ID   HPAC_KLEOX              Reviewed;         170 AA.
AC   Q48441;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=4-hydroxyphenylacetate 3-monooxygenase reductase component;
DE            EC=1.5.1.36;
DE   AltName: Full=4-HPA 3-monooxygenase small component;
DE   AltName: Full=Flavin:NADH reductase;
GN   Name=hpaC; Synonyms=hpaH;
OS   Klebsiella oxytoca.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M5a1;
RX   PubMed=9133323; DOI=10.1007/s002030050429;
RA   Gibello A., Suarez M., Allende J.L., Martin M.;
RT   "Molecular cloning and analysis of the genes encoding the 4-
RT   hydroxyphenylacetate hydroxylase from Klebsiella pneumoniae.";
RL   Arch. Microbiol. 167:160-166(1997).
CC   -!- FUNCTION: Catalyzes the reduction of free flavins (FMN, FAD and
CC       riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the
CC       large HpaB component or to other electron acceptors such as cytochrome
CC       c and Fe(3+) ion (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a reduced flavin + NAD(+) = an oxidized flavin + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:31303, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60531, ChEBI:CHEBI:62787; EC=1.5.1.36;
CC   -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC       degradation; pyruvate and succinate semialdehyde from 4-
CC       hydroxyphenylacetate: step 1/7.
CC   -!- SUBUNIT: Homodimer. 4-HPA 3-monooxygenase consists of a reductase
CC       component HpaC and an oxygenase component HpaB (By similarity).
CC       {ECO:0000250}.
CC   -!- INDUCTION: By 3- or 4-hydroxyphenylacetic acid.
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       HpaC subfamily. {ECO:0000305}.
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DR   EMBL; L41068; AAC37121.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48441; -.
DR   SMR; Q48441; -.
DR   STRING; 571.MC52_11755; -.
DR   eggNOG; COG1853; Bacteria.
DR   UniPathway; UPA00208; UER00416.
DR   GO; GO:0036382; F:flavin reductase (NADH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016651; F:oxidoreductase activity, acting on NAD(P)H; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0042537; P:benzene-containing compound metabolic process; IEA:InterPro.
DR   Gene3D; 2.30.110.10; -; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR011982; HPA_mOase_red.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
DR   TIGRFAMs; TIGR02296; HpaC; 1.
PE   2: Evidence at transcript level;
KW   Aromatic hydrocarbons catabolism; Flavoprotein; FMN; NAD; Oxidoreductase.
FT   CHAIN           1..170
FT                   /note="4-hydroxyphenylacetate 3-monooxygenase reductase
FT                   component"
FT                   /id="PRO_0000085533"
SQ   SEQUENCE   170 AA;  18555 MW;  DDE7FAFE9A3FD0E5 CRC64;
     MQLDEQRLRF RDAMASLSPA VNVITTEAEA GAAVSPHRPS CSVTDTPPSV MVCINANSAM
     NPVFQGNGKL CINVLNHEQE EMARHFAGMT GMTMDDRFGL SGWQKGALGQ PVLKGALASL
     EGEISQVQTI GSHLVYLVEI RNITLSQQGH GLIYFKRRFH PVMMEMDVVA