HPF_SYNP2
ID HPF_SYNP2 Reviewed; 193 AA.
AC P47908; B1XMT6;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Ribosome hibernation promotion factor {ECO:0000255|HAMAP-Rule:MF_00839};
DE Short=HPF {ECO:0000255|HAMAP-Rule:MF_00839};
DE AltName: Full=Dark-response protein 21 {ECO:0000303|PubMed:8063707};
DE Short=DRP-21 {ECO:0000303|PubMed:8063707};
DE AltName: Full=Light-repressed protein A {ECO:0000303|PubMed:8063707};
GN Name=hpf {ECO:0000255|HAMAP-Rule:MF_00839};
GN Synonyms=lrtA {ECO:0000303|PubMed:8063707};
GN OrderedLocusNames=SYNPCC7002_A0263;
OS Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS quadruplicatum).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC unclassified Synechococcus.
OX NCBI_TaxID=32049;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=8063707; DOI=10.1016/s0021-9258(17)31907-5;
RA Tan X., Varughese M., Widger W.R.;
RT "A light-repressed transcript found in Synechococcus PCC 7002 is similar to
RT a chloroplast-specific small subunit ribosomal protein and to a
RT transcription modulator protein associated with sigma 54.";
RL J. Biol. Chem. 269:20905-20912(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT "Complete sequence of Synechococcus sp. PCC 7002.";
RL Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP INDUCTION, AND PUTATIVE FUNCTION.
RC STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RX PubMed=9576792; DOI=10.1104/pp.117.1.225;
RA Samartzidou H., Widger W.R.;
RT "Transcriptional and posttranscriptional control of mRNA from lrtA, a
RT light-repressed transcript in Synechococcus sp. PCC 7002.";
RL Plant Physiol. 117:225-234(1998).
CC -!- FUNCTION: Might modulate either transcription and/or translation.
CC {ECO:0000305|PubMed:9576792}.
CC -!- FUNCTION: Required for dimerization of active 70S ribosomes into 100S
CC ribosomes in stationary phase; 100S ribosomes are translationally
CC inactive and sometimes present during exponential growth.
CC {ECO:0000255|HAMAP-Rule:MF_00839}.
CC -!- SUBUNIT: Interacts with 100S ribosomes. {ECO:0000255|HAMAP-
CC Rule:MF_00839}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00839}.
CC -!- INDUCTION: Is repressed by light, since the transcript is synthesized
CC in the dark, but upon 20 minutes of illumination, transcript levels
CC fall below detectable limits. This light-repressed transcript is
CC present only in dark-adapted cells. Is rapidly translated at the onset
CC of illumination; in fact, is one of the first proteins to be
CC synthesized upon illumination of dark-adapted cells. Repression of the
CC transcript in the light requires protein synthesis, which suggests that
CC a specific protein is directly involved in lrtA light repression.
CC {ECO:0000269|PubMed:8063707, ECO:0000269|PubMed:9576792}.
CC -!- DISRUPTION PHENOTYPE: Knockout mutants grown in continuous light appear
CC to be the same as wild-type, showing no differences in growth or
CC photosynthetic activity. {ECO:0000269|PubMed:8063707}.
CC -!- MISCELLANEOUS: The transcript for lrtA is more stable in the dark than
CC in the light: a 3-hour half-life is estimated in the dark, whereas a 4-
CC minute half-life is observed in the light.
CC -!- SIMILARITY: Belongs to the HPF/YfiA ribosome-associated protein family.
CC Long HPF subfamily. {ECO:0000255|HAMAP-Rule:MF_00839}.
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DR EMBL; L29194; AAA62586.1; -; Genomic_DNA.
DR EMBL; CP000951; ACA98273.1; -; Genomic_DNA.
DR PIR; A53914; A53914.
DR RefSeq; WP_012305897.1; NC_010475.1.
DR AlphaFoldDB; P47908; -.
DR STRING; 32049.SYNPCC7002_A0263; -.
DR PRIDE; P47908; -.
DR EnsemblBacteria; ACA98273; ACA98273; SYNPCC7002_A0263.
DR KEGG; syp:SYNPCC7002_A0263; -.
DR eggNOG; COG1544; Bacteria.
DR HOGENOM; CLU_071472_0_2_3; -.
DR OMA; HGGYGVI; -.
DR Proteomes; UP000001688; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0044238; P:primary metabolic process; IEA:InterPro.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-UniRule.
DR CDD; cd00552; RaiA; 1.
DR Gene3D; 3.30.160.100; -; 1.
DR Gene3D; 3.30.505.50; -; 1.
DR HAMAP; MF_00839; HPF; 1.
DR InterPro; IPR034694; HPF_long/plastid.
DR InterPro; IPR036567; RHF-like.
DR InterPro; IPR003489; RHF/RaiA.
DR InterPro; IPR032528; Ribosom_S30AE_C.
DR InterPro; IPR038416; Ribosom_S30AE_C_sf.
DR Pfam; PF16321; Ribosom_S30AE_C; 1.
DR Pfam; PF02482; Ribosomal_S30AE; 1.
DR SUPFAM; SSF69754; SSF69754; 1.
DR TIGRFAMs; TIGR00741; yfiA; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Translation regulation.
FT CHAIN 1..193
FT /note="Ribosome hibernation promotion factor"
FT /id="PRO_0000208587"
FT CONFLICT 122..131
FT /note="LIGDRAPELP -> SLRSRPRAS (in Ref. 1; AAA62586)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="E -> Q (in Ref. 1; AAA62586)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 193 AA; 21911 MW; 6521B45874951D98 CRC64;
MKLLIQGNNI AVTESIHDYV ESKLEKATKH FQTFATKVDV HLSVANNARI TDKHKAEVTV
YANGTVIRAQ EGSENLYASI DLVSDKIARQ LRKYKEKNFG KKTHVQEKTS EVLPEDPVPD
NLIGDRAPEL PSEVVRMKYF AMPPMTIDEA LEQLQLVDHD FYMFLNKDTN AINVIYIRNH
GGYGVIQPRL GKE
