ID   HPNC_RHOPA              Reviewed;         292 AA.
AC   Q6N3F1;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Hydroxysqualene synthase {ECO:0000303|PubMed:26258173};
DE            Short=HSQ synthase {ECO:0000303|PubMed:26258173};
DE            Short=HSQase {ECO:0000303|PubMed:26258173};
DE            EC=4.2.3.156 {ECO:0000269|PubMed:26258173};
GN   Name=hpnC {ECO:0000303|PubMed:26258173};
GN   OrderedLocusNames=RPA3743 {ECO:0000312|EMBL:CAE29184.1};
OS   Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=258594;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-98 / CGA009;
RX   PubMed=14704707; DOI=10.1038/nbt923;
RA   Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA   Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA   Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA   Harrison F.H., Gibson J., Harwood C.S.;
RT   "Complete genome sequence of the metabolically versatile photosynthetic
RT   bacterium Rhodopseudomonas palustris.";
RL   Nat. Biotechnol. 22:55-61(2004).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC   STRAIN=ATCC BAA-98 / CGA009;
RX   PubMed=26258173; DOI=10.1021/acscentsci.5b00115;
RA   Pan J.J., Solbiati J.O., Ramamoorthy G., Hillerich B.S., Seidel R.D.,
RA   Cronan J.E., Almo S.C., Poulter C.D.;
RT   "Biosynthesis of squalene from farnesyl diphosphate in bacteria: three
RT   steps catalyzed by three enzymes.";
RL   ACS Cent. Sci. 1:77-82(2015).
CC   -!- FUNCTION: Involved in the biosynthesis of the hopanoid precursor
CC       squalene (SQ) from farnesyl diphosphate (FPP). Catalyzes the second
CC       step, the conversion of presqualene diphosphate (PSPP) to
CC       hydroxysqualene (HSQ). {ECO:0000269|PubMed:26258173}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + presqualene diphosphate = diphosphate + hydroxysqualene;
CC         Xref=Rhea:RHEA:47984, ChEBI:CHEBI:15377, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57310, ChEBI:CHEBI:88123; EC=4.2.3.156;
CC         Evidence={ECO:0000269|PubMed:26258173};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; hopanoid biosynthesis.
CC       {ECO:0000269|PubMed:26258173}.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family. HpnC
CC       subfamily. {ECO:0000305}.
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DR   EMBL; BX572605; CAE29184.1; -; Genomic_DNA.
DR   RefSeq; WP_011159281.1; NC_005296.1.
DR   AlphaFoldDB; Q6N3F1; -.
DR   SMR; Q6N3F1; -.
DR   STRING; 258594.RPA3743; -.
DR   PRIDE; Q6N3F1; -.
DR   DNASU; 2691711; -.
DR   EnsemblBacteria; CAE29184; CAE29184; RPA3743.
DR   GeneID; 66894849; -.
DR   KEGG; rpa:RPA3743; -.
DR   eggNOG; COG1562; Bacteria.
DR   HOGENOM; CLU_037269_0_1_5; -.
DR   OMA; DYCRYSA; -.
DR   PhylomeDB; Q6N3F1; -.
DR   BioCyc; MetaCyc:MON-19571; -.
DR   BioCyc; RPAL258594:TX73_RS19105-MON; -.
DR   BRENDA; 4.2.3.156; 5412.
DR   UniPathway; UPA00337; -.
DR   Proteomes; UP000001426; Chromosome.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR017827; HSQ_synthase_HpnC.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   PANTHER; PTHR31480:SF17; PTHR31480:SF17; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   TIGRFAMs; TIGR03464; HpnC; 1.
PE   1: Evidence at protein level;
KW   Lyase; Reference proteome.
FT   CHAIN           1..292
FT                   /note="Hydroxysqualene synthase"
FT                   /id="PRO_0000441691"
SQ   SEQUENCE   292 AA;  32831 MW;  2FE334F6C3404F82 CRC64;
     MTSASELRSG KTHRDENFPV ASWIIHPRHR DLILAFYNFV RTADDIADHE MLDGDTKLEY
     LDLLEAELLG RGETQPEAVH LRRALAERGM PPRHALDLLT AFRMDVTKLR YEDWDEVIHY
     CRYSAMPVGR FMLDVHGEST TTWQASDALC AGLQINNHLQ DCGKDYRTLN RVYLPRDVLD
     AAGAKVEDLG LQKSSPALLK CLQGLAVRTA SLLGDGRPLA AEIKDYRLGL EVSVIQAYAD
     RIVRMLQTRD PLSERVHLKP IEFVIASFGA MSSEIVRRSF GKGPVSHPAP RA