ID   HPCB_ECOLX              Reviewed;         276 AA.
AC   Q05353;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=3,4-dihydroxyphenylacetate 2,3-dioxygenase;
DE            EC=1.13.11.15;
DE   AltName: Full=Homoprotocatechuate 2,3-dioxygenase;
DE            Short=HPC dioxygenase;
GN   Name=hpcB;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-22.
RC   STRAIN=C;
RX   PubMed=2261999; DOI=10.1016/0014-5793(90)81437-s;
RA   Roper D.I., Cooper R.A.;
RT   "Subcloning and nucleotide sequence of the 3,4-dihydroxyphenylacetate
RT   (homoprotocatechuate) 2,3-dioxygenase gene from Escherichia coli C.";
RL   FEBS Lett. 275:53-57(1990).
CC   -!- FUNCTION: Transforms homoprotocatechuic acid (HPC) into 5-
CC       carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3,4-dihydroxyphenylacetate + O2 = 2-hydroxy-5-
CC         carboxymethylmuconate semialdehyde + H(+); Xref=Rhea:RHEA:15633,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17612,
CC         ChEBI:CHEBI:58030; EC=1.13.11.15;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC   -!- PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate
CC       degradation; pyruvate and succinate semialdehyde from 4-
CC       hydroxyphenylacetate: step 2/7.
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DR   EMBL; X55200; CAA38985.1; -; Genomic_DNA.
DR   PIR; S12962; S12962.
DR   AlphaFoldDB; Q05353; -.
DR   SMR; Q05353; -.
DR   STRING; 585034.ECIAI1_4573; -.
DR   KEGG; ag:CAA38985; -.
DR   eggNOG; COG3384; Bacteria.
DR   UniPathway; UPA00208; UER00417.
DR   GO; GO:0008687; F:3,4-dihydroxyphenylacetate 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd07370; HPCD; 1.
DR   InterPro; IPR011984; HPCD.
DR   InterPro; IPR004183; Xdiol_dOase_suB.
DR   Pfam; PF02900; LigB; 1.
DR   TIGRFAMs; TIGR02298; HpaD_Fe; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing;
KW   Iron; Oxidoreductase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2261999"
FT   CHAIN           2..276
FT                   /note="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
FT                   /id="PRO_0000084040"
SQ   SEQUENCE   276 AA;  31495 MW;  7EDCFC4A228C17CE CRC64;
     MGKLALAAKI THVPSMYLSE LPGKNHGCRQ GAIDGHKEIS KRCREMGVDT IIVFDTHWLV
     NSAYHINCAD HFEGVYTSNE LPHFIRDMTY NYEGNPELGQ LIADEALKLG VRAKAHNIPS
     LKLEYGSVVP MRYMNEDKRF KVVSISAFCT VHDFADSRKL GERIVKAIEQ YDGTVAVLAS
     GSLSHRFIDD QRAEEGMNSY TREFDRQMDE RVVKLWREGQ FKEFCNMLPE YADYCYGEGN
     MHDTVMLLGM LGWDKYDGKV WSLSPSYSQA SWHRSG