HPAG_ECOLX
ID HPAG_ECOLX Reviewed; 429 AA.
AC Q46978;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase;
DE Includes:
DE RecName: Full=2-hydroxyhepta-2,4-diene-1,7-dioate isomerase;
DE Short=HHDD isomerase;
DE EC=5.3.3.10 {ECO:0000250|UniProtKB:P37352};
DE AltName: Full=5-carboxymethyl-2-hydroxymuconate Delta-isomerase;
DE Includes:
DE RecName: Full=5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase;
DE EC=4.1.1.68 {ECO:0000250|UniProtKB:P37352};
DE AltName: Full=5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase;
DE Short=OPET decarboxylase;
GN Name=hpaG;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=W / ATCC 11105 / DSM 1900 / 113-3;
RX PubMed=8550403; DOI=10.1128/jb.178.1.111-120.1996;
RA Prieto M.A., Diaz E., Garcia J.L.;
RT "Molecular characterization of the 4-hydroxyphenylacetate catabolic pathway
RT of Escherichia coli W: engineering a mobile aromatic degradative cluster.";
RL J. Bacteriol. 178:111-120(1996).
CC -!- FUNCTION: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5-tricarboxylic
CC acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7-dioate) and isomerizes it
CC to OHED (2-oxo-hept-3-ene-1,7-dioate). {ECO:0000250|UniProtKB:P37352}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E,4Z)-5-hydroxypenta-2,4-diene-1,2,5-tricarboxylate =
CC (3E,5R)-5-carboxy-2-oxohept-3-enedioate; Xref=Rhea:RHEA:18813,
CC ChEBI:CHEBI:47961, ChEBI:CHEBI:87491; EC=5.3.3.10;
CC Evidence={ECO:0000250|UniProtKB:P37352};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3E,5R)-5-carboxy-2-oxohept-3-enedioate + H(+) = (4Z)-2-
CC oxohept-4-enedioate + CO2; Xref=Rhea:RHEA:14397, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:87491, ChEBI:CHEBI:87507; EC=4.1.1.68;
CC Evidence={ECO:0000250|UniProtKB:P37352};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:P37352};
CC -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC degradation; pyruvate and succinate semialdehyde from 4-
CC hydroxyphenylacetate: step 4/7.
CC -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC degradation; pyruvate and succinate semialdehyde from 4-
CC hydroxyphenylacetate: step 5/7.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P37352}.
CC -!- SIMILARITY: Belongs to the FAH family. {ECO:0000305}.
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DR EMBL; Z37980; CAA86040.1; -; Genomic_DNA.
DR RefSeq; WP_000679083.1; NZ_WTPV01000016.1.
DR AlphaFoldDB; Q46978; -.
DR SMR; Q46978; -.
DR STRING; 585034.ECIAI1_4575; -.
DR PATRIC; fig|562.5049.peg.2807; -.
DR eggNOG; COG0179; Bacteria.
DR OMA; VMKGTIF; -.
DR BioCyc; MetaCyc:MON-604; -.
DR UniPathway; UPA00208; UER00419.
DR UniPathway; UPA00208; UER00420.
DR GO; GO:0008704; F:5-carboxymethyl-2-hydroxymuconate delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0018800; F:5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:1901023; P:4-hydroxyphenylacetate catabolic process; IEA:InterPro.
DR Gene3D; 3.90.850.10; -; 2.
DR InterPro; IPR011234; Fumarylacetoacetase-like_C.
DR InterPro; IPR036663; Fumarylacetoacetase_C_sf.
DR InterPro; IPR012684; HPA_isomer/decarb_C.
DR InterPro; IPR012686; HPA_isomer/decarb_N.
DR Pfam; PF01557; FAA_hydrolase; 2.
DR SUPFAM; SSF56529; SSF56529; 2.
DR TIGRFAMs; TIGR02303; HpaG-C-term; 1.
DR TIGRFAMs; TIGR02305; HpaG-N-term; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; Isomerase; Lyase; Metal-binding;
KW Multifunctional enzyme; Repeat.
FT CHAIN 1..429
FT /note="4-hydroxyphenylacetate degradation bifunctional
FT isomerase/decarboxylase"
FT /id="PRO_0000156833"
FT REPEAT 1..215
FT /note="Approximate"
FT REPEAT 216..429
FT /note="Approximate"
FT BINDING 276
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 278
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 307
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
SQ SEQUENCE 429 AA; 47108 MW; BC8AC5E6FEB455B1 CRC64;
MKGTIFAVAL NHRSQLDAWQ EAFQQSPYKA PPKTAVWFIK PRNTVIGCGE PIPFPQGEKV
LSGATVALIV GKTATKVREE DAAEYIAGYA LANDVSLPEE SFYRPAIKAK CRDGFCPIGE
TVALSNVDNL TIYTEINGRP ADHWNTADLQ RNAAQLLSAL SEFATLNPGD AILLGTPQAR
VEIQPGDRVR VLAEGFPPLE NPVVDEREVT TRKSFPTLPH PHGTLFALGL NYADHASELE
FKPPEEPLVF LKAPNTLTGD NQTSVRPNNI EYMHYEAELV VVIGKQARNV SEADAMDYVA
GYTVCNDYAI RDYLENYYRP NLRVKSRDGL TPMLSTIVPK EAIPDPHNLT LRTFVNGELR
QQGTTADLIF SVPFLIAYLS EFMTLNPGDM IATGTPKGLS DVVPGDEVVV EVEGVGRLVN
RIVSEETAK
