HPAC_ECOLX
ID HPAC_ECOLX Reviewed; 170 AA.
AC Q57501;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=4-hydroxyphenylacetate 3-monooxygenase reductase component;
DE EC=1.5.1.36;
DE AltName: Full=4-HPA 3-monooxygenase small component;
DE AltName: Full=Flavin:NADH reductase;
GN Name=hpaC;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=W / ATCC 11105 / DSM 1900 / 113-3;
RX PubMed=8077235; DOI=10.1016/s0021-9258(17)31719-2;
RA Prieto M.A., Garcia J.L.;
RT "Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of
RT Escherichia coli. A two-protein component enzyme.";
RL J. Biol. Chem. 269:22823-22829(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=W / ATCC 11105 / DSM 1900 / 113-3;
RX PubMed=8550403; DOI=10.1128/jb.178.1.111-120.1996;
RA Prieto M.A., Diaz E., Garcia J.L.;
RT "Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway
RT of Escherichia coli W: engineering a mobile aromatic degradative cluster.";
RL J. Bacteriol. 178:111-120(1996).
RN [3]
RP SEQUENCE REVISION.
RC STRAIN=W / ATCC 11105 / DSM 1900 / 113-3;
RA Prieto M.A.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PROTEIN SEQUENCE OF 1-5, AND CHARACTERIZATION.
RX PubMed=10633095; DOI=10.1128/jb.182.3.627-636.2000;
RA Galan B., Diaz E., Prieto M.A., Garcia J.L.;
RT "Functional analysis of the small component of the 4-hydroxyphenylacetate
RT 3-monooxygenase of Escherichia coli W: a prototype of a new flavin:NAD(P)H
RT reductase subfamily.";
RL J. Bacteriol. 182:627-636(2000).
CC -!- FUNCTION: Catalyzes the reduction of free flavins (FMN, FAD and
CC riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the
CC large HpaB component or to other electron acceptors such as cytochrome
CC c and Fe(3+) ion.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a reduced flavin + NAD(+) = an oxidized flavin + 2 H(+) +
CC NADH; Xref=Rhea:RHEA:31303, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:60531, ChEBI:CHEBI:62787; EC=1.5.1.36;
CC -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC degradation; pyruvate and succinate semialdehyde from 4-
CC hydroxyphenylacetate: step 1/7.
CC -!- SUBUNIT: Homodimer (Probable). 4-HPA 3-monooxygenase consists of a
CC reductase component HpaC and an oxygenase component HpaB.
CC {ECO:0000305}.
CC -!- INDUCTION: By 4-hydroxyphenylacetic acid.
CC -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC HpaC subfamily. {ECO:0000305}.
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DR EMBL; Z29081; CAA82322.2; -; Genomic_DNA.
DR EMBL; Z37980; CAA86049.2; -; Genomic_DNA.
DR PIR; C55349; C55349.
DR RefSeq; WP_001175455.1; NZ_WVWF01000016.1.
DR AlphaFoldDB; Q57501; -.
DR SMR; Q57501; -.
DR STRING; 585034.ECIAI1_4566; -.
DR GeneID; 66671770; -.
DR eggNOG; COG1853; Bacteria.
DR OMA; CINVLPA; -.
DR BioCyc; MetaCyc:MON-2842; -.
DR BRENDA; 1.5.1.36; 2168.
DR SABIO-RK; Q57501; -.
DR UniPathway; UPA00208; UER00416.
DR GO; GO:0036382; F:flavin reductase (NADH) activity; IEA:UniProtKB-EC.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016651; F:oxidoreductase activity, acting on NAD(P)H; IEA:InterPro.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0042537; P:benzene-containing compound metabolic process; IEA:InterPro.
DR Gene3D; 2.30.110.10; -; 1.
DR InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR InterPro; IPR011982; HPA_mOase_red.
DR InterPro; IPR012349; Split_barrel_FMN-bd.
DR Pfam; PF01613; Flavin_Reduct; 1.
DR SMART; SM00903; Flavin_Reduct; 1.
DR TIGRFAMs; TIGR02296; HpaC; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; Direct protein sequencing; Flavoprotein;
KW FMN; NAD; Oxidoreductase.
FT CHAIN 1..170
FT /note="4-hydroxyphenylacetate 3-monooxygenase reductase
FT component"
FT /id="PRO_0000085532"
SQ SEQUENCE 170 AA; 18522 MW; 9333B3DA130A6661 CRC64;
MQLDEQRLRF RDAMASLSAA VNIITTEGDA GQCGITATAV CSVTDTPPSL MVCINANSAM
NPVFQGNGKL CVNVLNHEQE LMARHFAGMT GMAMEERFSL SCWQKGPLAQ PVLKGSLASL
EGEIRDVQAI GTHLVYLVEI KNIILSAEGH GLIYFKRRFH PVMLEMEAAI
