ID   HPARI_HELPZ             Reviewed;         297 AA.
AC   A0A3P7XL18;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-FEB-2019, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Alarmin release inhibitor {ECO:0000303|PubMed:29045903};
DE            Short=HpARI {ECO:0000303|PubMed:29045903};
DE   Flags: Precursor;
GN   Name=ARI {ECO:0000303|PubMed:29045903};
GN   ORFNames=HPBE_LOCUS8140 {ECO:0000312|EMBL:VDO75100.1};
OS   Heligmosomoides polygyrus (Parasitic roundworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Strongylida;
OC   Trichostrongyloidea; Heligmosomatidae; Heligmosomoides.
OX   NCBI_TaxID=6339 {ECO:0000312|EMBL:VDO75100.1};
RN   [1] {ECO:0000312|EMBL:VDO75100.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG   Pathogen Informatics;
RL   Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH MOUSE IL33,
RP   AND SUBCELLULAR LOCATION.
RX   PubMed=29045903; DOI=10.1016/j.immuni.2017.09.015;
RA   Osbourn M., Soares D.C., Vacca F., Cohen E.S., Scott I.C., Gregory W.F.,
RA   Smyth D.J., Toivakka M., Kemter A.M., le Bihan T., Wear M., Hoving D.,
RA   Filbey K.J., Hewitson J.P., Henderson H., Gonzalez-Ciscar A., Errington C.,
RA   Vermeren S., Astier A.L., Wallace W.A., Schwarze J., Ivens A.C.,
RA   Maizels R.M., McSorley H.J.;
RT   "HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33.";
RL   Immunity 47:739-751(2017).
CC   -!- FUNCTION: Secreted protein which suppresses the host allergic response
CC       by inhibiting the interaction of host IL33 with its receptor in order
CC       to maintain parasitic infection (PubMed:29045903). Binds to both host
CC       IL33 and host nuclear DNA and this dual binding blocks the interaction
CC       of IL33 with its receptor, and tethers IL33 within necrotic cells,
CC       preventing its release, and blocking allergic response initiation
CC       (PubMed:29045903). {ECO:0000269|PubMed:29045903}.
CC   -!- SUBUNIT: Interacts with mouse IL33 (in reduced form).
CC       {ECO:0000269|PubMed:29045903}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29045903}. Host
CC       nucleus {ECO:0000269|PubMed:29045903}.
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DR   EMBL; UZAH01026067; VDO75100.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3P7XL18; -.
DR   Proteomes; UP000050761; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR   GO; GO:0002113; F:interleukin-33 binding; IPI:UniProtKB.
DR   GO; GO:0060300; P:regulation of cytokine activity; IDA:UniProtKB.
DR   GO; GO:0060759; P:regulation of response to cytokine stimulus; IDA:UniProtKB.
DR   GO; GO:0052572; P:response to host immune response; IDA:UniProtKB.
DR   GO; GO:0044414; P:suppression of host defenses by symbiont; IDA:UniProtKB.
DR   InterPro; IPR035976; Sushi/SCR/CCP_sf.
DR   SUPFAM; SSF57535; SSF57535; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; DNA-binding; Glycoprotein; Host nucleus; Secreted; Signal;
KW   Sushi.
FT   SIGNAL          1..?
FT                   /evidence="ECO:0000305"
FT   CHAIN           ?..297
FT                   /note="Alarmin release inhibitor"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000448485"
FT   DOMAIN          151..211
FT                   /note="Sushi"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT   CARBOHYD        107
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        175
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        190
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   DISULFID        183..209
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
SQ   SEQUENCE   297 AA;  32955 MW;  E34783345F0CC54A CRC64;
     MDIIGQVYCF TGAPHTITCL LREQFINWYI KYKRSENYPA FRELVVMYRL FLALGFLTFI
     NAAGQRCRFT DVERDKGYTG MLLKGRLRKT AGNGRTVELI CGRGNHNYTC ESGVLKESSP
     RQARCGCKGI LEMLFDMPKE ERPSPMYDSV TYDPTPNTPT TVGKDGIWNG VDYRNGSTVK
     PYCDTGPVIN GSSKAVCVSG KWVPTLGVCP KMCSIGSLKE NGKFVDVTAT TKGDELNPPP
     REQTLIPIVR KVDKDKVQHG VKVVALCKAE DSTTAAEGVQ EFECDNGKWK PEPVPCP