HOP2_YEAST
ID HOP2_YEAST Reviewed; 214 AA.
AC P53187; D6VUA5; O74245;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2015, sequence version 4.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Homologous-pairing protein 2;
GN Name=HOP2; OrderedLocusNames=YGL033W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-203, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=YAB36;
RX PubMed=9708739; DOI=10.1016/s0092-8674(00)81480-4;
RA Leu J.-Y., Chua P.R., Roeder G.S.;
RT "The meiosis-specific Hop2 protein of S. cerevisiae ensures synapsis
RT between homologous chromosomes.";
RL Cell 94:375-386(1998).
RN [4]
RP PROTEIN SEQUENCE OF 2-6, FUNCTION, INTERACTION WITH MND1, AND DNA-BINDING.
RX PubMed=15249670; DOI=10.1073/pnas.0404195101;
RA Chen Y.-K., Leng C.-H., Olivares H., Lee M.-H., Chang Y.-C., Kung W.-M.,
RA Ti S.-C., Lo Y.-H., Wang A.H.-J., Chang C.-S., Bishop D.K., Hsueh Y.-P.,
RA Wang T.-F.;
RT "Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote
RT meiotic homolog juxtaposition and strand assimilation.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:10572-10577(2004).
RN [5]
RP FUNCTION, AND INTERACTION WITH MND1.
RX PubMed=11940665; DOI=10.1128/mcb.22.9.3078-3088.2002;
RA Tsubouchi H., Roeder G.S.;
RT "The Mnd1 protein forms a complex with Hop2 to promote homologous
RT chromosome pairing and meiotic double-strand break repair.";
RL Mol. Cell. Biol. 22:3078-3088(2002).
RN [6]
RP REVISION OF GENE MODEL.
RX PubMed=24798326; DOI=10.1074/jbc.m114.558601;
RA Chan Y.L., Brown M.S., Qin D., Handa N., Bishop D.K.;
RT "The third exon of the budding yeast meiotic recombination gene HOP2 is
RT required for calcium-dependent and recombinase Dmc1-specific stimulation of
RT homologous strand assimilation.";
RL J. Biol. Chem. 289:18076-18086(2014).
CC -!- FUNCTION: Required for proper homologous chromosome pairing and
CC efficient cross-over and intragenic recombination during meiosis.
CC Stimulates DMC1-dependent homologous strand assimilation required for
CC the resolution of meiotic double-strand breaks.
CC {ECO:0000269|PubMed:11940665, ECO:0000269|PubMed:15249670,
CC ECO:0000269|PubMed:24798326, ECO:0000269|PubMed:9708739}.
CC -!- SUBUNIT: Heterodimer with MND1. MND1-HOP2 binds preferentially to
CC dsDNA.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9708739}.
CC -!- SIMILARITY: Belongs to the HOP2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC31823.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAC31823.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA96734.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z72555; CAA96734.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF078740; AAC31823.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006941; DAA08066.2; -; Genomic_DNA.
DR PIR; S64035; S64035.
DR RefSeq; NP_011482.3; NM_001180898.2.
DR AlphaFoldDB; P53187; -.
DR SMR; P53187; -.
DR BioGRID; 33213; 52.
DR ComplexPortal; CPX-1644; HOP2-MND1 complex.
DR IntAct; P53187; 1.
DR STRING; 4932.YGL033W; -.
DR CarbonylDB; P53187; -.
DR PaxDb; P53187; -.
DR PRIDE; P53187; -.
DR EnsemblFungi; YGL033W_mRNA; YGL033W; YGL033W.
DR GeneID; 852850; -.
DR KEGG; sce:YGL033W; -.
DR SGD; S000003001; HOP2.
DR VEuPathDB; FungiDB:YGL033W; -.
DR eggNOG; KOG4603; Eukaryota.
DR HOGENOM; CLU_063266_2_0_1; -.
DR InParanoid; P53187; -.
DR BioCyc; YEAST:G3O-30549-MON; -.
DR PRO; PR:P53187; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53187; protein.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:SGD.
DR GO; GO:0120231; C:DNA recombinase auxiliary factor complex; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:SGD.
DR GO; GO:0120230; F:recombinase activator activity; IBA:GO_Central.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IDA:ComplexPortal.
DR GO; GO:0000709; P:meiotic joint molecule formation; IBA:GO_Central.
DR GO; GO:0010774; P:meiotic strand invasion involved in reciprocal meiotic recombination; IBA:GO_Central.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IDA:ComplexPortal.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR040461; Hop2.
DR InterPro; IPR010776; Hop2_WH_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR15938; PTHR15938; 1.
DR Pfam; PF07106; TBPIP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Direct protein sequencing; DNA recombination; DNA-binding;
KW Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..214
FT /note="Homologous-pairing protein 2"
FT /id="PRO_0000084027"
FT COILED 97..160
FT /evidence="ECO:0000255"
FT CONFLICT 71
FT /note="G -> A (in Ref. 3; AAC31823)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 214 AA; 24479 MW; F60B3809A48F50A6 CRC64;
MAPKKKSNDR AIQAKGSEAE QLIEDYLVSQ YKPFSVNDIV QNLHNKVTKT TATKALENLV
NEKRIVSKTF GKIIIYSCNE QDTALPSNID PSQFDFETVL QLRNDLIELE RDKSTAKDAL
DSVTKEPENE DLLTIIENEE NELKKIESKL QSLQDDWDPA NDEIVKRIMS EDTLLQKEIT
KRSKICKNLI ATIKDSVCPK NMNEFLEEIG FEDI
