ID   HPAB_KLEOX              Reviewed;         520 AA.
AC   Q48440;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=4-hydroxyphenylacetate 3-monooxygenase oxygenase component;
DE            EC=1.14.14.9;
DE   AltName: Full=4-HPA 3-hydroxylase;
DE   AltName: Full=4-HPA 3-monooxygenase large component;
GN   Name=hpaB; Synonyms=hpaA;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M5a1;
RX   PubMed=9133323; DOI=10.1007/s002030050429;
RA   Gibello A., Suarez M., Allende J.L., Martin M.;
RT   "Molecular cloning and analysis of the genes encoding the 4-
RT   hydroxyphenylacetate hydroxylase from Klebsiella pneumoniae.";
RL   Arch. Microbiol. 167:160-166(1997).
CC   -!- FUNCTION: Utilizes FADH(2) supplied by HpaC or by another flavin
CC       reductase, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid,
CC       leading to the production of 3,4-DHPA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-hydroxyphenylacetate + FADH2 + O2 = 3,4-
CC         dihydroxyphenylacetate + FAD + H(+) + H2O; Xref=Rhea:RHEA:30595,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17612, ChEBI:CHEBI:48999, ChEBI:CHEBI:57692,
CC         ChEBI:CHEBI:58307; EC=1.14.14.9;
CC   -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC       degradation; pyruvate and succinate semialdehyde from 4-
CC       hydroxyphenylacetate: step 1/7.
CC   -!- SUBUNIT: 4-HPA 3-monooxygenase consists of a reductase component HpaC
CC       and an oxygenase component HpaB. {ECO:0000250}.
CC   -!- INDUCTION: By 3- or 4-hydroxyphenylacetic acid.
CC   -!- SIMILARITY: Belongs to the FADH(2)-utilizing monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; L41068; AAC37120.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48440; -.
DR   SMR; Q48440; -.
DR   STRING; 571.MC52_11760; -.
DR   eggNOG; COG2368; Bacteria.
DR   UniPathway; UPA00208; UER00416.
DR   GO; GO:0052881; F:4-hydroxyphenylacetate 3-monooxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0016627; F:oxidoreductase activity, acting on the CH-CH group of donors; IEA:InterPro.
DR   GO; GO:0010124; P:phenylacetate catabolic process; IEA:InterPro.
DR   Gene3D; 2.40.110.10; -; 1.
DR   InterPro; IPR046373; Acyl-CoA_Oxase/DH_mid-dom_sf.
DR   InterPro; IPR036250; AcylCo_DH-like_C.
DR   InterPro; IPR009100; AcylCoA_DH/oxidase_NM_dom.
DR   InterPro; IPR024677; HpaB/PvcC.
DR   InterPro; IPR004925; HpaB/PvcC/4-BUDH.
DR   InterPro; IPR024719; HpaB/PvcC/4-BUDH_C.
DR   InterPro; IPR024674; HpaB/PvcC/4-BUDH_N.
DR   InterPro; IPR012688; HpaB_gammaproteobact.
DR   PANTHER; PTHR36117; PTHR36117; 1.
DR   Pfam; PF03241; HpaB; 1.
DR   Pfam; PF11794; HpaB_N; 1.
DR   PIRSF; PIRSF500125; 4_HPA_large; 1.
DR   PIRSF; PIRSF000331; HpaA_HpaB; 1.
DR   SUPFAM; SSF47203; SSF47203; 1.
DR   SUPFAM; SSF56645; SSF56645; 1.
DR   TIGRFAMs; TIGR02310; HpaB-2; 1.
PE   2: Evidence at transcript level;
KW   Aromatic hydrocarbons catabolism; FAD; Flavoprotein; Monooxygenase;
KW   Oxidoreductase.
FT   CHAIN           1..520
FT                   /note="4-hydroxyphenylacetate 3-monooxygenase oxygenase
FT                   component"
FT                   /id="PRO_0000084033"
SQ   SEQUENCE   520 AA;  58651 MW;  0EA03932BA7121EA CRC64;
     MKPENFRADT KRPLTGEEYL KSLQDGREIY IYGERVKDVT THPAFRNAAA SVAQLYDALH
     NPELQNTLCW GTDTGSGGYT HKFFRVAKSA DDLRQQRDAI AEWSRLSYGW MGRTPDYKAA
     FGGGLGANPG FYGQFEQNAR DWYTRIQETG LYFNHAIVNP PIDRHKPADE VKDVYIKLEK
     ETDAGIIVSG AKVVATNSAL THYNMIGFGS AQVMGENPDF ALMFVAPMDA EGDKLISRAS
     YELVAGATGS PYDYPLSSRF DENDAILVMD NVLIPWENVL IYRDFDRCRR WTMEGGFARM
     YPLQACVRLA VKLDFITALL KRSLECTGTL EFRGVQAELG EVVAWRNMFW ALSDSMCAEA
     TPWVNGAYLP DHAALQTYRV MAPMPYAKIK NIIERSVTSG LIYLPSSARD LNNPQINDTL
     AKYVRGSNGM DHVERIKILK LMWDAIGSEF GGCHELYEIN YSGSQDEIRL QCLRQAQSSG
     NMDKMMAMVD RCLSEYDQNG WTVPHLHNNT DINMLDKLLK