HP302_ARATH
ID HP302_ARATH Reviewed; 259 AA.
AC Q9FLT9; Q8L9N9;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Chloroplastic import inner membrane translocase subunit HP30-2 {ECO:0000305};
DE AltName: Full=Hypothetical inner plastid envelope protein of 30 kDa 2 {ECO:0000303|PubMed:24248378};
DE Short=AtHP30-2 {ECO:0000303|PubMed:24248378};
DE Short=Hypothetical protein 30-2 {ECO:0000303|PubMed:24248378};
GN Name=HP30-2 {ECO:0000303|PubMed:24248378};
GN OrderedLocusNames=At5g24650 {ECO:0000312|Araport:AT5G24650};
GN ORFNames=K18P6.19 {ECO:0000312|EMBL:AED93344.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND GENE FAMILY.
RX PubMed=17098851; DOI=10.1104/pp.106.090688;
RA Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M.,
RA Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.;
RT "Characterization of the preprotein and amino acid transporter gene family
RT in Arabidopsis.";
RL Plant Physiol. 143:199-212(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION.
RX PubMed=15901827; DOI=10.1074/mcp.m500018-mcp200;
RA von Zychlinski A., Kleffmann T., Krishnamurthy N., Sjoelander K.,
RA Baginsky S., Gruissem W.;
RT "Proteome analysis of the rice etioplast: metabolic and regulatory networks
RT and novel protein functions.";
RL Mol. Cell. Proteomics 4:1072-1084(2005).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, SUBUNIT, AND
RP INTERACTION WITH CEQORH.
RC STRAIN=cv. Columbia;
RX PubMed=24248378; DOI=10.1073/pnas.1319648110;
RA Rossig C., Reinbothe C., Gray J., Valdes O., von Wettstein D.,
RA Reinbothe S.;
RT "Three proteins mediate import of transit sequence-less precursors into the
RT inner envelope of chloroplasts in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:19962-19967(2013).
CC -!- FUNCTION: Together with HP30-1 and HP20, triggers the import and
CC insertion of transit sequence-less multi-pass transmembrane proteins
CC (e.g. CEQORH) into the chloroplastic inner membrane.
CC {ECO:0000269|PubMed:24248378}.
CC -!- SUBUNIT: Probable component of a protein-conducting channel made of
CC HP30-1, HP30-2 and HP20 that mediates the import of transit sequence-
CC less proteins into the chloroplastic inner membrane. Interacts with
CC CEQORH. {ECO:0000269|PubMed:24248378}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:17098851}; Multi-pass membrane protein
CC {ECO:0000255}. Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:17098851, ECO:0000269|PubMed:24248378}; Multi-pass
CC membrane protein {ECO:0000255}. Note=Seems to be present both in
CC chloroplast and mitochondrion. {ECO:0000269|PubMed:17098851}.
CC -!- DISRUPTION PHENOTYPE: Plants lacking both HP30-1 and HP30-2 are yellow
CC to pale-green and impaired import of CEQORH in chloroplast inner
CC membranes. {ECO:0000269|PubMed:24248378}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM65866.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ405267; ABD64056.1; -; mRNA.
DR EMBL; AB010068; BAB11217.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93344.1; -; Genomic_DNA.
DR EMBL; AY070735; AAL50076.1; -; mRNA.
DR EMBL; AY142048; AAM98312.1; -; mRNA.
DR EMBL; AY088327; AAM65866.1; ALT_INIT; mRNA.
DR RefSeq; NP_197853.1; NM_122373.3.
DR AlphaFoldDB; Q9FLT9; -.
DR SMR; Q9FLT9; -.
DR STRING; 3702.AT5G24650.1; -.
DR PaxDb; Q9FLT9; -.
DR PRIDE; Q9FLT9; -.
DR ProteomicsDB; 230265; -.
DR EnsemblPlants; AT5G24650.1; AT5G24650.1; AT5G24650.
DR GeneID; 832536; -.
DR Gramene; AT5G24650.1; AT5G24650.1; AT5G24650.
DR KEGG; ath:AT5G24650; -.
DR Araport; AT5G24650; -.
DR TAIR; locus:2153959; AT5G24650.
DR eggNOG; ENOG502QPIB; Eukaryota.
DR HOGENOM; CLU_072970_0_0_1; -.
DR InParanoid; Q9FLT9; -.
DR OMA; TWLAKQS; -.
DR OrthoDB; 1451194at2759; -.
DR PhylomeDB; Q9FLT9; -.
DR PRO; PR:Q9FLT9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLT9; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:InterPro.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IPI:TAIR.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0007005; P:mitochondrion organization; IGI:TAIR.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:InterPro.
DR GO; GO:0045036; P:protein targeting to chloroplast; IMP:UniProtKB.
DR GO; GO:0016031; P:tRNA import into mitochondrion; IGI:TAIR.
DR Gene3D; 1.10.150.50; -; 1.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
DR Pfam; PF00536; SAM_1; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Mitochondrion; Plastid; Plastid inner membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..259
FT /note="Chloroplastic import inner membrane translocase
FT subunit HP30-2"
FT /id="PRO_0000443725"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 259 AA; 27772 MW; 2E2C91BFE8CDF3C9 CRC64;
MGKDGEGDKK RETMAVMSLM KDQQNPIQQF QVKFKEIETG FKSWLSKQKL PVEAAVVTAM
GGVQGAFIGG LMGTLSPEMP QAGIDPQAMA SLKQTQALVG GPLVQARNFA AITGVNAGIA
CVMKRIRGKE DLESAVVAAF GSGVAYSLVS AGLQGQPMNA ITTAAGFAVF QGVFFKLGER
FSKPSVEDPY YTRGRSMLLK LGLEKYEKNF KKGLLADPTL PLLTDSALRD VSIPPGPRLL
ILDHIQRDPE LKGKRGSRG
