ID   HPF_STACT               Reviewed;         188 AA.
AC   P47995; B9DJM1;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE            Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
GN   Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839}; OrderedLocusNames=Sca_0400;
OS   Staphylococcus carnosus (strain TM300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=396513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TM300;
RX   PubMed=19060169; DOI=10.1128/aem.01982-08;
RA   Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C.,
RA   Goetz F.;
RT   "Genome analysis of the meat starter culture bacterium Staphylococcus
RT   carnosus TM300.";
RL   Appl. Environ. Microbiol. 75:811-822(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 28-188.
RX   PubMed=7557338; DOI=10.1016/0378-1097(95)00267-9;
RA   Klein M., Meens J., Freudl R.;
RT   "Functional characterization of the Staphylococcus carnosus SecA protein in
RT   Escherichia coli and Bacillus subtilis secA mutant strains.";
RL   FEMS Microbiol. Lett. 131:271-277(1995).
CC   -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC       ribosomes in stationary phase; 100S ribosomes are translationally
CC       inactive and sometimes present during exponential growth.
CC       {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SUBUNIT: Interacts with 100S ribosomes. {ECO:0000255|HAMAP-
CC       Rule:MF_00839}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC       Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA56161.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AM295250; CAL27314.1; -; Genomic_DNA.
DR   EMBL; X79725; CAA56161.1; ALT_FRAME; Genomic_DNA.
DR   PIR; S47148; S47148.
DR   RefSeq; WP_015899659.1; NC_012121.1.
DR   AlphaFoldDB; P47995; -.
DR   SMR; P47995; -.
DR   STRING; 396513.SCA_0400; -.
DR   PRIDE; P47995; -.
DR   GeneID; 60545903; -.
DR   KEGG; sca:SCA_0400; -.
DR   eggNOG; COG1544; Bacteria.
DR   HOGENOM; CLU_071472_0_3_9; -.
DR   OMA; HGGYGVI; -.
DR   OrthoDB; 1766263at2; -.
DR   BioCyc; SCAR396513:SCA_RS02040-MON; -.
DR   Proteomes; UP000000444; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00552; RaiA; 1.
DR   Gene3D; 3.30.160.100; -; 1.
DR   Gene3D; 3.30.505.50; -; 1.
DR   HAMAP; MF_00839; HPF; 1.
DR   InterPro; IPR034694; HPF_long/plastid.
DR   InterPro; IPR036567; RHF-like.
DR   InterPro; IPR003489; RHF/RaiA.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
DR   Pfam; PF02482; Ribosomal_S30AE; 1.
DR   SUPFAM; SSF69754; SSF69754; 1.
DR   TIGRFAMs; TIGR00741; yfiA; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Translation regulation.
FT   CHAIN           1..188
FT                   /note="Ribosome hibernation promotion factor"
FT                   /id="PRO_0000208591"
FT   REGION          93..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        52
FT                   /note="Missing (in Ref. 2; CAA56161)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        74
FT                   /note="L -> I (in Ref. 2; CAA56161)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        80
FT                   /note="L -> K (in Ref. 2; CAA56161)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        87
FT                   /note="R -> C (in Ref. 2; CAA56161)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        156
FT                   /note="H -> T (in Ref. 2; CAA56161)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        188
FT                   /note="N -> KLICDI (in Ref. 2; CAA56161)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   188 AA;  21886 MW;  25E12963AB332A5F CRC64;
     MIRFEIHGDN LTITDAIRNY IEDKVGKLER YFTNVPNVNA HVKVKTYANS STKIEVTIPL
     NDVTLRAEER NDDLYAGIDL ITNKLERQVR KYKTRVNRKK RKESEHEPFP ATPETPPETA
     VDHDKDDEIE IIRSKQFSLK PMDSEEAVLQ MDLLGHDFFI FNDRETDGTS IVYRRKDGKY
     GLIETVEN