HOT13_YEAST
ID HOT13_YEAST Reviewed; 116 AA.
AC P36078; D6VXK4; Q6Q598;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Helper of Tim protein 13;
GN Name=HOT13; OrderedLocusNames=YKL084W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15294910; DOI=10.1074/jbc.m404878200;
RA Curran S.P., Leuenberger D., Leverich E.P., Hwang D.K., Beverly K.N.,
RA Koehler C.M.;
RT "The role of Hot13p and redox chemistry in the mitochondrial TIM22 import
RT pathway.";
RL J. Biol. Chem. 279:43744-43751(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for the assembly or recycling of the small Tim
CC proteins in the mitochondrial intermembrane, thereby participating in
CC the import and insertion of multi-pass transmembrane proteins into the
CC mitochondrial inner membrane. Probably acts by facilitating the
CC formation of disulfide bonds in small Tim proteins.
CC {ECO:0000269|PubMed:15294910}.
CC -!- SUBUNIT: Interacts with the small Tim proteins TIM8, TIM9, TIM10,
CC TIM12, and TIM13.
CC -!- SUBCELLULAR LOCATION: Mitochondrion intermembrane space
CC {ECO:0000269|PubMed:15294910}. Mitochondrion membrane
CC {ECO:0000269|PubMed:15294910}; Peripheral membrane protein
CC {ECO:0000269|PubMed:15294910}.
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DR EMBL; Z28084; CAA81922.1; -; Genomic_DNA.
DR EMBL; AY558265; AAS56591.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09074.1; -; Genomic_DNA.
DR PIR; S37909; S37909.
DR RefSeq; NP_012839.1; NM_001179650.1.
DR AlphaFoldDB; P36078; -.
DR BioGRID; 34049; 79.
DR IntAct; P36078; 2.
DR MINT; P36078; -.
DR STRING; 4932.YKL084W; -.
DR MaxQB; P36078; -.
DR PaxDb; P36078; -.
DR PRIDE; P36078; -.
DR EnsemblFungi; YKL084W_mRNA; YKL084W; YKL084W.
DR GeneID; 853778; -.
DR KEGG; sce:YKL084W; -.
DR SGD; S000001567; HOT13.
DR VEuPathDB; FungiDB:YKL084W; -.
DR eggNOG; KOG1940; Eukaryota.
DR HOGENOM; CLU_143932_0_0_1; -.
DR InParanoid; P36078; -.
DR OMA; ETRCAHY; -.
DR BioCyc; YEAST:G3O-31878-MON; -.
DR PRO; PR:P36078; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36078; protein.
DR GO; GO:0005758; C:mitochondrial intermembrane space; IDA:SGD.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008270; F:zinc ion binding; IDA:SGD.
DR GO; GO:0045041; P:protein import into mitochondrial intermembrane space; IMP:SGD.
DR InterPro; IPR016694; UCP017292.
DR InterPro; IPR008913; Znf_CHY.
DR InterPro; IPR037274; Znf_CHY_sf.
DR Pfam; PF05495; zf-CHY; 1.
DR PIRSF; PIRSF017292; UCP017292_Znf_CHY; 1.
DR SUPFAM; SSF161219; SSF161219; 1.
DR PROSITE; PS51266; ZF_CHY; 1.
PE 1: Evidence at protein level;
KW Membrane; Metal-binding; Mitochondrion; Protein transport;
KW Reference proteome; Translocation; Transport; Zinc; Zinc-finger.
FT CHAIN 1..116
FT /note="Helper of Tim protein 13"
FT /id="PRO_0000203165"
FT ZN_FING 10..94
FT /note="CHY-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 17
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 19
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 40
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 43
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 68
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 71
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT BINDING 92
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00601"
FT CONFLICT 71
FT /note="C -> R (in Ref. 3; AAS56591)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 116 AA; 13641 MW; 57464E5FD9A591F8 CRC64;
MIETAIYGKT VDDQSRCVHW HLPKDVIAIR FKCCDKYYAC FECHQELSSH PLEKYDLLDD
ANKHLIICGV CRHEMTFAEY YDYNSNLICP NCRSPFNPGC KLHYHLYFQN PPPAMC
