HPLN1_RAT
ID HPLN1_RAT Reviewed; 354 AA.
AC P03994;
DT 23-OCT-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Hyaluronan and proteoglycan link protein 1;
DE AltName: Full=Cartilage-linking protein 1;
DE Short=Cartilage-link protein;
DE AltName: Full=Proteoglycan link protein;
DE Flags: Precursor;
GN Name=Hapln1; Synonyms=Crtl1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2452158; DOI=10.1016/s0021-9258(18)68749-6;
RA Rhodes C., Doege K., Sasaki M., Yamada Y.;
RT "Alternative splicing generates two different mRNA species for rat link
RT protein.";
RL J. Biol. Chem. 263:6063-6067(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 126-354.
RX PubMed=3459153; DOI=10.1073/pnas.83.11.3761;
RA Doege K., Hassell J.R., Caterson B., Yamada Y.;
RT "Link protein cDNA sequence reveals a tandemly repeated protein
RT structure.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:3761-3765(1986).
RN [3]
RP PROTEIN SEQUENCE OF 16-354.
RX PubMed=2419334; DOI=10.1016/s0021-9258(17)35678-8;
RA Neame P.J., Christner J.E., Baker J.R.;
RT "The primary structure of link protein from rat chondrosarcoma proteoglycan
RT aggregate.";
RL J. Biol. Chem. 261:3519-3535(1986).
CC -!- FUNCTION: Stabilizes the aggregates of proteoglycan monomers with
CC hyaluronic acid in the extracellular cartilage matrix.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=Long;
CC IsoId=P03994-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P03994-2; Sequence=VSP_005301, VSP_005302;
CC -!- SIMILARITY: Belongs to the HAPLN family. {ECO:0000305}.
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DR EMBL; M22340; AAA41535.1; -; Genomic_DNA.
DR EMBL; M22336; AAA41535.1; JOINED; Genomic_DNA.
DR EMBL; M22337; AAA41535.1; JOINED; Genomic_DNA.
DR EMBL; M22339; AAA41535.1; JOINED; Genomic_DNA.
DR EMBL; M22340; AAA41536.1; -; Genomic_DNA.
DR EMBL; M22336; AAA41536.1; JOINED; Genomic_DNA.
DR EMBL; M22338; AAA41536.1; JOINED; Genomic_DNA.
DR EMBL; M22339; AAA41536.1; JOINED; Genomic_DNA.
DR PIR; A28654; LKRT2.
DR RefSeq; XP_006231810.1; XM_006231748.3. [P03994-1]
DR AlphaFoldDB; P03994; -.
DR SMR; P03994; -.
DR BioGRID; 247990; 2.
DR IntAct; P03994; 2.
DR MINT; P03994; -.
DR STRING; 10116.ENSRNOP00000045959; -.
DR GlyGen; P03994; 1 site.
DR PaxDb; P03994; -.
DR PRIDE; P03994; -.
DR Ensembl; ENSRNOT00000042958; ENSRNOP00000045959; ENSRNOG00000032002. [P03994-1]
DR Ensembl; ENSRNOT00000108518; ENSRNOP00000094436; ENSRNOG00000032002. [P03994-2]
DR GeneID; 29331; -.
DR UCSC; RGD:2412; rat. [P03994-1]
DR CTD; 1404; -.
DR RGD; 2412; Hapln1.
DR eggNOG; ENOG502QRAR; Eukaryota.
DR GeneTree; ENSGT00940000159267; -.
DR HOGENOM; CLU_052285_1_0_1; -.
DR InParanoid; P03994; -.
DR OMA; NYQGRVF; -.
DR PhylomeDB; P03994; -.
DR TreeFam; TF332134; -.
DR Reactome; R-RNO-3000178; ECM proteoglycans.
DR PRO; PR:P03994; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000032002; Expressed in frontal cortex and 4 other tissues.
DR Genevisible; P03994; RN.
DR GO; GO:0031012; C:extracellular matrix; ISO:RGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0045202; C:synapse; ISO:RGD.
DR GO; GO:0005540; F:hyaluronic acid binding; TAS:RGD.
DR GO; GO:0005198; F:structural molecule activity; TAS:RGD.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR GO; GO:0001501; P:skeletal system development; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.10.100.10; -; 2.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR000538; Link_dom.
DR Pfam; PF07686; V-set; 1.
DR Pfam; PF00193; Xlink; 2.
DR PRINTS; PR01265; LINKMODULE.
DR SMART; SM00409; IG; 1.
DR SMART; SM00406; IGv; 1.
DR SMART; SM00445; LINK; 2.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF56436; SSF56436; 2.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS01241; LINK_1; 2.
DR PROSITE; PS50963; LINK_2; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Disulfide bond;
KW Extracellular matrix; Glycoprotein; Hyaluronic acid; Immunoglobulin domain;
KW Reference proteome; Repeat; Secreted.
FT PROPEP 1..9
FT /evidence="ECO:0000250"
FT /id="PRO_0000013183"
FT CHAIN 10..354
FT /note="Hyaluronan and proteoglycan link protein 1"
FT /id="PRO_0000013184"
FT DOMAIN 38..152
FT /note="Ig-like V-type"
FT DOMAIN 159..254
FT /note="Link 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00323"
FT DOMAIN 259..351
FT /note="Link 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00323"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT DISULFID 61..139
FT /evidence="ECO:0000269|PubMed:2419334"
FT DISULFID 181..252
FT /evidence="ECO:0000269|PubMed:2419334"
FT DISULFID 205..226
FT /evidence="ECO:0000269|PubMed:2419334"
FT DISULFID 279..349
FT /evidence="ECO:0000269|PubMed:2419334"
FT DISULFID 304..325
FT /evidence="ECO:0000269|PubMed:2419334"
FT VAR_SEQ 34..88
FT /note="AENGPRLLVEAEQAKVFSHRGGNVTLPCKFYRDPTAFGSGIHKIRIKWTKLT
FT SDY -> DCTAFWKLIRGRQRSSASPVGILTMPCCFPWRKHYTWKGIKSLKLPSLAISD
FT RTS (in isoform Short)"
FT /evidence="ECO:0000305"
FT /id="VSP_005301"
FT VAR_SEQ 89..158
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000305"
FT /id="VSP_005302"
FT CONFLICT 322
FT /note="R -> W (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 40262 MW; 23278AEA56273D6C CRC64;
MRSLLFLVLI SVCRADHLSD SYTPDQDRVI HIQAENGPRL LVEAEQAKVF SHRGGNVTLP
CKFYRDPTAF GSGIHKIRIK WTKLTSDYLR EVDVFVSMGY HKKTYGGYQG RVFLKGGSDN
DASLIITDLT LEDYGRYKCE VIEGLEDDTA VVALELQGVV FPYFPRLGRY NLNFHEARQA
CLDQDAVIAS FDQLYDAWRG GLDWCNAGWL SDGSVQYPIT KPREPCGGQN TVPGVRNYGF
WDKDKSRYDV FCFTSNFNGR FYYLIHPTKL TYDEAVQACL NDGAQIAKVG QIFAAWKLLG
YDRCDAGWLA DGSVRYPISR PRRRCSPTEA AVRFVGFPDK KHKLYGVYCF RAYN
