ID   HPLN1_HORSE             Reviewed;         354 AA.
AC   Q28381;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Hyaluronan and proteoglycan link protein 1;
DE   AltName: Full=Cartilage-linking protein 1;
DE            Short=Cartilage-link protein;
DE   AltName: Full=Proteoglycan link protein;
DE   Flags: Precursor;
GN   Name=HAPLN1; Synonyms=CTRL1;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Cartilage;
RX   PubMed=7574168;
RA   Dudhia J., Platt D.;
RT   "Complete primary sequence of equine cartilage link protein deduced from
RT   complementary DNA.";
RL   Am. J. Vet. Res. 56:959-965(1995).
CC   -!- FUNCTION: Stabilizes the aggregates of proteoglycan monomers with
CC       hyaluronic acid in the extracellular cartilage matrix. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HAPLN family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X78077; CAA54987.1; -; mRNA.
DR   PIR; S42938; S42938.
DR   RefSeq; NP_001075973.1; NM_001082504.1.
DR   AlphaFoldDB; Q28381; -.
DR   SMR; Q28381; -.
DR   STRING; 9796.ENSECAP00000014153; -.
DR   PaxDb; Q28381; -.
DR   PRIDE; Q28381; -.
DR   Ensembl; ENSECAT00000017421; ENSECAP00000014153; ENSECAG00000016477.
DR   GeneID; 100034208; -.
DR   KEGG; ecb:100034208; -.
DR   CTD; 1404; -.
DR   VGNC; VGNC:18691; HAPLN1.
DR   GeneTree; ENSGT00940000159267; -.
DR   HOGENOM; CLU_052285_1_0_1; -.
DR   InParanoid; Q28381; -.
DR   OMA; NYQGRVF; -.
DR   OrthoDB; 743812at2759; -.
DR   TreeFam; TF332134; -.
DR   Proteomes; UP000002281; Chromosome 14.
DR   Bgee; ENSECAG00000016477; Expressed in articular cartilage of joint and 12 other tissues.
DR   GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0045202; C:synapse; IEA:Ensembl.
DR   GO; GO:0005540; F:hyaluronic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR   GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR   GO; GO:0001501; P:skeletal system development; IBA:GO_Central.
DR   Gene3D; 2.60.40.10; -; 1.
DR   Gene3D; 3.10.100.10; -; 2.
DR   InterPro; IPR016186; C-type_lectin-like/link_sf.
DR   InterPro; IPR016187; CTDL_fold.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR003599; Ig_sub.
DR   InterPro; IPR013106; Ig_V-set.
DR   InterPro; IPR000538; Link_dom.
DR   Pfam; PF07686; V-set; 1.
DR   Pfam; PF00193; Xlink; 2.
DR   PRINTS; PR01265; LINKMODULE.
DR   SMART; SM00409; IG; 1.
DR   SMART; SM00406; IGv; 1.
DR   SMART; SM00445; LINK; 2.
DR   SUPFAM; SSF48726; SSF48726; 1.
DR   SUPFAM; SSF56436; SSF56436; 2.
DR   PROSITE; PS50835; IG_LIKE; 1.
DR   PROSITE; PS01241; LINK_1; 2.
DR   PROSITE; PS50963; LINK_2; 2.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Extracellular matrix; Glycoprotein; Hyaluronic acid;
KW   Immunoglobulin domain; Reference proteome; Repeat; Secreted.
FT   PROPEP          1..15
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000013175"
FT   CHAIN           16..354
FT                   /note="Hyaluronan and proteoglycan link protein 1"
FT                   /id="PRO_0000013176"
FT   DOMAIN          38..152
FT                   /note="Ig-like V-type"
FT   DOMAIN          159..254
FT                   /note="Link 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00323"
FT   DOMAIN          259..351
FT                   /note="Link 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00323"
FT   CARBOHYD        21
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        56
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        61..139
FT                   /evidence="ECO:0000250"
FT   DISULFID        181..252
FT                   /evidence="ECO:0000250"
FT   DISULFID        205..226
FT                   /evidence="ECO:0000250"
FT   DISULFID        279..349
FT                   /evidence="ECO:0000250"
FT   DISULFID        304..325
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   354 AA;  40077 MW;  D815CBB7816C7172 CRC64;
     MKSLLLLVLI SICGADHRSD NYTLDHDRVI HIQAENGPRL LVEAEQAKVF SHRGGNVTLP
     CKFLRDPTAF GSGTHKIRIK WTKLTSDYLK EVDVFVSMGY HKKTYGGYQG RVFLKGGSDN
     DASLVITDLT LDDYGRYKCE VIEGLEDDTA VVALDLQGVV FPYFPRLGRY NLNFHEAQQA
     CLDQDAVIAS FDQLYDAWRG GLDWCNAGWL SDGSVQYPIT KPREPCGGQN TVPGVRNYGF
     WDKEKSRYDV FCFTSNFNGR FYYLIHPTKL TYDEAVQACL KDGAQIAKVG QIFAAWKLLG
     YDRCDAGWLA DGSVRYPISR PRRRCSPTEA AVRFVGFPDK KHKLYGVYCF RAYN