ID   HPF_LISM4               Reviewed;         187 AA.
AC   A0A0H3GEZ8;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE            Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839, ECO:0000303|PubMed:25422304};
DE   AltName: Full=Ribosome hibernation-promoting factor {ECO:0000303|PubMed:25422304};
GN   Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839, ECO:0000303|PubMed:25422304};
GN   OrderedLocusNames=LMRG_01737;
OS   Listeria monocytogenes serotype 1/2a (strain 10403S).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=393133;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=10403S;
RG   The Broad Institute Genome Sequencing Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M.,
RA   Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D.,
RA   Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L.,
RA   Arachchi H.M., Berlin A., Borenstein D., Brown A., Chapman S.B., Chen Z.,
RA   Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J.,
RA   Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L.,
RA   Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D.,
RA   Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T.,
RA   Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C.,
RA   Haas B., Nusbaum C., Birren B.;
RT   "The genome sequence of Listeria monocytogenes strain 10403S.";
RL   Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=10403S;
RX   PubMed=25422304; DOI=10.1128/jb.02223-14;
RA   Kline B.C., McKay S.L., Tang W.W., Portnoy D.A.;
RT   "The Listeria monocytogenes hibernation-promoting factor is required for
RT   the formation of 100S ribosomes, optimal fitness, and pathogenesis.";
RL   J. Bacteriol. 197:581-591(2015).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=10403S;
RX   PubMed=26324267; DOI=10.1128/aac.01532-15;
RA   McKay S.L., Portnoy D.A.;
RT   "Ribosome hibernation facilitates tolerance of stationary-phase bacteria to
RT   aminoglycosides.";
RL   Antimicrob. Agents Chemother. 59:6992-6999(2015).
CC   -!- FUNCTION: Involved in 100S ribosome formation from 70S ribosomes; 100S
CC       ribosomes are probably translationally inactive (PubMed:25422304).
CC       Ribosome hibernation may be used by the cell to decrease overall energy
CC       consumption under nutrient-limiting conditions (PubMed:26324267).
CC       Unlike E.coli, 100S ribosomes are present from mid-exponential growth,
CC       peak during the transition from log to stationary phase and then
CC       decrease (PubMed:25422304). {ECO:0000269|PubMed:25422304,
CC       ECO:0000305|PubMed:26324267}.
CC   -!- SUBUNIT: Interacts with 100S ribosomes (PubMed:25422304).
CC       {ECO:0000255|HAMAP-Rule:MF_00839, ECO:0000269|PubMed:25422304}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839,
CC       ECO:0000305|PubMed:25422304}.
CC   -!- INDUCTION: Transcribed early in log-phase growth, peaks at log to
CC       stationary phase transition (PubMed:25422304). Repressed by growth in
CC       the presence of glucose, which also decreases 100S ribosome content
CC       (PubMed:25422304). Induced by growth in ethanol, NaCl, and 46 degrees
CC       Celsius; stress respones are under control of the sigma-B factor (sigB)
CC       (PubMed:25422304). {ECO:0000269|PubMed:25422304}.
CC   -!- DISRUPTION PHENOTYPE: Loss of 100S ribosomes. Shows no change in growth
CC       in pure culture after 13 days, however performs very poorly (about
CC       1000-fold decrease) in competition with wild-type (PubMed:25422304).
CC       About 5-fold less virulent than wild-type upon oral or tail vein
CC       injection of BALB/c mice (PubMed:25422304). Sensitive to aminoglycoside
CC       antibiotics in stationary phase; sensitivity is suppressed by
CC       transcription and translation inhibitors or by disrupting the proton
CC       motive force (PubMed:26324267). Stationary phase cells have 5-fold
CC       higher than normal levels of ATP and NADH and accumulates higher
CC       intracellular levels of gentamicin (PubMed:26324267). Aminoglycosides
CC       such as gentamicin bind to ribosomes and interfere with translation.
CC       {ECO:0000269|PubMed:25422304, ECO:0000269|PubMed:26324267}.
CC   -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC       Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839,
CC       ECO:0000305|PubMed:25422304}.
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DR   EMBL; CP002002; AEO07487.1; -; Genomic_DNA.
DR   RefSeq; WP_003722643.1; NC_017544.1.
DR   AlphaFoldDB; A0A0H3GEZ8; -.
DR   SMR; A0A0H3GEZ8; -.
DR   EnsemblBacteria; AEO07487; AEO07487; LMRG_01737.
DR   GeneID; 61171769; -.
DR   GeneID; 66486197; -.
DR   KEGG; lmt:LMRG_01737; -.
DR   HOGENOM; CLU_071472_0_3_9; -.
DR   OMA; HGGYGVI; -.
DR   Proteomes; UP000001288; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00552; RaiA; 1.
DR   Gene3D; 3.30.160.100; -; 1.
DR   Gene3D; 3.30.505.50; -; 1.
DR   HAMAP; MF_00839; HPF; 1.
DR   InterPro; IPR034694; HPF_long/plastid.
DR   InterPro; IPR036567; RHF-like.
DR   InterPro; IPR003489; RHF/RaiA.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
DR   Pfam; PF02482; Ribosomal_S30AE; 1.
DR   SUPFAM; SSF69754; SSF69754; 1.
DR   TIGRFAMs; TIGR00741; yfiA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Stress response; Translation regulation.
FT   CHAIN           1..187
FT                   /note="Ribosome hibernation promotion factor"
FT                   /id="PRO_0000436445"
SQ   SEQUENCE   187 AA;  21633 MW;  C04015A32DDE15D5 CRC64;
     MLKYNIRGEN IEVTEPIRDY VEKKIDKLER YFTETPDANV HVNLKVYSDK NAKVEVTIPL
     PNLVLRAEET SGDLYASIDL IVDKLERQIR KHKTKVNRKF RDKGAERDYF AYSDVNGSTP
     PEENEGDFDL EIVRTKQFSL KPMDSEEAVL QMNLLGHSFY VYTDAETNGT NIVYSRKDGK
     YGLIETN