GE12681;
OS Drosophila yakuba (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tai18E2 / Tucson 14021-0261.01;
RX PubMed=17994087; DOI=10.1038/nature06341;
RG Drosophila 12 genomes consortium;
RT "Evolution of genes and genomes on the Drosophila phylogeny.";
RL Nature 450:203-218(2007).
CC -!- FUNCTION: Involved in endocytic trafficking by stabilizing organelles
CC of the endocytic pathway. Probably acts as a cytoskeletal linker
CC protein required to tether endosome vesicles to the cytoskeleton.
CC Involved in modulation of endocytosis at stages required for down-
CC regulation of membrane proteins that control synapse size. Not involved
CC in synaptic vesicle recycling. Required in R7 cells for boss
CC endocytosis into multivesicular bodies (MVBs). Has a role in regulating
CC adult longevity. {ECO:0000250|UniProtKB:Q24185}.
CC -!- SUBUNIT: Homodimer. Interacts with microtubules via its N-terminus.
CC {ECO:0000250|UniProtKB:Q24185}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q24185}. Endosome
CC {ECO:0000250|UniProtKB:Q24185}. Synapse {ECO:0000250|UniProtKB:Q24185}.
CC Note=Enriched at neuromuscular synapses, in both presynaptic and
CC postsynaptic regions. {ECO:0000250|UniProtKB:Q24185}.
CC -!- DOMAIN: The coiled coil domain mediates homodimerization.
CC {ECO:0000250|UniProtKB:Q24185}.
CC -!- SIMILARITY: Belongs to the hook family. {ECO:0000305}.
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DR EMBL; CM000158; EDW90360.1; -; Genomic_DNA.
DR RefSeq; XP_002090648.1; XM_002090612.2.
DR AlphaFoldDB; B4PAF2; -.
DR SMR; B4PAF2; -.
DR STRING; 7245.FBpp0257691; -.
DR EnsemblMetazoa; FBtr0259199; FBpp0257691; FBgn0230407.
DR GeneID; 6529655; -.
DR KEGG; dya:Dyak_GE12681; -.
DR eggNOG; ENOG502QQM8; Eukaryota.
DR HOGENOM; CLU_011214_1_0_1; -.
DR OMA; LEFNNHA; -.
DR OrthoDB; 398210at2759; -.
DR PhylomeDB; B4PAF2; -.
DR Proteomes; UP000002282; Chromosome 2R.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0045202; C:synapse; ISS:UniProtKB.
DR GO; GO:0008017; F:microtubule binding; ISS:UniProtKB.
DR GO; GO:0031267; F:small GTPase binding; IEA:EnsemblMetazoa.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IEA:InterPro.
DR GO; GO:0030705; P:cytoskeleton-dependent intracellular transport; ISS:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR GO; GO:0006897; P:endocytosis; ISS:UniProtKB.
DR Gene3D; 1.10.418.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR008636; Hook_C.
DR InterPro; IPR043936; HOOK_N.
DR Pfam; PF05622; HOOK; 1.
DR Pfam; PF19047; HOOK_N; 1.
DR PROSITE; PS50021; CH; 1.
PE 3: Inferred from homology;
KW Coiled coil; Cytoplasm; Cytoskeleton; Developmental protein; Endocytosis;
KW Endosome; Microtubule; Synapse.
FT CHAIN 1..679
FT /note="Protein hook"
FT /id="PRO_0000379071"
FT DOMAIN 6..123
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT COILED 135..437
FT /evidence="ECO:0000255"
FT COILED 480..574
FT /evidence="ECO:0000255"
SQ SEQUENCE 679 AA; 76552 MW; 8D2B3AFB64A84FD5 CRC64;
MSAPKNEMYY SLLEWFKTLN LNAPHADAES LADGVALAQA LNQFAPESFT DAWLSKIKAS
AVGSNWRLRM SNLKKVTQSV YDYYSDVLNY SLSDFSKPDL QSIAEKCDLG ELERLLQLVL
GCAVNCAEKQ SYITEIMCLE EELQANIMRA LQELEATRQA SSAEGGVVSS LSRSPRTGIL
DSKAVQEDRD ALAQKCFETE KKMLLLIDEK TNLQQELHKL QQEFARLEQH STVIGDDGVS
LGPVQSGSVR YNELRRQLDL LKEELLQSEG AREDLKLKAQ QQDTDLLHMQ MRIEELMKSS
AEVTTLKDEV DVLRESNDKL KICEAQLDTY KKKLEDYNDL KKQVKILEER SADYVQQNAQ
FEEDAKRYAN TKGQVELFKK EIQDLHAKLD AESSKNVKLE FDNKNLDGKN LALQRAKDSL
LKERDNLREA VDELKCGQLS SNTALTGTTV SRELQPSATV EKLQRLEAEN KALREGQGGQ
TALAQLLDDA NKRCENLREQ LKTANERILS LSHASQSDDP ILKESEFGKQ IKQLMELNEQ
KTLQLEEAVT QSTSLQCKVT QLETNLSARE QEILVYDAKY RKCVEKAKEV IKSIDPRIAS
ALDASVLEKS ADLVEDEPKP KMSVMEEQLM TSAFYRLGVN AQRDAIDSKL AILMGSGQTF
LARQRQSAPR KSLSAMKSK