ID   HPI_DEIRD               Reviewed;        1036 AA.
AC   P13126;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Hexagonally packed intermediate-layer surface protein;
DE   Flags: Precursor;
GN   Name=hpi;
OS   Deinococcus radiodurans.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC   Deinococcus.
OX   NCBI_TaxID=1299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=Sark;
RX   PubMed=3667529; DOI=10.1128/jb.169.11.5216-5223.1987;
RA   Peters J., Peters M., Lottspeich F., Schaefer W., Baumeister W.;
RT   "Nucleotide sequence analysis of the gene encoding the Deinococcus
RT   radiodurans surface protein, derived amino acid sequence, and complementary
RT   protein chemical studies.";
RL   J. Bacteriol. 169:5216-5223(1987).
CC   -!- FUNCTION: Shape maintenance, possible protection from noxious enzymes
CC       or exogenous and unsettling DNA, and may mediate homotypic cell-cell
CC       contacts.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall, S-layer. Note=This bacterium
CC       is covered by a S-layer with hexagonal symmetry.
CC   -!- PTM: Glycosylated; contains six glycans.
CC   -!- PTM: Acylated in the N-terminal region.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- MISCELLANEOUS: The hydrophilic C-terminal region rich in aromatic amino
CC       acids could be engaged in interactions with nucleic acids, and the
CC       bound fatty acids and the N-terminal region could serve to anchor the
CC       layer to the outer membrane of D.radiodurans. The HPI layer contains
CC       about 30% beta structure and virtually no alpha helix.
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DR   EMBL; M17895; AAA23335.1; -; Genomic_DNA.
DR   PIR; A29832; A29832.
DR   AlphaFoldDB; P13126; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030115; C:S-layer; IEA:UniProtKB-SubCell.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR013783; Ig-like_fold.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Cell wall biogenesis/degradation; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Lipoprotein; S-layer; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           18..1036
FT                   /note="Hexagonally packed intermediate-layer surface
FT                   protein"
FT                   /id="PRO_0000032624"
FT   DISULFID        74..86
FT                   /evidence="ECO:0000269|PubMed:3667529"
FT   DISULFID        256..275
FT                   /evidence="ECO:0000305"
FT   DISULFID        642..754
FT                   /evidence="ECO:0000269|PubMed:3667529"
SQ   SEQUENCE   1036 AA;  108028 MW;  AAFF98206A74AEEE CRC64;
     MKKNIALMAL TGVLTLASCG QNGNTPTADT TAPTVSLSVN NANLPSGVGS VVLSGTVNEA
     STVVVKNNAG TTVCTVEVAA SGTFTCPATT IAGNTSTTST STSYTATATD AAKNVGTSSV
     VTVNVAGVSN PAPTTAVLTI DLAGVSSAPI TIKDANGNVV QGYDNVTVND NATITVARGV
     YTVTAGNVSG FNGPTTNFRV DLSGGNQTVT LNYTQAGTTT PTPVGSINIL TPAVGTSVTG
     GSTVRVTFDK ANEVQCMVGG AAAVTAQVDS TSGYCDVVVP NSTGNVVITV MGKGVNGQTV
     TATRNISVTQ AAVSYGVVTP AGDQELTLTS EGIVRDADSG WRRLGQGVST PSDPNLNLDI
     YIKGTVNFSV NAPAGQKVEL FLARTTGSDV PTNDDIQAGD VLRSVASTSG TETFSLDSRR
     LAEFDGVRKW IVVRINGTQV TYQPVIADNK GPQQPDPELN GVQNAYSNIL NNYNNSGLTY
     VRGPVNVFTS NPSLQDREFG QAPVGSSFVQ RRPAGFESIR YYLVPESAFN NKALQESDEM
     LRAKAVKSVA TVVSAPVLEP GTVKATSFSR VIGSGATSTV APKALDNVTY RVYAISRDQV
     GNETASATYD LVRFDNVGPT ITGSVIRDTS DLPFPSQEPE RCLSDIATIS LGGIADNVGG
     VGLNPGQGLT FTLGGRQIQA GQFDTNQLAD PEYTIGFNSL TDALGNPVVT APTNAKVYID
     NTDPTVNFNR AVMQGTYASG GRVSVESDAS DGGCGVYETR LFWDTANGVV DDATTTPAIG
     HPVQFARQRV TDGAKADSLN AGWNALQLPN GAGAVYLRSL VVDRAGNATI STTSIVVNAK
     ITNQARPLLG GFDAFKRNAS AQFVGDDNVI AGVNGTAATP NVTGNSALDN ILSLDSVGTL
     TTNAYLPRGA TETAITEKIR NVGAYGRFDA TQWNLIRDYQ LNTDPTLRSA YVNAGNLANQ
     RGNNWRIRTP WVELGSSDTA NTQQKFDFNS DLLNDFYYGR TFGNNHSVNL FSYDQFNGVV
     SDTAGAYSFY GETVRK