ID   HOT13_KLULA             Reviewed;         114 AA.
AC   Q6CIK2;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Helper of Tim protein 13;
GN   Name=HOT13; OrderedLocusNames=KLLA0F25982g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for the assembly or recycling of the small Tim
CC       proteins in the mitochondrial intermembrane, thereby participating in
CC       the import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the small Tim proteins. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion intermembrane space {ECO:0000250}.
CC       Mitochondrion membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}.
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DR   EMBL; CR382126; CAG98945.1; -; Genomic_DNA.
DR   RefSeq; XP_456237.1; XM_456237.1.
DR   AlphaFoldDB; Q6CIK2; -.
DR   STRING; 28985.XP_456237.1; -.
DR   EnsemblFungi; CAG98945; CAG98945; KLLA0_F25982g.
DR   GeneID; 2895135; -.
DR   KEGG; kla:KLLA0_F25982g; -.
DR   eggNOG; KOG1940; Eukaryota.
DR   HOGENOM; CLU_143932_0_0_1; -.
DR   InParanoid; Q6CIK2; -.
DR   OMA; ETRCAHY; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008270; F:zinc ion binding; IEA:EnsemblFungi.
DR   GO; GO:0045041; P:protein import into mitochondrial intermembrane space; IEA:EnsemblFungi.
DR   InterPro; IPR016694; UCP017292.
DR   InterPro; IPR008913; Znf_CHY.
DR   InterPro; IPR037274; Znf_CHY_sf.
DR   Pfam; PF05495; zf-CHY; 1.
DR   PIRSF; PIRSF017292; UCP017292_Znf_CHY; 1.
DR   SUPFAM; SSF161219; SSF161219; 1.
DR   PROSITE; PS51266; ZF_CHY; 1.
PE   3: Inferred from homology;
KW   Membrane; Metal-binding; Mitochondrion; Protein transport;
KW   Reference proteome; Translocation; Transport; Zinc; Zinc-finger.
FT   CHAIN           1..114
FT                   /note="Helper of Tim protein 13"
FT                   /id="PRO_0000228009"
FT   ZN_FING         10..90
FT                   /note="CHY-type; degenerate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         17
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         19
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         40
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         43
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         67
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         70
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         85
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT   BINDING         88
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
SQ   SEQUENCE   114 AA;  13338 MW;  45968FB679312FF1 CRC64;
     MEKAQIYGKL VDKESRCEHW HGPLDVIALR FKCCNGYYAC YECHQELVPH VTERYNINDE
     TVPLVICGVC KLEMSFAAYS DSLQCPNCRS QFNPGCKLHY DMYFMKDDEP ENLR