ID   HPF_LACLM               Reviewed;         185 AA.
AC   A2RIX0;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839, ECO:0000303|PubMed:24279750};
DE            Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
DE   AltName: Full=Lactococcus lactis hibernation promotion factor {ECO:0000303|PubMed:24279750};
DE            Short=LIHPF {ECO:0000303|PubMed:24279750};
GN   Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839, ECO:0000305};
GN   Synonyms=yfiA {ECO:0000303|PubMed:24279750}; OrderedLocusNames=llmg_0616;
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
RN   [2]
RP   FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MG1363;
RX   PubMed=24279750; DOI=10.1111/mmi.12468;
RA   Puri P., Eckhardt T.H., Franken L.E., Fusetti F., Stuart M.C.,
RA   Boekema E.J., Kuipers O.P., Kok J., Poolman B.;
RT   "Lactococcus lactis YfiA is necessary and sufficient for ribosome
RT   dimerization.";
RL   Mol. Microbiol. 91:394-407(2014).
CC   -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC       ribosomes in stationary phase; 100S ribosomes are translationally
CC       inactive and sometimes present during exponential growth. Able to
CC       dimerize E.coli 70S ribosomes in vitro (PubMed:24279750).
CC       {ECO:0000255|HAMAP-Rule:MF_00839, ECO:0000269|PubMed:24279750}.
CC   -!- SUBUNIT: Interacts with 100S ribosomes in stationary phase; alters the
CC       relative position of the 30S and 50S subunits. {ECO:0000255|HAMAP-
CC       Rule:MF_00839, ECO:0000269|PubMed:24279750}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- DOMAIN: The N-terminus (residues 1-126) is homologous to YaiA and HPF
CC       of E.coli and can associate with ribosomes, while the C-terminus
CC       (residues 126-185) seems to be a functional paralog of E.coli RMF.
CC       {ECO:0000269|PubMed:24279750}.
CC   -!- DISRUPTION PHENOTYPE: No visible effect on growth until cells have been
CC       starved for 5 days; cells recover from starvation less well than wild-
CC       type and are dead after 14 days starvation. No 100S ribosomes are
CC       formed even in stationary phase. {ECO:0000269|PubMed:24279750}.
CC   -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC       Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
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DR   EMBL; AM406671; CAL97217.1; -; Genomic_DNA.
DR   RefSeq; WP_011834629.1; NZ_WJVF01000050.1.
DR   PDB; 5MYJ; EM; 5.60 A; A=1-185.
DR   PDBsum; 5MYJ; -.
DR   AlphaFoldDB; A2RIX0; -.
DR   SMR; A2RIX0; -.
DR   STRING; 416870.llmg_0616; -.
DR   PRIDE; A2RIX0; -.
DR   EnsemblBacteria; CAL97217; CAL97217; llmg_0616.
DR   GeneID; 61108917; -.
DR   KEGG; llm:llmg_0616; -.
DR   eggNOG; COG1544; Bacteria.
DR   HOGENOM; CLU_071472_0_3_9; -.
DR   OMA; HGGYGVI; -.
DR   PhylomeDB; A2RIX0; -.
DR   BioCyc; LLAC416870:LLMG_RS03210-MON; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00552; RaiA; 1.
DR   Gene3D; 3.30.160.100; -; 1.
DR   Gene3D; 3.30.505.50; -; 1.
DR   HAMAP; MF_00839; HPF; 1.
DR   InterPro; IPR034694; HPF_long/plastid.
DR   InterPro; IPR036567; RHF-like.
DR   InterPro; IPR003489; RHF/RaiA.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
DR   Pfam; PF02482; Ribosomal_S30AE; 1.
DR   SUPFAM; SSF69754; SSF69754; 1.
DR   TIGRFAMs; TIGR00741; yfiA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Translation regulation.
FT   CHAIN           1..185
FT                   /note="Ribosome hibernation promotion factor"
FT                   /id="PRO_0000436444"
FT   REGION          1..125
FT                   /note="Probably still associates with ribosome"
FT                   /evidence="ECO:0000305|PubMed:24279750"
FT   REGION          126..185
FT                   /note="Required but not sufficient to restore ribosome
FT                   dimerization, in vitro will replace E.coli RMF in ribosome
FT                   dimerization"
FT                   /evidence="ECO:0000269|PubMed:24279750"
SQ   SEQUENCE   185 AA;  21314 MW;  9FAABAAFE0B5F0EA CRC64;
     MIKFNIRGEN VEVTDAIRAY VEDKIGKLDK YFNDGHEVTA YVNLKVYTEK RAKVEVTLPA
     KNVTLRAEDT SQDMYSSIDF VEEKLERQIR KYKTRMNRKP RNAVPTGQVF GDEFAPLDTT
     DEVAEDHVDI VRTKHVALKP MDAEEAVLQM DMLGHDFYVF TDADSNGTHV VYRRTDGRYG
     LIETE