HPCL4_HUMAN
ID HPCL4_HUMAN Reviewed; 191 AA.
AC Q9UM19; B2R5U2; D3DPU1; Q5TG97; Q8N611;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Hippocalcin-like protein 4;
DE Short=HLP4;
GN Name=HPCAL4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=10520747; DOI=10.3109/10425179809072192;
RA Kobayashi M., Sakai E., Furuta Y., Takamatsu K.;
RT "Isolation of two human cDNAs, HLP3 and HLP4, homologous to the neuron-
RT specific calcium-binding protein genes.";
RL DNA Seq. 9:171-176(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Amygdala;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May be involved in the calcium-dependent regulation of
CC rhodopsin phosphorylation. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9UM19; Q7Z3H0-1: ANKRD33; NbExp=3; IntAct=EBI-744820, EBI-16746154;
CC Q9UM19; P55087: AQP4; NbExp=3; IntAct=EBI-744820, EBI-10104898;
CC Q9UM19; P40199: CEACAM6; NbExp=3; IntAct=EBI-744820, EBI-4314501;
CC Q9UM19; Q86UW9: DTX2; NbExp=7; IntAct=EBI-744820, EBI-740376;
CC Q9UM19; P13726: F3; NbExp=3; IntAct=EBI-744820, EBI-1040727;
CC Q9UM19; Q96AQ9: FAM131C; NbExp=6; IntAct=EBI-744820, EBI-741921;
CC Q9UM19; Q99732: LITAF; NbExp=6; IntAct=EBI-744820, EBI-725647;
CC Q9UM19; Q9UJ68: MSRA; NbExp=3; IntAct=EBI-744820, EBI-19157918;
CC Q9UM19; Q96GD3: SCMH1; NbExp=3; IntAct=EBI-744820, EBI-713793;
CC Q9UM19; Q7Z699: SPRED1; NbExp=6; IntAct=EBI-744820, EBI-5235340;
CC Q9UM19; Q9BXU2: TEX13B; NbExp=3; IntAct=EBI-744820, EBI-12007066;
CC Q9UM19; Q9NZQ8: TRPM5; NbExp=3; IntAct=EBI-744820, EBI-18161658;
CC Q9UM19; Q9H892-2: TTC12; NbExp=3; IntAct=EBI-744820, EBI-10274410;
CC Q9UM19; A0A1U9X8X8; NbExp=3; IntAct=EBI-744820, EBI-17234977;
CC Q9UM19; Q7DB77: tir; Xeno; NbExp=3; IntAct=EBI-744820, EBI-6480811;
CC -!- MISCELLANEOUS: Probably binds two or three calcium ions. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the recoverin family. {ECO:0000305}.
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DR EMBL; AB001105; BAA86892.1; -; mRNA.
DR EMBL; AL136591; CAB66526.1; -; mRNA.
DR EMBL; AK312314; BAG35239.1; -; mRNA.
DR EMBL; AL035404; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471059; EAX07257.1; -; Genomic_DNA.
DR EMBL; CH471059; EAX07258.1; -; Genomic_DNA.
DR EMBL; BC030827; AAH30827.1; -; mRNA.
DR CCDS; CCDS441.1; -.
DR RefSeq; NP_001269325.1; NM_001282396.1.
DR RefSeq; NP_057341.1; NM_016257.3.
DR RefSeq; XP_016856936.1; XM_017001447.1.
DR AlphaFoldDB; Q9UM19; -.
DR SMR; Q9UM19; -.
DR BioGRID; 119542; 28.
DR IntAct; Q9UM19; 22.
DR MINT; Q9UM19; -.
DR STRING; 9606.ENSP00000361935; -.
DR iPTMnet; Q9UM19; -.
DR PhosphoSitePlus; Q9UM19; -.
DR BioMuta; HPCAL4; -.
DR DMDM; 51316542; -.
DR EPD; Q9UM19; -.
DR jPOST; Q9UM19; -.
DR MassIVE; Q9UM19; -.
DR MaxQB; Q9UM19; -.
DR PaxDb; Q9UM19; -.
DR PeptideAtlas; Q9UM19; -.
DR PRIDE; Q9UM19; -.
DR ProteomicsDB; 85171; -.
DR Antibodypedia; 31935; 161 antibodies from 21 providers.
DR DNASU; 51440; -.
DR Ensembl; ENST00000372844.8; ENSP00000361935.3; ENSG00000116983.13.
DR Ensembl; ENST00000617690.2; ENSP00000481834.1; ENSG00000116983.13.
DR GeneID; 51440; -.
DR KEGG; hsa:51440; -.
DR MANE-Select; ENST00000372844.8; ENSP00000361935.3; NM_016257.4; NP_057341.1.
DR UCSC; uc001cdr.5; human.
DR CTD; 51440; -.
DR DisGeNET; 51440; -.
DR GeneCards; HPCAL4; -.
DR HGNC; HGNC:18212; HPCAL4.
DR HPA; ENSG00000116983; Tissue enriched (brain).
DR MIM; 619211; gene.
DR neXtProt; NX_Q9UM19; -.
DR OpenTargets; ENSG00000116983; -.
DR PharmGKB; PA134924885; -.
DR VEuPathDB; HostDB:ENSG00000116983; -.
DR eggNOG; KOG0044; Eukaryota.
DR GeneTree; ENSGT00940000161166; -.
DR HOGENOM; CLU_072366_1_0_1; -.
DR InParanoid; Q9UM19; -.
DR OMA; QQLYIKX; -.
DR OrthoDB; 1369072at2759; -.
DR PhylomeDB; Q9UM19; -.
DR TreeFam; TF300009; -.
DR PathwayCommons; Q9UM19; -.
DR SignaLink; Q9UM19; -.
DR BioGRID-ORCS; 51440; 11 hits in 1068 CRISPR screens.
DR ChiTaRS; HPCAL4; human.
DR GeneWiki; HPCAL4; -.
DR GenomeRNAi; 51440; -.
DR Pharos; Q9UM19; Tdark.
DR PRO; PR:Q9UM19; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9UM19; protein.
DR Bgee; ENSG00000116983; Expressed in right frontal lobe and 128 other tissues.
DR ExpressionAtlas; Q9UM19; baseline and differential.
DR Genevisible; Q9UM19; HS.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:Ensembl.
DR GO; GO:0005509; F:calcium ion binding; TAS:ProtInc.
DR GO; GO:0008022; F:protein C-terminus binding; IEA:Ensembl.
DR GO; GO:0019904; F:protein domain specific binding; IEA:Ensembl.
DR GO; GO:0007417; P:central nervous system development; TAS:ProtInc.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR028846; Recoverin.
DR InterPro; IPR029534; VILIP-2.
DR PANTHER; PTHR23055; PTHR23055; 1.
DR PANTHER; PTHR23055:SF84; PTHR23055:SF84; 1.
DR Pfam; PF00036; EF-hand_1; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Lipoprotein; Metal-binding; Myristate; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..191
FT /note="Hippocalcin-like protein 4"
FT /id="PRO_0000073777"
FT DOMAIN 24..59
FT /note="EF-hand 1"
FT /evidence="ECO:0000305"
FT DOMAIN 60..95
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 96..131
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 146..181
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 73
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 75
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 77
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 79
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 84
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 109
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 111
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 113
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 115
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 120
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 159
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 161
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 163
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 165
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT CONFLICT 179
FT /note="P -> A (in Ref. 6; AAH30827)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 191 AA; 22202 MW; F705065604A8A38D CRC64;
MGKTNSKLAP EVLEDLVQNT EFSEQELKQW YKGFLKDCPS GILNLEEFQQ LYIKFFPYGD
ASKFAQHAFR TFDKNGDGTI DFREFICALS VTSRGSFEQK LNWAFEMYDL DGDGRITRLE
MLEIIEAIYK MVGTVIMMRM NQDGLTPQQR VDKIFKKMDQ DKDDQITLEE FKEAAKSDPS
IVLLLQCDMQ K
