ID   3SPT_DENAN              Reviewed;          59 AA.
AC   P81946;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Thrombostatin;
DE   AltName: Full=Glycoprotein IIb-IIIa antagonist;
DE   AltName: Full=Platelet aggregation inhibitor;
OS   Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX   NCBI_TaxID=8618;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RA   Valant P.A., Potter L.T.;
RT   "Thrombostatin, a potent inhibitor of platelet aggregation from the venom
RT   of the green mamba.";
RL   Submitted (JAN-1999) to UniProtKB.
CC   -!- FUNCTION: Inhibits ADP-induced platelet aggregation and inhibits the
CC       binding of purified platelet fibrinogen receptor alpha-IIb/beta-3
CC       (ITGA2B/ITGB3) to immobilized fibrinogen.
CC       {ECO:0000250|UniProtKB:P28375}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Antiplatelet toxin sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P81946; -.
DR   SMR; P81946; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Cell adhesion impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Hemostasis impairing toxin; Platelet aggregation inhibiting toxin;
KW   Secreted; Toxin.
FT   CHAIN           1..59
FT                   /note="Thrombostatin"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000093657"
FT   MOTIF           43..45
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   DISULFID        3..22
FT                   /evidence="ECO:0000250|UniProtKB:P28375"
FT   DISULFID        17..37
FT                   /evidence="ECO:0000250|UniProtKB:P28375"
FT   DISULFID        39..51
FT                   /evidence="ECO:0000250|UniProtKB:P28375"
FT   DISULFID        52..57
FT                   /evidence="ECO:0000250|UniProtKB:P28375"
SQ   SEQUENCE   59 AA;  6670 MW;  131E8B92217D9226 CRC64;
     LICYNQLGTK PPTTETCGDD SCYKMIWTYD GVIRRGCGCF TPRGDMPRPR CCKSDKCNL