ID   HOXH_RHOOP              Reviewed;          38 AA.
AC   P22661;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=NAD-reducing hydrogenase HoxS subunit beta;
DE            EC=1.12.1.2;
DE   Flags: Fragment;
GN   Name=hoxH;
OS   Rhodococcus opacus (Nocardia opaca).
OG   Plasmid.
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=37919;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=1B;
RX   PubMed=2496982; DOI=10.1111/j.1432-1033.1989.tb14708.x;
RA   Zaborosch C., Schneider K., Schlegel H.G., Kratzin H.;
RT   "Comparison of the NH2-terminal amino acid sequences of the four non-
RT   identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b
RT   and Alcaligenes eutrophus H16.";
RL   Eur. J. Biochem. 181:175-180(1989).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2 + NAD(+) = H(+) + NADH; Xref=Rhea:RHEA:24636,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.12.1.2;
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC   -!- SUBUNIT: Tetramer of an alpha and a gamma subunits (flavin-containing
CC       dimer), and a delta and a nickel-containing beta subunits (hydrogenase
CC       dimer).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
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DR   AlphaFoldDB; P22661; -.
DR   SMR; P22661; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0047985; F:hydrogen dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   SUPFAM; SSF56762; SSF56762; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Metal-binding; NAD; Nickel;
KW   Oxidoreductase; Plasmid.
FT   CHAIN           1..>38
FT                   /note="NAD-reducing hydrogenase HoxS subunit beta"
FT                   /id="PRO_0000199720"
FT   NON_TER         38
SQ   SEQUENCE   38 AA;  4219 MW;  227487B5299C29C2 CRC64;
     STKLVIDPVT RIEGHGKVTV HLDDNNNVVD AHLHVVEF