ID   HPF_STRP6               Reviewed;         182 AA.
AC   Q5XAQ7; P82482;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE            Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
DE   AltName: Full=Ribosome-associated factor Y {ECO:0000303|Ref.3};
GN   Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839}; OrderedLocusNames=M6_Spy1371;
OS   Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=286636;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-946 / MGAS10394;
RX   PubMed=15272401; DOI=10.1086/422697;
RA   Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E.,
RA   Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.;
RT   "Progress toward characterization of the group A Streptococcus metagenome:
RT   complete genome sequence of a macrolide-resistant serotype M6 strain.";
RL   J. Infect. Dis. 190:727-738(2004).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 1-24; 31-87; 102-129 AND 132-182.
RC   STRAIN=JRS4 / Serotype M6 {ECO:0000269|PubMed:15272401};
RA   Hogan D.A., Du P., Stevenson T.I., Whitton M., Kilby G.W., Rogers J.,
RA   VanBogelen R.A.;
RT   "Two-dimensional gel electrophoresis map of Streptococcus pyogenes
RT   proteins.";
RL   Submitted (MAY-2000) to UniProtKB.
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 126-182.
RA   Seetharaman J., Neely H., Forouhar F., Wang D., Janjua H., Cunningham K.,
RA   Owens L., Xiao R., Liu J., Baran M.C., Acton T.B., Montelione G.T.,
RA   Hunt J.F., Tong L.;
RT   "Crystal structure of a domain of ribosome-associated factor Y from
RT   Streptococcus pyogenes serotype M6. Northeast structural genomics
RT   consortium target id DR64A.";
RL   Submitted (FEB-2010) to the PDB data bank.
CC   -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC       ribosomes in stationary phase; 100S ribosomes are translationally
CC       inactive and sometimes present during exponential growth.
CC       {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SUBUNIT: Interacts with 100S ribosomes. {ECO:0000255|HAMAP-
CC       Rule:MF_00839}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC   -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC       Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
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DR   EMBL; CP000003; AAT87506.1; -; Genomic_DNA.
DR   RefSeq; WP_002988974.1; NC_006086.1.
DR   PDB; 3LYV; X-ray; 2.70 A; A/B/C/D/E/F=126-182.
DR   PDBsum; 3LYV; -.
DR   AlphaFoldDB; Q5XAQ7; -.
DR   SMR; Q5XAQ7; -.
DR   EnsemblBacteria; AAT87506; AAT87506; M6_Spy1371.
DR   KEGG; spa:M6_Spy1371; -.
DR   HOGENOM; CLU_071472_0_3_9; -.
DR   OMA; HGGYGVI; -.
DR   EvolutionaryTrace; Q5XAQ7; -.
DR   Proteomes; UP000001167; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00552; RaiA; 1.
DR   Gene3D; 3.30.160.100; -; 1.
DR   Gene3D; 3.30.505.50; -; 1.
DR   HAMAP; MF_00839; HPF; 1.
DR   InterPro; IPR034694; HPF_long/plastid.
DR   InterPro; IPR036567; RHF-like.
DR   InterPro; IPR003489; RHF/RaiA.
DR   InterPro; IPR032528; Ribosom_S30AE_C.
DR   InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR   Pfam; PF16321; Ribosom_S30AE_C; 1.
DR   Pfam; PF02482; Ribosomal_S30AE; 1.
DR   SUPFAM; SSF69754; SSF69754; 1.
DR   TIGRFAMs; TIGR00741; yfiA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Direct protein sequencing; Translation regulation.
FT   CHAIN           1..182
FT                   /note="Ribosome hibernation promotion factor"
FT                   /id="PRO_0000259418"
FT   HELIX           140..148
FT                   /evidence="ECO:0007829|PDB:3LYV"
FT   STRAND          153..159
FT                   /evidence="ECO:0007829|PDB:3LYV"
FT   TURN            160..162
FT                   /evidence="ECO:0007829|PDB:3LYV"
FT   STRAND          164..170
FT                   /evidence="ECO:0007829|PDB:3LYV"
FT   STRAND          174..181
FT                   /evidence="ECO:0007829|PDB:3LYV"
SQ   SEQUENCE   182 AA;  21100 MW;  90EFE4679B47E3F8 CRC64;
     MIKFSIRGEN IEVTEAIRDY VESKLTKIEK YFAKDQEIDA RVNLKVYRER SSKVEVTIPL
     DSVTLRAEDV SQDMYGSIDL VVDKIERQIR KNKTKIAKKH REKVPTGQVF TTEFEAEEVD
     EIPEVQVVRT KNVTLKPMDV EEARLQMELL GHDFFIYTDS EDGATNILYR REDGNLGLIE
     AK