ID   HPF_BRADU               Reviewed;         203 AA.
AC   P30334;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE            Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
GN   Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839}; OrderedLocusNames=blr0724;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=USDA 110spc4;
RX   PubMed=1991712; DOI=10.1128/jb.173.3.1125-1138.1991;
RA   Kullik I., Fritsche S., Knobel H., Sanjuan J., Hennecke H., Fischer H.-M.;
RT   "Bradyrhizobium japonicum has two differentially regulated, functional
RT   homologs of the sigma 54 gene (rpoN).";
RL   J. Bacteriol. 173:1125-1138(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC       ribosomes in stationary phase; 100S ribosomes are translationally
CC       inactive and sometimes present during exponential growth.
CC       {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SUBUNIT: Interacts with 100S ribosomes. {ECO:0000255|HAMAP-
CC       Rule:MF_00839}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC       Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
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DR   EMBL; M59243; AAA26242.1; -; Genomic_DNA.
DR   EMBL; BA000040; BAC45989.1; -; Genomic_DNA.
DR   PIR; C38179; C38179.
DR   RefSeq; NP_767364.1; NC_004463.1.
DR   RefSeq; WP_011083549.1; NZ_CP011360.1.
DR   AlphaFoldDB; P30334; -.
DR   SMR; P30334; -.
DR   STRING; 224911.27348973; -.
DR   EnsemblBacteria; BAC45989; BAC45989; BAC45989.
DR   GeneID; 64020588; -.
DR   KEGG; bja:blr0724; -.
DR   PATRIC; fig|224911.44.peg.96; -.
DR   eggNOG; COG1544; Bacteria.
DR   HOGENOM; CLU_071472_0_1_5; -.
DR   InParanoid; P30334; -.
DR   OMA; RRLKDHH; -.
DR   PhylomeDB; P30334; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR   GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR   GO; GO:0045900; P:negative regulation of translational elongation; IBA:GO_Central.
DR   GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR   CDD; cd00552; RaiA; 1.
DR   Gene3D; 3.30.160.100; -; 1.
DR   Gene3D; 3.30.505.50; -; 1.
DR   HAMAP; MF_00839; HPF; 1.
DR   InterPro; IPR034694; HPF_long/plastid.
DR   InterPro; IPR036567; RHF-like.
DR   InterPro; IPR003489; RHF/RaiA.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
DR   Pfam; PF02482; Ribosomal_S30AE; 1.
DR   SUPFAM; SSF69754; SSF69754; 1.
DR   TIGRFAMs; TIGR00741; yfiA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Translation regulation.
FT   CHAIN           1..203
FT                   /note="Ribosome hibernation promotion factor"
FT                   /id="PRO_0000097416"
SQ   SEQUENCE   203 AA;  21626 MW;  CC379B27062E990B CRC64;
     MTLRISGKSV SVGEALRGRV SDRTEEVLRK YFDGNYSGHI TLSKDGFGFR TDCALHLDSG
     ITLEADSNAP DAYASADQAL VMIEKRLKRY KSRLKDRSAR KAHVASAALA AMDATSYVLE
     APGEGEDEDE VTGYSPVIIA EATTSLKQLS VSEAVMELDL SGAPCLVFQH GSSGRVNIIY
     RRADGNVGWV DPPGGKADGK AGG