ID   3SUC1_NAJME             Reviewed;          65 AA.
AC   P0DQQ3;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=Muscarinic toxin-like protein Tx-NM3-2 {ECO:0000303|PubMed:33672715, ECO:0000305};
OS   Naja melanoleuca (Forest cobra) (Black-lipped cobra).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Naja.
OX   NCBI_TaxID=8643;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=33672715; DOI=10.3390/toxins13020164;
RA   Son L., Kryukova E., Ziganshin R., Andreeva T., Kudryavtsev D.,
RA   Kasheverov I., Tsetlin V., Utkin Y.;
RT   "Novel three-finger neurotoxins from Naja melanoleuca cobra venom interact
RT   with GABAA and nicotinic acetylcholine receptors.";
RL   Toxins 13:0-0(2021).
CC   -!- FUNCTION: Binds weakly to the muscarinic acetylcholine receptor (CHRM)
CC       (By similarity). Does not show inhibition on GABA(A) receptors
CC       (PubMed:33672715). {ECO:0000250|UniProtKB:P82462,
CC       ECO:0000269|PubMed:33672715}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P82462}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P82462}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000250|UniProtKB:P82462}.
CC   -!- MASS SPECTROMETRY: Mass=7441.50; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:33672715};
CC   -!- MISCELLANEOUS: Is classified as a P-type cytotoxin, since a proline
CC       residue stands at position 33 (Pro-31 in standard classification).
CC       {ECO:0000250|UniProtKB:P82462}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. Type C muscarinic toxin sub-subfamily. {ECO:0000305}.
CC   -!- CAUTION: Has been partially sequenced. The missing C-terminal residues
CC       are assumed to be the same as in the orthologs and are therefore
CC       indicated in the sequence. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P0DQQ3; -.
DR   SMR; P0DQQ3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   CDD; cd00206; snake_toxin; 1.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR003571; Snake_3FTx.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   InterPro; IPR018354; Snake_toxin_con_site.
DR   InterPro; IPR035076; Toxin/TOLIP.
DR   Pfam; PF00087; Toxin_TOLIP; 1.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond;
KW   G-protein coupled acetylcholine receptor impairing toxin;
KW   G-protein coupled receptor impairing toxin; Neurotoxin;
KW   Postsynaptic neurotoxin; Secreted; Toxin.
FT   CHAIN           1..65
FT                   /note="Muscarinic toxin-like protein Tx-NM3-2"
FT                   /evidence="ECO:0000269|PubMed:33672715"
FT                   /id="PRO_0000453026"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        17..42
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        46..57
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        58..63
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   UNSURE          63..65
FT                   /note="Assigned by comparison with orthologs"
FT                   /evidence="ECO:0000250|UniProtKB:P82462"
SQ   SEQUENCE   65 AA;  7366 MW;  1388A82AE2327351 CRC64;
     LICVKEKFLF SETTETCPDG QNVCFNQAHL IYPGKYKRTR GCAATCPKLQ NRDVIFCCST
     DKCNL