HOPM1_PSESM
ID HOPM1_PSESM Reviewed; 712 AA.
AC Q887D0; Q9JP36;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Effector protein HopM1;
DE AltName: Full=Hrp outer protein M1;
DE AltName: Full=Type III effector HopPtoM;
GN Name=hopM1; Synonyms=holPtoX, hopPtoM; OrderedLocusNames=PSPTO_1375;
GN ORFNames=CEL ORF3;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=10781092; DOI=10.1073/pnas.97.9.4856;
RA Alfano J.R., Charkowski A.O., Deng W.-L., Badel J.L., Petnicki-Ocwieja T.,
RA van Dijk K., Collmer A.;
RT "The Pseudomonas syringae Hrp pathogenicity island has a tripartite mosaic
RT structure composed of a cluster of type III secretion genes bounded by
RT exchangeable effector and conserved effector loci that contribute to
RT parasitic fitness and pathogenicity in plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:4856-4861(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=11967070; DOI=10.1046/j.1365-2958.2002.02877.x;
RA Boch J., Joardar V., Gao L., Robertson T.L., Lim M., Kunkel B.N.;
RT "Identification of Pseudomonas syringae pv. tomato genes induced during
RT infection of Arabidopsis thaliana.";
RL Mol. Microbiol. 44:73-88(2002).
RN [4]
RP FUNCTION, INDUCTION, INTERACTION WITH SHCM, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12940984; DOI=10.1046/j.1365-2958.2003.03647.x;
RA Badel J.L., Nomura K., Bandyopadhyay S., Shimizu R., Collmer A., He S.Y.;
RT "Pseudomonas syringae pv. tomato DC3000 HopPtoM (CEL ORF3) is important for
RT lesion formation but not growth in tomato and is secreted and translocated
RT by the Hrp type III secretion system in a chaperone-dependent manner.";
RL Mol. Microbiol. 49:1239-1251(2003).
RN [5]
RP FUNCTION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=15210989; DOI=10.1073/pnas.0401601101;
RA DebRoy S., Thilmony R., Kwack Y.-B., Nomura K., He S.Y.;
RT "A family of conserved bacterial effectors inhibits salicylic acid-mediated
RT basal immunity and promotes disease necrosis in plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9927-9932(2004).
RN [6]
RP FUNCTION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=16212612; DOI=10.1111/j.1365-313x.2005.02529.x;
RA Oh H.-S., Collmer A.;
RT "Basal resistance against bacteria in Nicotiana benthamiana leaves is
RT accompanied by reduced vascular staining and suppressed by multiple
RT Pseudomonas syringae type III secretion system effector proteins.";
RL Plant J. 44:348-359(2005).
RN [7]
RP FUNCTION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=16529372; DOI=10.1094/mpmi-19-0099;
RA Badel J.L., Shimizu R., Oh H.-S., Collmer A.;
RT "A Pseudomonas syringae pv. tomato avrE1/hopM1 mutant is severely reduced
RT in growth and lesion formation in tomato.";
RL Mol. Plant Microbe Interact. 19:99-111(2006).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH BIG5/ATMIN7.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=16840699; DOI=10.1126/science.1129523;
RA Nomura K., DebRoy S., Lee Y.H., Pumplin N., Jones J., He S.Y.;
RT "A bacterial virulence protein suppresses host innate immunity to cause
RT plant disease.";
RL Science 313:220-223(2006).
CC -!- FUNCTION: Involved in the suppression of basal resistance and promotion
CC of disease symptoms in plants. Mediates the ubiquitination and
CC degradation, via the host proteasome, of a low-abundance immunity-
CC associated protein in Arabidopsis thaliana. May be involved in the
CC inhibition of a host vesicle trafficking pathway.
CC {ECO:0000269|PubMed:12940984, ECO:0000269|PubMed:15210989,
CC ECO:0000269|PubMed:16212612, ECO:0000269|PubMed:16529372,
CC ECO:0000269|PubMed:16840699}.
CC -!- SUBUNIT: Interacts with the chaperone ShcM. Interacts with host plant
CC BIG5/ATMIN7. {ECO:0000269|PubMed:12940984,
CC ECO:0000269|PubMed:16840699}.
CC -!- INTERACTION:
CC Q887D0; F4IXW2: BIG5; Xeno; NbExp=3; IntAct=EBI-6394949, EBI-6395043;
CC -!- SUBCELLULAR LOCATION: Secreted. Host membrane. Note=Secreted via the
CC type III secretion system (TTSS). Localized to the plant endomembrane.
CC -!- INDUCTION: Transcriptionally induced by HrpL.
CC {ECO:0000269|PubMed:11967070, ECO:0000269|PubMed:12940984}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF71501.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF232004; AAF71501.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE016853; AAO54897.1; -; Genomic_DNA.
DR RefSeq; NP_791202.1; NC_004578.1.
DR RefSeq; WP_005763919.1; NC_004578.1.
DR AlphaFoldDB; Q887D0; -.
DR IntAct; Q887D0; 8.
DR STRING; 223283.PSPTO_1375; -.
DR EnsemblBacteria; AAO54897; AAO54897; PSPTO_1375.
DR GeneID; 1183011; -.
DR KEGG; pst:PSPTO_1375; -.
DR PATRIC; fig|223283.9.peg.1397; -.
DR eggNOG; ENOG5031JIP; Bacteria.
DR HOGENOM; CLU_023400_0_0_6; -.
DR OMA; EHFQLMH; -.
DR OrthoDB; 85709at2; -.
DR PHI-base; PHI:4008; -.
DR PHI-base; PHI:6378; -.
DR PHI-base; PHI:982; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Host membrane; Membrane; Reference proteome; Secreted; Virulence.
FT CHAIN 1..712
FT /note="Effector protein HopM1"
FT /id="PRO_0000260212"
FT REGION 22..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 13
FT /note="E -> K (in Ref. 1; AAF71501)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 712 AA; 75214 MW; 56B67FFEFE3231D2 CRC64;
MISSRIGGAG GVELSRVNQQ HDTVPAQTAH PNAVTAGMNP PLTPDQSGSH ATESSSAGAA
RLNVAARHTQ LLQAFKAEHG TAPVSGAPMI SSRAALLIGS LLQAEPLPFE VMAEKLSPER
YQLKQFQGSD LQQRLEKFAQ PGQIPDKAEV GQLIKGFAQS VADQLEHFQL MHDASPATVG
QHAKADKATL AVSQTALGEY AGRASKAIGE GLSNSIASLD EHISALDLTL QDAEQGNKES
LHADRQALVD AKTTLVGLHA DFVKSPEAKR LASVAAHTQL DNVVSDLVTA RNTVGGWKGA
GPIVAAAVPQ FLSSMTHLGY VRLSTSDKLR DTIPETSSDA NMLKASIIGM VAGIAHETVN
SVVKPMFQAA LQKTGLNERL NMVPMKAVDT NTVIPDPFEL KSEHGELVKK TPEEVAQDKA
FVKSERALLN QKKVQGSSTH PVGELMAYSA FGGSQAVRQM LNDVHQINGQ TLSARALASG
FGGAVSASSQ TLLQLKSNYV DPQGRKIPVF TPDRAESDLK KDLLKGMDLR EPSVRTTFYS
KALSGIQSSA LTSALPPVTA QAEGASGTLS AGAILRNMAL AATGSVSYLS TLYTNQSVTA
EAKALKAAGM GGATPMLDRT ETALNNIRHP NRESLPHTFQ KSTLSGIPRV AENAYHMGRG
ALQLPTQMAV DTVRVVDEGV LNAVASAREA LKQPTKDDDA LRALEEGLLD PR
