ID   HPF_MYCTU               Reviewed;         219 AA.
AC   O05886; F2GKB7; I6X6S6; L0TER5;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2016, sequence version 4.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE            Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
GN   Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839}; OrderedLocusNames=Rv3241c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   PROTEIN SEQUENCE OF 4-16, AND SEQUENCE REVISION TO N-TERMINUS.
RC   STRAIN=H37Rv;
RX   PubMed=34915127; DOI=10.1016/j.ygeno.2021.12.001;
RA   Shi J., Meng S., Wan L., Zhang Z., Jiang S., Zhu H., Dai E., Chang L.,
RA   Gao H., Wan K., Zhang L., Zhao X., Liu H., Lyu Z., Zhang Y., Xu P.;
RT   "Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct
RT   annotated encoding genes.";
RL   Genomics 114:292-304(2021).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC       ribosomes in stationary phase; 100S ribosomes are translationally
CC       inactive and sometimes present during exponential growth.
CC       {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SUBUNIT: Interacts with 100S ribosomes. {ECO:0000255|HAMAP-
CC       Rule:MF_00839}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC       Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP46060.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
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DR   EMBL; AL123456; CCP46060.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_217758.3; NC_000962.3.
DR   AlphaFoldDB; O05886; -.
DR   SMR; O05886; -.
DR   STRING; 83332.Rv3241c; -.
DR   PaxDb; O05886; -.
DR   PRIDE; O05886; -.
DR   DNASU; 888849; -.
DR   GeneID; 888849; -.
DR   KEGG; mtu:Rv3241c; -.
DR   PATRIC; fig|83332.12.peg.3624; -.
DR   TubercuList; Rv3241c; -.
DR   eggNOG; COG1544; Bacteria.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR   GO; GO:0043024; F:ribosomal small subunit binding; IBA:GO_Central.
DR   GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR   GO; GO:0045900; P:negative regulation of translational elongation; IBA:GO_Central.
DR   GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR   CDD; cd00552; RaiA; 1.
DR   Gene3D; 3.30.160.100; -; 1.
DR   Gene3D; 3.30.505.50; -; 1.
DR   HAMAP; MF_00839; HPF; 1.
DR   InterPro; IPR034694; HPF_long/plastid.
DR   InterPro; IPR036567; RHF-like.
DR   InterPro; IPR003489; RHF/RaiA.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
DR   Pfam; PF02482; Ribosomal_S30AE; 1.
DR   SUPFAM; SSF69754; SSF69754; 1.
DR   TIGRFAMs; TIGR00741; yfiA; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Reference proteome;
KW   Translation regulation.
FT   CHAIN           1..219
FT                   /note="Ribosome hibernation promotion factor"
FT                   /id="PRO_0000436446"
SQ   SEQUENCE   219 AA;  25093 MW;  CC6E9B0D0B2A0854 CRC64;
     MSRLAVDSGQ VLAEPKSNAE IVFKGRNVEI PDHFRIYVSQ KLARLERFDR TIYLFDVELD
     HERNRRQRKS CQRVEITARG RGPVVRGEAC ADSFYAALES AVVKLESRLR RGKDRRKVHY
     GDKTPVSLAE ATAVVPAPEN GFNTRPAEAH DHDGAVVERE PGRIVRTKEH PAKPMSVDDA
     LYQMELVGHD FFLFYDKDTE RPSVVYRRHA YDYGLIRLA