HPE_HAEI3
ID HPE_HAEI3 Reviewed; 160 AA.
AC A4NBN9;
DT 13-NOV-2013, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2007, sequence version 1.
DT 25-MAY-2022, entry version 28.
DE RecName: Full=Surface-adhesin protein E;
DE Flags: Precursor;
GN Name=pe; ORFNames=CGSHi3655_04936;
OS Haemophilus influenzae (strain NTHi 3655).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=375177;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NTHi 3655;
RX PubMed=17550610; DOI=10.1186/gb-2007-8-6-r103;
RA Hogg J.S., Hu F.Z., Janto B., Boissy R., Hayes J., Keefe R., Post J.C.,
RA Ehrlich G.D.;
RT "Characterization and modeling of the Haemophilus influenzae core and
RT supragenomes based on the complete genomic sequences of Rd and 12 clinical
RT nontypeable strains.";
RL Genome Biol. 8:R103.1-R103.18(2007).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=NTHi 3655, and NTHi 772;
RX PubMed=18069033; DOI=10.1016/j.micinf.2007.10.006;
RA Ronander E., Brant M., Janson H., Sheldon J., Forsgren A., Riesbeck K.;
RT "Identification of a novel Haemophilus influenzae protein important for
RT adhesion to epithelial cells.";
RL Microbes Infect. 10:87-96(2008).
RN [3]
RP FUNCTION, INTERACTION WITH VITRONECTIN, AND DISRUPTION PHENOTYPE.
RC STRAIN=NTHi 3655;
RX PubMed=19635912; DOI=10.4049/jimmunol.0803226;
RA Hallstrom T., Blom A.M., Zipfel P.F., Riesbeck K.;
RT "Nontypeable Haemophilus influenzae protein E binds vitronectin and is
RT important for serum resistance.";
RL J. Immunol. 183:2593-2601(2009).
RN [4]
RP FUNCTION.
RC STRAIN=NTHi 3655;
RX PubMed=19125675; DOI=10.1086/596211;
RA Ronander E., Brant M., Eriksson E., Morgelin M., Hallgren O.,
RA Westergren-Thorsson G., Forsgren A., Riesbeck K.;
RT "Nontypeable Haemophilus influenzae adhesin protein E: characterization and
RT biological activity.";
RL J. Infect. Dis. 199:522-531(2009).
RN [5]
RP FUNCTION, INTERACTION WITH LAMININ AND VITRONECTIN, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=NTHi 3655;
RX PubMed=21881122; DOI=10.1093/infdis/jir459;
RA Hallstrom T., Singh B., Resman F., Blom A.M., Morgelin M., Riesbeck K.;
RT "Haemophilus influenzae protein E binds to the extracellular matrix by
RT concurrently interacting with laminin and vitronectin.";
RL J. Infect. Dis. 204:1065-1074(2011).
RN [6]
RP FUNCTION, INTERACTION WITH VITRONECTIN, AND MUTAGENESIS OF LYS-85 AND
RP ARG-86.
RC STRAIN=NTHi 3655;
RX PubMed=21542857; DOI=10.1111/j.1365-2958.2011.07678.x;
RA Singh B., Jalalvand F., Morgelin M., Zipfel P., Blom A.M., Riesbeck K.;
RT "Haemophilus influenzae protein E recognizes the C-terminal domain of
RT vitronectin and modulates the membrane attack complex.";
RL Mol. Microbiol. 81:80-98(2011).
RN [7]
RP FUNCTION, INTERACTION WITH PLASMINOGEN, AND DISRUPTION PHENOTYPE.
RC STRAIN=NTHi 3655;
RX PubMed=22124123; DOI=10.4049/jimmunol.1101927;
RA Barthel D., Singh B., Riesbeck K., Zipfel P.F.;
RT "Haemophilus influenzae uses the surface protein E to acquire human
RT plasminogen and to evade innate immunity.";
RL J. Immunol. 188:379-385(2012).
RN [8]
RP CRYSTALLIZATION, AND SUBUNIT.
RX PubMed=22298005; DOI=10.1107/s1744309111055503;
RA Singh B., Al Jubair T., Fornvik K., Thunnissen M.M., Riesbeck K.;
RT "Crystallization and X-ray diffraction analysis of a novel surface-adhesin
RT protein: protein E from Haemophilus influenzae.";
RL Acta Crystallogr. F 68:222-226(2012).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 26-157, AND SUBUNIT.
RX PubMed=23275089; DOI=10.1128/iai.01111-12;
RA Singh B., Al-Jubair T., Morgelin M., Thunnissen M.M., Riesbeck K.;
RT "The unique structure of Haemophilus influenzae protein E reveals multiple
RT binding sites for host factors.";
RL Infect. Immun. 81:801-814(2013).
CC -!- FUNCTION: Acts as a multifunctional adhesin involved in direct
CC interactions with host epithelial cells and host proteins, including
CC vitronectin, laminin and plasminogen. In addition, interaction with
CC serum vitronectin plays an important role in bacterial serum
CC resistance, and conversion of plasminogen to plasmin at the cell
CC surface aids in immune evasion and contributes to bacterial virulence.
CC Induces a pro-inflammatory epithelial cell response, leading to
CC interleukin-8 (IL-8) secretion and up-regulation of ICAM1.
CC {ECO:0000269|PubMed:18069033, ECO:0000269|PubMed:19125675,
CC ECO:0000269|PubMed:19635912, ECO:0000269|PubMed:21542857,
CC ECO:0000269|PubMed:21881122, ECO:0000269|PubMed:22124123}.
CC -!- SUBUNIT: Homodimer. Interacts with host vitronectin, laminin and
CC plasminogen. Can interact with both immobilized and soluble
CC vitronectin. {ECO:0000269|PubMed:19635912, ECO:0000269|PubMed:21542857,
CC ECO:0000269|PubMed:21881122, ECO:0000269|PubMed:22124123,
CC ECO:0000269|PubMed:22298005, ECO:0000269|PubMed:23275089}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000269|PubMed:18069033}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:18069033}. Cell surface
CC {ECO:0000269|PubMed:18069033}.
CC -!- DISRUPTION PHENOTYPE: Mutant displays a reduced binding to vitronectin,
CC laminin, plasminogen and epithelial cells, and is more sensitive to
CC killing by human serum compared with the wild type.
CC {ECO:0000269|PubMed:18069033, ECO:0000269|PubMed:19635912,
CC ECO:0000269|PubMed:21881122, ECO:0000269|PubMed:22124123}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAZF01000008; EDJ92417.1; -; Genomic_DNA.
DR RefSeq; WP_005658162.1; NZ_AAZF01000008.1.
DR PDB; 3ZH5; X-ray; 1.80 A; A/B=26-157.
DR PDB; 3ZH6; X-ray; 2.29 A; A/B=26-157.
DR PDB; 3ZH7; X-ray; 2.10 A; A/B=29-153.
DR PDBsum; 3ZH5; -.
DR PDBsum; 3ZH6; -.
DR PDBsum; 3ZH7; -.
DR AlphaFoldDB; A4NBN9; -.
DR SMR; A4NBN9; -.
DR IntAct; A4NBN9; 1.
DR EnsemblBacteria; EDJ92417; EDJ92417; CGSHi3655_04936.
DR Proteomes; UP000003185; Unassembled WGS sequence.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR Gene3D; 2.40.128.710; -; 1.
DR InterPro; IPR043088; Adhesin_E.
DR InterPro; IPR031939; Adhesin_E-like.
DR InterPro; IPR016595; Adhesin_E_Pasteurellaceae.
DR Pfam; PF16747; Adhesin_E; 1.
DR PIRSF; PIRSF012320; Prplsmic_HI0178_prd; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW Signal; Virulence.
FT SIGNAL 1..15
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 16..160
FT /note="Surface-adhesin protein E"
FT /id="PRO_0000424421"
FT REGION 41..68
FT /note="Interaction with laminin and plasminogen"
FT REGION 84..108
FT /note="Interaction with vitronectin and epithelial cells"
FT LIPID 16
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 16
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT MUTAGEN 85
FT /note="K->E: 50% reduction in binding to vitronectin. Does
FT not bind vitronectin; when associated with D-86."
FT /evidence="ECO:0000269|PubMed:21542857"
FT MUTAGEN 86
FT /note="R->D: 48% reduction in binding to vitronectin. Does
FT not bind vitronectin; when associated with E-85."
FT /evidence="ECO:0000269|PubMed:21542857"
SQ SEQUENCE 160 AA; 18350 MW; D6F1115CD7C5DD00 CRC64;
MKKIILTLSL GLLTACSAQI QKAEQNDMKL APPTDVRSGY IRLVKNVNYY IDSESIWVDN
QEPQIVHFDA VVNLDKGLYV YPEPKRYARS VRQYKILNCA NYHLTQVRTD FYDEFWGQGL
RAAPKKQKKH TLSLTPDTTL YNAAQIICAN YGKAFSVDKK