HPLN3_HUMAN
ID HPLN3_HUMAN Reviewed; 360 AA.
AC Q96S86; A8K7P0;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Hyaluronan and proteoglycan link protein 3;
DE Flags: Precursor;
GN Name=HAPLN3; Synonyms=EXLD1; ORFNames=UNQ238/PRO271;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606 {ECO:0000312|EMBL:AAK67639.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=12663660; DOI=10.1074/jbc.m213100200;
RA Spicer A.P., Joo A., Bowling R.A. Jr.;
RT "A hyaluronan binding link protein gene family whose members are physically
RT linked adjacent to chondroitin sulfate proteoglycan core protein genes: the
RT missing links.";
RL J. Biol. Chem. 278:21083-21091(2003).
RN [2] {ECO:0000312|EMBL:AAK67639.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Li N., Zhang W., Wan T., Zhang M., Cao X.;
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5] {ECO:0000312|EMBL:AAK67639.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas {ECO:0000312|EMBL:AAH62320.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May function in hyaluronic acid binding.
CC {ECO:0000303|PubMed:12663660}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Widely expressed with highest levels in spleen and
CC placenta. {ECO:0000269|PubMed:12663660}.
CC -!- SIMILARITY: Belongs to the HAPLN family. {ECO:0000305}.
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DR EMBL; AY262759; AAP22051.1; -; mRNA.
DR EMBL; AY037161; AAK67639.1; -; mRNA.
DR EMBL; AY358500; AAQ88864.1; -; mRNA.
DR EMBL; AK292055; BAF84744.1; -; mRNA.
DR EMBL; CH471101; EAX02022.1; -; Genomic_DNA.
DR EMBL; BC062320; AAH62320.1; -; mRNA.
DR CCDS; CCDS10346.1; -.
DR RefSeq; NP_001294881.1; NM_001307952.1.
DR RefSeq; NP_839946.1; NM_178232.3.
DR AlphaFoldDB; Q96S86; -.
DR SMR; Q96S86; -.
DR BioGRID; 126950; 56.
DR IntAct; Q96S86; 16.
DR STRING; 9606.ENSP00000352606; -.
DR DrugBank; DB08818; Hyaluronic acid.
DR iPTMnet; Q96S86; -.
DR PhosphoSitePlus; Q96S86; -.
DR BioMuta; HAPLN3; -.
DR DMDM; 47605734; -.
DR EPD; Q96S86; -.
DR jPOST; Q96S86; -.
DR MassIVE; Q96S86; -.
DR PaxDb; Q96S86; -.
DR PeptideAtlas; Q96S86; -.
DR PRIDE; Q96S86; -.
DR ProteomicsDB; 78084; -.
DR Antibodypedia; 28522; 115 antibodies from 21 providers.
DR DNASU; 145864; -.
DR Ensembl; ENST00000359595.8; ENSP00000352606.4; ENSG00000140511.12.
DR GeneID; 145864; -.
DR KEGG; hsa:145864; -.
DR MANE-Select; ENST00000359595.8; ENSP00000352606.4; NM_178232.4; NP_839946.1.
DR UCSC; uc002bnc.4; human.
DR CTD; 145864; -.
DR DisGeNET; 145864; -.
DR GeneCards; HAPLN3; -.
DR HGNC; HGNC:21446; HAPLN3.
DR HPA; ENSG00000140511; Low tissue specificity.
DR neXtProt; NX_Q96S86; -.
DR OpenTargets; ENSG00000140511; -.
DR PharmGKB; PA134919215; -.
DR VEuPathDB; HostDB:ENSG00000140511; -.
DR eggNOG; ENOG502QWFF; Eukaryota.
DR GeneTree; ENSGT00940000159628; -.
DR HOGENOM; CLU_052285_1_0_1; -.
DR InParanoid; Q96S86; -.
DR OrthoDB; 743812at2759; -.
DR PhylomeDB; Q96S86; -.
DR TreeFam; TF332134; -.
DR PathwayCommons; Q96S86; -.
DR SignaLink; Q96S86; -.
DR BioGRID-ORCS; 145864; 11 hits in 1080 CRISPR screens.
DR ChiTaRS; HAPLN3; human.
DR GenomeRNAi; 145864; -.
DR Pharos; Q96S86; Tbio.
DR PRO; PR:Q96S86; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q96S86; protein.
DR Bgee; ENSG00000140511; Expressed in descending thoracic aorta and 127 other tissues.
DR ExpressionAtlas; Q96S86; baseline and differential.
DR Genevisible; Q96S86; HS.
DR GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR GO; GO:0005540; F:hyaluronic acid binding; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0043627; P:response to estrogen; IEA:Ensembl.
DR GO; GO:0001501; P:skeletal system development; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 3.10.100.10; -; 2.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013106; Ig_V-set.
DR InterPro; IPR000538; Link_dom.
DR Pfam; PF07686; V-set; 1.
DR Pfam; PF00193; Xlink; 2.
DR PRINTS; PR01265; LINKMODULE.
DR SMART; SM00409; IG; 1.
DR SMART; SM00406; IGv; 1.
DR SMART; SM00445; LINK; 2.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF56436; SSF56436; 2.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS01241; LINK_1; 2.
DR PROSITE; PS50963; LINK_2; 2.
PE 2: Evidence at transcript level;
KW Disulfide bond; Extracellular matrix; Hyaluronic acid;
KW Immunoglobulin domain; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..360
FT /note="Hyaluronan and proteoglycan link protein 3"
FT /evidence="ECO:0000255"
FT /id="PRO_0000013190"
FT DOMAIN 48..164
FT /note="Ig-like V-type"
FT /evidence="ECO:0000305"
FT DOMAIN 166..261
FT /note="Link 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00323,
FT ECO:0000305"
FT DOMAIN 266..358
FT /note="Link 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00323,
FT ECO:0000305"
FT DISULFID 70..146
FT /evidence="ECO:0000250|UniProtKB:P03994"
FT DISULFID 188..259
FT /evidence="ECO:0000250|UniProtKB:P03994"
FT DISULFID 212..233
FT /evidence="ECO:0000250|UniProtKB:P03994"
FT DISULFID 286..356
FT /evidence="ECO:0000250|UniProtKB:P03994"
FT DISULFID 311..332
FT /evidence="ECO:0000250|UniProtKB:P03994"
SQ SEQUENCE 360 AA; 40894 MW; 3B72AE88D0D9E8DC CRC64;
MGLLLLVPLL LLPGSYGLPF YNGFYYSNSA NDQNLGNGHG KDLLNGVKLV VETPEETLFT
YQGASVILPC RYRYEPALVS PRRVRVKWWK LSENGAPEKD VLVAIGLRHR SFGDYQGRVH
LRQDKEHDVS LEIQDLRLED YGRYRCEVID GLEDESGLVE LELRGVVFPY QSPNGRYQFN
FHEGQQVCAE QAAVVASFEQ LFRAWEEGLD WCNAGWLQDA TVQYPIMLPR QPCGGPGLAP
GVRSYGPRHR RLHRYDVFCF ATALKGRVYY LEHPEKLTLT EAREACQEDD ATIAKVGQLF
AAWKFHGLDR CDAGWLADGS VRYPVVHPHP NCGPPEPGVR SFGFPDPQSR LYGVYCYRQH
