ID   HPAC_SALDU              Reviewed;         170 AA.
AC   Q9RPU2;
DT   02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=4-hydroxyphenylacetate 3-monooxygenase reductase component;
DE            EC=1.5.1.36;
DE   AltName: Full=4-HPA 3-monooxygenase small component;
DE   AltName: Full=Flavin:NADH reductase;
GN   Name=hpaC;
OS   Salmonella dublin.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=98360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2229;
RA   Galyov E.E., Wood M.W., Hedges S.;
RT   "Characterization of the hpa genetic locus from Salmonella dublin.";
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reduction of free flavins (FMN, FAD and
CC       riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the
CC       large HpaB component or to other electron acceptors such as cytochrome
CC       c and Fe(3+) ion (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a reduced flavin + NAD(+) = an oxidized flavin + 2 H(+) +
CC         NADH; Xref=Rhea:RHEA:31303, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60531, ChEBI:CHEBI:62787; EC=1.5.1.36;
CC   -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC       degradation; pyruvate and succinate semialdehyde from 4-
CC       hydroxyphenylacetate: step 1/7.
CC   -!- SUBUNIT: Homodimer (Probable). 4-HPA 3-monooxygenase consists of a
CC       reductase component HpaC and an oxygenase component HpaB (By
CC       similarity). {ECO:0000250, ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the non-flavoprotein flavin reductase family.
CC       HpaC subfamily. {ECO:0000305}.
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DR   EMBL; AF144422; AAD53504.1; -; Genomic_DNA.
DR   RefSeq; WP_001195550.1; NZ_VDCP01000004.1.
DR   AlphaFoldDB; Q9RPU2; -.
DR   SMR; Q9RPU2; -.
DR   PATRIC; fig|98360.39.peg.3133; -.
DR   OMA; CINVLPA; -.
DR   UniPathway; UPA00208; UER00416.
DR   GO; GO:0036382; F:flavin reductase (NADH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016651; F:oxidoreductase activity, acting on NAD(P)H; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0042537; P:benzene-containing compound metabolic process; IEA:InterPro.
DR   Gene3D; 2.30.110.10; -; 1.
DR   InterPro; IPR002563; Flavin_Rdtase-like_dom.
DR   InterPro; IPR011982; HPA_mOase_red.
DR   InterPro; IPR012349; Split_barrel_FMN-bd.
DR   Pfam; PF01613; Flavin_Reduct; 1.
DR   SMART; SM00903; Flavin_Reduct; 1.
DR   TIGRFAMs; TIGR02296; HpaC; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Flavoprotein; FMN; NAD; Oxidoreductase.
FT   CHAIN           1..170
FT                   /note="4-hydroxyphenylacetate 3-monooxygenase reductase
FT                   component"
FT                   /id="PRO_0000085534"
SQ   SEQUENCE   170 AA;  18462 MW;  2A6F7AC5CFE82203 CRC64;
     MQVDEQRLHF RDAMASLAAA VNIVTTAGHA GRCGITATAV CSVTDTPPSV MVCINANSAM
     NPVFQGNGRL CINVLNHEQE LMARHFAGMT GMAMEERFHQ PCWQNGPLGQ PVLNGALAGL
     EGEISEVQTI GTHLVYLVAI KNIILSQDGH GLIYFKRRFH PVRLEMEAPV