HPLN2_HUMAN
ID HPLN2_HUMAN Reviewed; 340 AA.
AC Q9GZV7; Q5T3J0;
DT 16-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Hyaluronan and proteoglycan link protein 2;
DE AltName: Full=Brain link protein 1;
DE Flags: Precursor;
GN Name=HAPLN2; Synonyms=BRAL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=11027579; DOI=10.1006/bbrc.2000.3583;
RA Hirakawa S., Oohashi T., Su W.-D., Yoshioka H., Murakami T., Arata J.,
RA Ninomiya Y.;
RT "The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and
RT characterization as a novel link protein expressed in adult brain.";
RL Biochem. Biophys. Res. Commun. 276:982-989(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mediates a firm binding of versican V2 to hyaluronic acid.
CC May play a pivotal role in the formation of the hyaluronan-associated
CC matrix in the central nervous system (CNS) which facilitates neuronal
CC conduction and general structural stabilization. Binds to hyaluronic
CC acid (By similarity). {ECO:0000250|UniProtKB:Q9ESM3}.
CC -!- INTERACTION:
CC Q9GZV7; Q8WXK3-2: ASB13; NbExp=3; IntAct=EBI-11956675, EBI-12015080;
CC Q9GZV7; Q8N9N5-2: BANP; NbExp=3; IntAct=EBI-11956675, EBI-11524452;
CC Q9GZV7; Q86Z20: CCDC125; NbExp=3; IntAct=EBI-11956675, EBI-11977221;
CC Q9GZV7; Q9UHD4: CIDEB; NbExp=3; IntAct=EBI-11956675, EBI-7062247;
CC Q9GZV7; Q96B26: EXOSC8; NbExp=3; IntAct=EBI-11956675, EBI-371922;
CC Q9GZV7; Q13643: FHL3; NbExp=3; IntAct=EBI-11956675, EBI-741101;
CC Q9GZV7; Q5TD97: FHL5; NbExp=3; IntAct=EBI-11956675, EBI-750641;
CC Q9GZV7; Q53EP0-3: FNDC3B; NbExp=3; IntAct=EBI-11956675, EBI-10242151;
CC Q9GZV7; A6NEM1: GOLGA6L9; NbExp=3; IntAct=EBI-11956675, EBI-5916454;
CC Q9GZV7; P19012: KRT15; NbExp=3; IntAct=EBI-11956675, EBI-739566;
CC Q9GZV7; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-11956675, EBI-3044087;
CC Q9GZV7; Q15323: KRT31; NbExp=3; IntAct=EBI-11956675, EBI-948001;
CC Q9GZV7; Q14525: KRT33B; NbExp=3; IntAct=EBI-11956675, EBI-1049638;
CC Q9GZV7; O76011: KRT34; NbExp=5; IntAct=EBI-11956675, EBI-1047093;
CC Q9GZV7; Q92764: KRT35; NbExp=3; IntAct=EBI-11956675, EBI-1058674;
CC Q9GZV7; O76014: KRT37; NbExp=3; IntAct=EBI-11956675, EBI-1045716;
CC Q9GZV7; Q6A162: KRT40; NbExp=3; IntAct=EBI-11956675, EBI-10171697;
CC Q9GZV7; P78386: KRT85; NbExp=3; IntAct=EBI-11956675, EBI-1049371;
CC Q9GZV7; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-11956675, EBI-10171774;
CC Q9GZV7; Q3LI72: KRTAP19-5; NbExp=3; IntAct=EBI-11956675, EBI-1048945;
CC Q9GZV7; Q9BYR9: KRTAP2-4; NbExp=3; IntAct=EBI-11956675, EBI-14065470;
CC Q9GZV7; Q3LI66: KRTAP6-2; NbExp=3; IntAct=EBI-11956675, EBI-11962084;
CC Q9GZV7; Q9BYQ4: KRTAP9-2; NbExp=3; IntAct=EBI-11956675, EBI-1044640;
CC Q9GZV7; Q99750: MDFI; NbExp=3; IntAct=EBI-11956675, EBI-724076;
CC Q9GZV7; Q9UJV3-2: MID2; NbExp=3; IntAct=EBI-11956675, EBI-10172526;
CC Q9GZV7; Q5JR59-3: MTUS2; NbExp=3; IntAct=EBI-11956675, EBI-11522433;
CC Q9GZV7; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-11956675, EBI-22310682;
CC Q9GZV7; O43482: OIP5; NbExp=3; IntAct=EBI-11956675, EBI-536879;
CC Q9GZV7; Q99471: PFDN5; NbExp=3; IntAct=EBI-11956675, EBI-357275;
CC Q9GZV7; Q8IYS1: PM20D2; NbExp=3; IntAct=EBI-11956675, EBI-11339910;
CC Q9GZV7; Q8ND90: PNMA1; NbExp=3; IntAct=EBI-11956675, EBI-302345;
CC Q9GZV7; Q9UL42: PNMA2; NbExp=3; IntAct=EBI-11956675, EBI-302355;
CC Q9GZV7; Q96T49: PPP1R16B; NbExp=3; IntAct=EBI-11956675, EBI-10293968;
CC Q9GZV7; O43586: PSTPIP1; NbExp=3; IntAct=EBI-11956675, EBI-1050964;
CC Q9GZV7; P55735-3: SEC13; NbExp=3; IntAct=EBI-11956675, EBI-12235008;
CC Q9GZV7; Q12800: TFCP2; NbExp=3; IntAct=EBI-11956675, EBI-717422;
CC Q9GZV7; P36406: TRIM23; NbExp=3; IntAct=EBI-11956675, EBI-740098;
CC Q9GZV7; P14373: TRIM27; NbExp=3; IntAct=EBI-11956675, EBI-719493;
CC Q9GZV7; Q2TAL6: VWC2; NbExp=3; IntAct=EBI-11956675, EBI-11957238;
CC Q9GZV7; Q9H0C1: ZMYND12; NbExp=3; IntAct=EBI-11956675, EBI-12030590;
CC Q9GZV7; Q7Z4V0: ZNF438; NbExp=3; IntAct=EBI-11956675, EBI-11962468;
CC Q9GZV7; Q8N720: ZNF655; NbExp=3; IntAct=EBI-11956675, EBI-625509;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250|UniProtKB:Q9ESM3}.
CC -!- TISSUE SPECIFICITY: Expressed only in adult brain.
CC {ECO:0000269|PubMed:11027579}.
CC -!- SIMILARITY: Belongs to the HAPLN family. {ECO:0000305}.
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DR EMBL; AB049061; BAB17669.1; -; Genomic_DNA.
DR EMBL; AB049054; BAB17662.1; -; mRNA.
DR EMBL; AK090712; BAG52217.1; -; mRNA.
DR EMBL; CH471121; EAW52933.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW52934.1; -; Genomic_DNA.
DR EMBL; BC029864; AAH29864.1; -; mRNA.
DR CCDS; CCDS1148.1; -.
DR PIR; JC7505; JC7505.
DR RefSeq; NP_068589.1; NM_021817.2.
DR RefSeq; XP_005245472.1; XM_005245415.2.
DR RefSeq; XP_011508155.1; XM_011509853.2.
DR RefSeq; XP_016857509.1; XM_017002020.1.
DR AlphaFoldDB; Q9GZV7; -.
DR SMR; Q9GZV7; -.
DR BioGRID; 121916; 47.
DR IntAct; Q9GZV7; 42.
DR STRING; 9606.ENSP00000255039; -.
DR BioMuta; HAPLN2; -.
DR DMDM; 20177844; -.
DR MassIVE; Q9GZV7; -.
DR MaxQB; Q9GZV7; -.
DR PaxDb; Q9GZV7; -.
DR PeptideAtlas; Q9GZV7; -.
DR PRIDE; Q9GZV7; -.
DR ProteomicsDB; 80158; -.
DR Antibodypedia; 34222; 86 antibodies from 24 providers.
DR DNASU; 60484; -.
DR Ensembl; ENST00000255039.6; ENSP00000255039.1; ENSG00000132702.13.
DR GeneID; 60484; -.
DR KEGG; hsa:60484; -.
DR MANE-Select; ENST00000255039.6; ENSP00000255039.1; NM_021817.3; NP_068589.1.
DR UCSC; uc001fpn.1; human.
DR CTD; 60484; -.
DR DisGeNET; 60484; -.
DR GeneCards; HAPLN2; -.
DR HGNC; HGNC:17410; HAPLN2.
DR HPA; ENSG00000132702; Tissue enriched (brain).
DR MIM; 619726; gene.
DR neXtProt; NX_Q9GZV7; -.
DR OpenTargets; ENSG00000132702; -.
DR PharmGKB; PA134878581; -.
DR VEuPathDB; HostDB:ENSG00000132702; -.
DR eggNOG; ENOG502QV1D; Eukaryota.
DR GeneTree; ENSGT00940000161384; -.
DR HOGENOM; CLU_052285_1_0_1; -.
DR InParanoid; Q9GZV7; -.
DR OMA; GQACRNH; -.
DR OrthoDB; 743812at2759; -.
DR PhylomeDB; Q9GZV7; -.
DR TreeFam; TF332134; -.
DR PathwayCommons; Q9GZV7; -.
DR SignaLink; Q9GZV7; -.
DR BioGRID-ORCS; 60484; 144 hits in 1064 CRISPR screens.
DR ChiTaRS; HAPLN2; human.
DR GeneWiki; HAPLN2; -.
DR GenomeRNAi; 60484; -.
DR Pharos; Q9GZV7; Tbio.
DR PRO; PR:Q9GZV7; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9GZV7; protein.
DR Bgee; ENSG00000132702; Expressed in C1 segment of cervical spinal cord and 112 other tissues.
DR ExpressionAtlas; Q9GZV7; baseline and differential.
DR Genevisible; Q9GZV7; HS.
DR GO; GO:0031012; C:extracellular matrix; ISS:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0033268; C:node of Ranvier; IEA:Ensembl.
DR GO; GO:0005540; F:hyaluronic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR GO; GO:0008065; P:establishment of blood-nerve barrier; IEA:Ensembl.
DR GO; GO:0085029; P:extracellular matrix assembly; IEA:Ensembl.
DR GO; GO:0001501; P:skeletal system development; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.10.100.10; -; 2.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR000538; Link_dom.
DR Pfam; PF07686; V-set; 1.
DR Pfam; PF00193; Xlink; 2.
DR PRINTS; PR01265; LINKMODULE.
DR SMART; SM00409; IG; 1.
DR SMART; SM00406; IGv; 1.
DR SMART; SM00445; LINK; 2.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF56436; SSF56436; 2.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS01241; LINK_1; 2.
DR PROSITE; PS50963; LINK_2; 2.
PE 1: Evidence at protein level;
KW Disulfide bond; Extracellular matrix; Hyaluronic acid;
KW Immunoglobulin domain; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..340
FT /note="Hyaluronan and proteoglycan link protein 2"
FT /id="PRO_0000013187"
FT DOMAIN 34..144
FT /note="Ig-like V-type"
FT DOMAIN 148..242
FT /note="Link 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00323"
FT DOMAIN 245..338
FT /note="Link 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00323"
FT DISULFID 57..128
FT /evidence="ECO:0000250"
FT DISULFID 170..240
FT /evidence="ECO:0000250"
FT DISULFID 194..215
FT /evidence="ECO:0000250"
FT DISULFID 265..336
FT /evidence="ECO:0000250"
FT DISULFID 290..311
FT /evidence="ECO:0000250"
SQ SEQUENCE 340 AA; 37775 MW; 62CCC1D86385F5C7 CRC64;
MPGWLTLPTL CRFLLWAFTI FHKAQGDPAS HPGPHYLLPP IHEVIHSHRG ATATLPCVLG
TTPPSYKVRW SKVEPGELRE TLILITNGLH ARGYGPLGGR ARMRRGHRLD ASLVIAGVRL
EDEGRYRCEL INGIEDESVA LTLSLEGVVF PYQPSRGRYQ FNYYEAKQAC EEQDGRLATY
SQLYQAWTEG LDWCNAGWLL EGSVRYPVLT ARAPCGGRGR PGIRSYGPRD RMRDRYDAFC
FTSALAGQVF FVPGRLTLSE AHAACRRRGA VVAKVGHLYA AWKFSGLDQC DGGWLADGSV
RFPITTPRPR CGGLPDPGVR SFGFPRPQQA AYGTYCYAEN
